Neurogenesis and gliogenesis modulation in cerebral ischemia by CDK5 RNAi-based therapy
Introduction: Cerebral ischemia is the third cause of death risk in Colombia and the first cause of physical disability worldwide. Different studies on the silencing of the cyclin-dependent kinase 5 (CDK5) have shown that reducing its activity is beneficial in ischemic contexts. However, its effect...
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ftdoajarticles:oai:doaj.org/article:ac697ea40c044d7e9f37868274b2d3cf 2023-05-15T15:11:40+02:00 Neurogenesis and gliogenesis modulation in cerebral ischemia by CDK5 RNAi-based therapy Juan Ignacio Muñoz-Manco Johanna Andrea Gutiérrez-Vargas Gloria Patricia Cardona-Gómez 2018-09-01T00:00:00Z https://doi.org/10.7705/biomedica.v38i4.3800 https://doaj.org/article/ac697ea40c044d7e9f37868274b2d3cf EN ES eng spa Instituto Nacional de Salud https://www.revistabiomedica.org/index.php/biomedica/article/view/3800 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.v38i4.3800 https://doaj.org/article/ac697ea40c044d7e9f37868274b2d3cf Biomédica: revista del Instituto Nacional de Salud, Vol 38, Iss 3, Pp 388-397 (2018) Brain ischemia neurogenesis cyclin-dependent kinase 5 Medicine R Arctic medicine. Tropical medicine RC955-962 article 2018 ftdoajarticles https://doi.org/10.7705/biomedica.v38i4.3800 2022-12-30T21:16:42Z Introduction: Cerebral ischemia is the third cause of death risk in Colombia and the first cause of physical disability worldwide. Different studies on the silencing of the cyclin-dependent kinase 5 (CDK5) have shown that reducing its activity is beneficial in ischemic contexts. However, its effect on neural cell production after cerebral ischemia has not been well studied yet. Objective: To evaluate CDK5 silencing on the production of neurons and astrocytes after a focal cerebral ischemia in rats. Materials and methods: We used 40 eight-week-old male Wistar rats. Both sham and ischemia groups were transduced at CA1 hippocampal region with an adeno-associated viral vector using a noninterfering (shSCRmiR) and an interfering sequence for CDK5 (shCDK5miR). We injected 50 mg/kg of bromodeoxyuridine intraperitoneally from hour 24 to day 7 post-ischemia. We assessed the neurological abilities during the next 15 days and we measured the immunoreactivity of bromodeoxyuridine (BrdU), doublecortin (DCX), NeuN, and glial fibrillary acid protein (GFAP) from day 15 to day 30 post-ischemia. Results: Our findings showed that CDK5miR-treated ischemic animals improved their neurological score and presented increased BrdU+ cells 15 days after ischemia, which correlated with higher DCX and lower GFAP fluorescence intensities, and, although mature neurons populations did not change, GFAP immunoreactivity was still significantly reduced at 30 days post-ischemia in comparison with untreated ischemic groups. Conclusion: CDK5miR therapy generated the neurological recovery of ischemic rats associated with the induction of immature neurons proliferation and the reduction of GFAP reactivity at short and longterm post-ischemia. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Biomédica 38 3 388 397 |
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Brain ischemia neurogenesis cyclin-dependent kinase 5 Medicine R Arctic medicine. Tropical medicine RC955-962 |
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Brain ischemia neurogenesis cyclin-dependent kinase 5 Medicine R Arctic medicine. Tropical medicine RC955-962 Juan Ignacio Muñoz-Manco Johanna Andrea Gutiérrez-Vargas Gloria Patricia Cardona-Gómez Neurogenesis and gliogenesis modulation in cerebral ischemia by CDK5 RNAi-based therapy |
topic_facet |
Brain ischemia neurogenesis cyclin-dependent kinase 5 Medicine R Arctic medicine. Tropical medicine RC955-962 |
description |
Introduction: Cerebral ischemia is the third cause of death risk in Colombia and the first cause of physical disability worldwide. Different studies on the silencing of the cyclin-dependent kinase 5 (CDK5) have shown that reducing its activity is beneficial in ischemic contexts. However, its effect on neural cell production after cerebral ischemia has not been well studied yet. Objective: To evaluate CDK5 silencing on the production of neurons and astrocytes after a focal cerebral ischemia in rats. Materials and methods: We used 40 eight-week-old male Wistar rats. Both sham and ischemia groups were transduced at CA1 hippocampal region with an adeno-associated viral vector using a noninterfering (shSCRmiR) and an interfering sequence for CDK5 (shCDK5miR). We injected 50 mg/kg of bromodeoxyuridine intraperitoneally from hour 24 to day 7 post-ischemia. We assessed the neurological abilities during the next 15 days and we measured the immunoreactivity of bromodeoxyuridine (BrdU), doublecortin (DCX), NeuN, and glial fibrillary acid protein (GFAP) from day 15 to day 30 post-ischemia. Results: Our findings showed that CDK5miR-treated ischemic animals improved their neurological score and presented increased BrdU+ cells 15 days after ischemia, which correlated with higher DCX and lower GFAP fluorescence intensities, and, although mature neurons populations did not change, GFAP immunoreactivity was still significantly reduced at 30 days post-ischemia in comparison with untreated ischemic groups. Conclusion: CDK5miR therapy generated the neurological recovery of ischemic rats associated with the induction of immature neurons proliferation and the reduction of GFAP reactivity at short and longterm post-ischemia. |
format |
Article in Journal/Newspaper |
author |
Juan Ignacio Muñoz-Manco Johanna Andrea Gutiérrez-Vargas Gloria Patricia Cardona-Gómez |
author_facet |
Juan Ignacio Muñoz-Manco Johanna Andrea Gutiérrez-Vargas Gloria Patricia Cardona-Gómez |
author_sort |
Juan Ignacio Muñoz-Manco |
title |
Neurogenesis and gliogenesis modulation in cerebral ischemia by CDK5 RNAi-based therapy |
title_short |
Neurogenesis and gliogenesis modulation in cerebral ischemia by CDK5 RNAi-based therapy |
title_full |
Neurogenesis and gliogenesis modulation in cerebral ischemia by CDK5 RNAi-based therapy |
title_fullStr |
Neurogenesis and gliogenesis modulation in cerebral ischemia by CDK5 RNAi-based therapy |
title_full_unstemmed |
Neurogenesis and gliogenesis modulation in cerebral ischemia by CDK5 RNAi-based therapy |
title_sort |
neurogenesis and gliogenesis modulation in cerebral ischemia by cdk5 rnai-based therapy |
publisher |
Instituto Nacional de Salud |
publishDate |
2018 |
url |
https://doi.org/10.7705/biomedica.v38i4.3800 https://doaj.org/article/ac697ea40c044d7e9f37868274b2d3cf |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Biomédica: revista del Instituto Nacional de Salud, Vol 38, Iss 3, Pp 388-397 (2018) |
op_relation |
https://www.revistabiomedica.org/index.php/biomedica/article/view/3800 https://doaj.org/toc/0120-4157 0120-4157 doi:10.7705/biomedica.v38i4.3800 https://doaj.org/article/ac697ea40c044d7e9f37868274b2d3cf |
op_doi |
https://doi.org/10.7705/biomedica.v38i4.3800 |
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Biomédica |
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38 |
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397 |
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