Impact of antibacterial drugs on human serum paraoxonase-1 (hPON1) activity: an in vitro study

• Published in: Asian Pacific Journal of Tropical Biomedicine
• Main Authors: Hakan Söyüt, Elif Duygu Kaya, Şükrü Beydemir
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wolters Kluwer Medknow Publications 2014
• Subjects: Paraoxonase; Inhibition; Meropenem trihydrate; Piperacillin sodium; Cefoperazone sodium; Arctic medicine. Tropical medicine; RC955-962; Biology (General); QH301-705.5; Arctic
• Online Access: https://doi.org/10.12980/APJTB.4.2014APJTB-2014-0059; https://doaj.org/article/abfe08c0e348430e8f04beb65f5969bf

Objective: To investigate the in vitro effects of the antibacterial drugs, meropenem trihydrate, piperacillin sodium, and cefoperazone sodium, on the activity of human serum paraoxonase (hPON1). Methods: hPON1 was purified from human serum using simple chromatographic methods, including DEAE-Sephadex anion exchange and Sephadex G-200 gel filtration chromatography. Results: The three antibacterial drugs decreased in vitro hPON1 activity. Inhibition mechanisms meropenem trihydrate was noncompetitive while piperacillin sodium and cefoperazone sodium were competitive. Conclusions: Our results showed that antibacterial drugs significantly inhibit hPON1 activity, both in vitro, with rank order meropenem trihydrate piperacillin sodium cefoperazone sodium in vitro.