Characterizing the Alteration in Rumen Microbiome and Carbohydrate-Active Enzymes Profile with Forage of Muskoxen Rumen through Comparative Metatranscriptomics
Muskox ( Ovibos moschatus ), as the biggest herbivore in the High Arctic, has been enduring the austere arctic nutritional conditions and has evolved to ingest and digest scarce and high lignified forages to support the growth and reproduce, implying probably harbor a distinct microbial reservoir fo...
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MDPI AG
2021
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Online Access: | https://doi.org/10.3390/microorganisms10010071 https://doaj.org/article/ac67242c49ac4eac87b86921d97afe58 |
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ftdoajarticles:oai:doaj.org/article:ac67242c49ac4eac87b86921d97afe58 2023-05-15T15:01:51+02:00 Characterizing the Alteration in Rumen Microbiome and Carbohydrate-Active Enzymes Profile with Forage of Muskoxen Rumen through Comparative Metatranscriptomics Xiaofeng Wu Chijioke O. Elekwachi Shiping Bai Yuheng Luo Keying Zhang Robert J. Forster 2021-12-01T00:00:00Z https://doi.org/10.3390/microorganisms10010071 https://doaj.org/article/ac67242c49ac4eac87b86921d97afe58 EN eng MDPI AG https://www.mdpi.com/2076-2607/10/1/71 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms10010071 2076-2607 https://doaj.org/article/ac67242c49ac4eac87b86921d97afe58 Microorganisms, Vol 10, Iss 71, p 71 (2021) muskoxen rumen metatranscriptomics microbiome triticale straw brome hay Biology (General) QH301-705.5 article 2021 ftdoajarticles https://doi.org/10.3390/microorganisms10010071 2022-12-30T20:33:26Z Muskox ( Ovibos moschatus ), as the biggest herbivore in the High Arctic, has been enduring the austere arctic nutritional conditions and has evolved to ingest and digest scarce and high lignified forages to support the growth and reproduce, implying probably harbor a distinct microbial reservoir for the deconstruction of plant biomass. Therefore, metagenomics approach was applied to characterize the rumen microbial community and understand the alteration in rumen microbiome of muskoxen fed either triticale straw or brome hay. The difference in the structure of microbial communities including bacteria, archaea, fungi, and protozoa between the two forages was observed at the taxonomic level of genus. Further, although the highly abundant phylotypes in muskoxen rumen fed either triticale straw or brome hay were almost the same, the selective enrichment different phylotypes for fiber degrading, soluble substrates fermenting, electron and hydrogen scavenging through methanogenesis, acetogenesis, propionogenesis, and sulfur-reducing was also noticed. Specifically, triticale straw with higher content of fiber, cellulose selectively enriched more lignocellulolytic taxa and electron transferring taxa, while brome hay with higher nitrogen content selectively enriched more families and genera for degradable substrates-digesting. Intriguingly, the carbohydrate-active enzyme profile suggested an over representation and diversity of putative glycoside hydrolases (GHs) in the animals fed on triticale straw. The majority of the cellulases belonged to fiver GH families (i.e., GH5, GH6, GH9, GH45, and GH48) and were primarily synthesized by Ruminococcus , Piromyces , Neocallimastix , and Fibrobacter . Abundance of major genes coding for hemicellulose digestion was higher than cellulose mainly including GH8, GH10, GH16, GH26, and GH30, and these enzymes were produced by members of the genera Fibrobacter , Ruminococcus , and Clostridium . Oligosaccharides were mainly of the GH1, GH2, GH3, and GH31 types and were associated with ... Article in Journal/Newspaper Arctic muskox ovibos moschatus Directory of Open Access Journals: DOAJ Articles Arctic Microorganisms 10 1 71 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
muskoxen rumen metatranscriptomics microbiome triticale straw brome hay Biology (General) QH301-705.5 |
spellingShingle |
muskoxen rumen metatranscriptomics microbiome triticale straw brome hay Biology (General) QH301-705.5 Xiaofeng Wu Chijioke O. Elekwachi Shiping Bai Yuheng Luo Keying Zhang Robert J. Forster Characterizing the Alteration in Rumen Microbiome and Carbohydrate-Active Enzymes Profile with Forage of Muskoxen Rumen through Comparative Metatranscriptomics |
topic_facet |
muskoxen rumen metatranscriptomics microbiome triticale straw brome hay Biology (General) QH301-705.5 |
description |
Muskox ( Ovibos moschatus ), as the biggest herbivore in the High Arctic, has been enduring the austere arctic nutritional conditions and has evolved to ingest and digest scarce and high lignified forages to support the growth and reproduce, implying probably harbor a distinct microbial reservoir for the deconstruction of plant biomass. Therefore, metagenomics approach was applied to characterize the rumen microbial community and understand the alteration in rumen microbiome of muskoxen fed either triticale straw or brome hay. The difference in the structure of microbial communities including bacteria, archaea, fungi, and protozoa between the two forages was observed at the taxonomic level of genus. Further, although the highly abundant phylotypes in muskoxen rumen fed either triticale straw or brome hay were almost the same, the selective enrichment different phylotypes for fiber degrading, soluble substrates fermenting, electron and hydrogen scavenging through methanogenesis, acetogenesis, propionogenesis, and sulfur-reducing was also noticed. Specifically, triticale straw with higher content of fiber, cellulose selectively enriched more lignocellulolytic taxa and electron transferring taxa, while brome hay with higher nitrogen content selectively enriched more families and genera for degradable substrates-digesting. Intriguingly, the carbohydrate-active enzyme profile suggested an over representation and diversity of putative glycoside hydrolases (GHs) in the animals fed on triticale straw. The majority of the cellulases belonged to fiver GH families (i.e., GH5, GH6, GH9, GH45, and GH48) and were primarily synthesized by Ruminococcus , Piromyces , Neocallimastix , and Fibrobacter . Abundance of major genes coding for hemicellulose digestion was higher than cellulose mainly including GH8, GH10, GH16, GH26, and GH30, and these enzymes were produced by members of the genera Fibrobacter , Ruminococcus , and Clostridium . Oligosaccharides were mainly of the GH1, GH2, GH3, and GH31 types and were associated with ... |
format |
Article in Journal/Newspaper |
author |
Xiaofeng Wu Chijioke O. Elekwachi Shiping Bai Yuheng Luo Keying Zhang Robert J. Forster |
author_facet |
Xiaofeng Wu Chijioke O. Elekwachi Shiping Bai Yuheng Luo Keying Zhang Robert J. Forster |
author_sort |
Xiaofeng Wu |
title |
Characterizing the Alteration in Rumen Microbiome and Carbohydrate-Active Enzymes Profile with Forage of Muskoxen Rumen through Comparative Metatranscriptomics |
title_short |
Characterizing the Alteration in Rumen Microbiome and Carbohydrate-Active Enzymes Profile with Forage of Muskoxen Rumen through Comparative Metatranscriptomics |
title_full |
Characterizing the Alteration in Rumen Microbiome and Carbohydrate-Active Enzymes Profile with Forage of Muskoxen Rumen through Comparative Metatranscriptomics |
title_fullStr |
Characterizing the Alteration in Rumen Microbiome and Carbohydrate-Active Enzymes Profile with Forage of Muskoxen Rumen through Comparative Metatranscriptomics |
title_full_unstemmed |
Characterizing the Alteration in Rumen Microbiome and Carbohydrate-Active Enzymes Profile with Forage of Muskoxen Rumen through Comparative Metatranscriptomics |
title_sort |
characterizing the alteration in rumen microbiome and carbohydrate-active enzymes profile with forage of muskoxen rumen through comparative metatranscriptomics |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/microorganisms10010071 https://doaj.org/article/ac67242c49ac4eac87b86921d97afe58 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic muskox ovibos moschatus |
genre_facet |
Arctic muskox ovibos moschatus |
op_source |
Microorganisms, Vol 10, Iss 71, p 71 (2021) |
op_relation |
https://www.mdpi.com/2076-2607/10/1/71 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms10010071 2076-2607 https://doaj.org/article/ac67242c49ac4eac87b86921d97afe58 |
op_doi |
https://doi.org/10.3390/microorganisms10010071 |
container_title |
Microorganisms |
container_volume |
10 |
container_issue |
1 |
container_start_page |
71 |
_version_ |
1766333856882884608 |