Bothrops jararacussu venom-induced neuromuscular blockade inhibited by Casearia gossypiosperma Briquet hydroalcoholic extract
The hydroalcoholic extract of Casearia gossypiosperma Briquet (Flacourtiaceae) was standardized for the first time through quality control procedures including pharmacognostic methods, fingerprint chromatograms, defined amounts of marker substances and physicochemical characteristics. The pharmacolo...
Published in: | Journal of Venomous Animals and Toxins including Tropical Diseases |
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Online Access: | https://doi.org/10.1590/S1678-91992010000300009 https://doaj.org/article/abf2a12dd1d44e2e8675da888f01da12 |
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ftdoajarticles:oai:doaj.org/article:abf2a12dd1d44e2e8675da888f01da12 2023-05-15T15:04:14+02:00 Bothrops jararacussu venom-induced neuromuscular blockade inhibited by Casearia gossypiosperma Briquet hydroalcoholic extract TM Camargo VS Nazato MG Silva JC Cogo FC Groppo Y Oshima-Franco 2010-01-01T00:00:00Z https://doi.org/10.1590/S1678-91992010000300009 https://doaj.org/article/abf2a12dd1d44e2e8675da888f01da12 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300009 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992010000300009 1678-9199 https://doaj.org/article/abf2a12dd1d44e2e8675da888f01da12 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 16, Iss 3, Pp 432-441 (2010) antibothropic extract Bothrops jararacussu Casearia gossypiosperma Briquet snake venoms Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2010 ftdoajarticles https://doi.org/10.1590/S1678-91992010000300009 2022-12-30T21:14:53Z The hydroalcoholic extract of Casearia gossypiosperma Briquet (Flacourtiaceae) was standardized for the first time through quality control procedures including pharmacognostic methods, fingerprint chromatograms, defined amounts of marker substances and physicochemical characteristics. The pharmacological activity of C. gossypiosperma (Cg) hydroalcoholic extract was assayed by a traditional in vitro test, which involved irreversible neuromuscular blockade induced by Bothrops jararacussu (Bjssu) venom (60 µg/mL) in mouse phrenic nerve-diaphragm preparations. Bjssu venom blocked muscle activity for 26 (± 2.0) minutes (n = 6). Cg extract (0.1 mg/mL) induced changes on the baseline muscle activity without impairing the muscle function and inhibited 87.6% (± 1.8) (n = 6) of the Bjssu venom-induced blockade. Both flavonoids (0.624 g%) and polyphenols (4.63 g%) from the extract were spectrophotometrically quantified. Therefore, the present study confirms the antibothropic activity of Cg extract, supporting the ethnomedical use of Casearia sp. in the treatment of snakebite victims. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 16 3 432 441 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
antibothropic extract Bothrops jararacussu Casearia gossypiosperma Briquet snake venoms Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
antibothropic extract Bothrops jararacussu Casearia gossypiosperma Briquet snake venoms Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 TM Camargo VS Nazato MG Silva JC Cogo FC Groppo Y Oshima-Franco Bothrops jararacussu venom-induced neuromuscular blockade inhibited by Casearia gossypiosperma Briquet hydroalcoholic extract |
topic_facet |
antibothropic extract Bothrops jararacussu Casearia gossypiosperma Briquet snake venoms Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
The hydroalcoholic extract of Casearia gossypiosperma Briquet (Flacourtiaceae) was standardized for the first time through quality control procedures including pharmacognostic methods, fingerprint chromatograms, defined amounts of marker substances and physicochemical characteristics. The pharmacological activity of C. gossypiosperma (Cg) hydroalcoholic extract was assayed by a traditional in vitro test, which involved irreversible neuromuscular blockade induced by Bothrops jararacussu (Bjssu) venom (60 µg/mL) in mouse phrenic nerve-diaphragm preparations. Bjssu venom blocked muscle activity for 26 (± 2.0) minutes (n = 6). Cg extract (0.1 mg/mL) induced changes on the baseline muscle activity without impairing the muscle function and inhibited 87.6% (± 1.8) (n = 6) of the Bjssu venom-induced blockade. Both flavonoids (0.624 g%) and polyphenols (4.63 g%) from the extract were spectrophotometrically quantified. Therefore, the present study confirms the antibothropic activity of Cg extract, supporting the ethnomedical use of Casearia sp. in the treatment of snakebite victims. |
format |
Article in Journal/Newspaper |
author |
TM Camargo VS Nazato MG Silva JC Cogo FC Groppo Y Oshima-Franco |
author_facet |
TM Camargo VS Nazato MG Silva JC Cogo FC Groppo Y Oshima-Franco |
author_sort |
TM Camargo |
title |
Bothrops jararacussu venom-induced neuromuscular blockade inhibited by Casearia gossypiosperma Briquet hydroalcoholic extract |
title_short |
Bothrops jararacussu venom-induced neuromuscular blockade inhibited by Casearia gossypiosperma Briquet hydroalcoholic extract |
title_full |
Bothrops jararacussu venom-induced neuromuscular blockade inhibited by Casearia gossypiosperma Briquet hydroalcoholic extract |
title_fullStr |
Bothrops jararacussu venom-induced neuromuscular blockade inhibited by Casearia gossypiosperma Briquet hydroalcoholic extract |
title_full_unstemmed |
Bothrops jararacussu venom-induced neuromuscular blockade inhibited by Casearia gossypiosperma Briquet hydroalcoholic extract |
title_sort |
bothrops jararacussu venom-induced neuromuscular blockade inhibited by casearia gossypiosperma briquet hydroalcoholic extract |
publisher |
SciELO |
publishDate |
2010 |
url |
https://doi.org/10.1590/S1678-91992010000300009 https://doaj.org/article/abf2a12dd1d44e2e8675da888f01da12 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 16, Iss 3, Pp 432-441 (2010) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300009 https://doaj.org/toc/1678-9199 doi:10.1590/S1678-91992010000300009 1678-9199 https://doaj.org/article/abf2a12dd1d44e2e8675da888f01da12 |
op_doi |
https://doi.org/10.1590/S1678-91992010000300009 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
16 |
container_issue |
3 |
container_start_page |
432 |
op_container_end_page |
441 |
_version_ |
1766336034038087680 |