Interferon-gamma responses to Plasmodium falciparum liver-stage antigen-1 and merozoite-surface protein-1 increase with age in children in a malaria holoendemic area of western Kenya
Abstract Background In areas of high-level, year-round malaria transmission, morbidity and mortality due to malaria decrease after the first two to three years of life. This reduction may be related to the development of cellular immunity to specific antigens expressed in the different life-cycle st...
Published in: | Malaria Journal |
---|---|
Main Authors: | , , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
BMC
2003
|
Subjects: | |
Online Access: | https://doi.org/10.1186/1475-2875-2-37 https://doaj.org/article/abf28e64073e40ad9d567b792fc42b2d |
id |
ftdoajarticles:oai:doaj.org/article:abf28e64073e40ad9d567b792fc42b2d |
---|---|
record_format |
openpolar |
spelling |
ftdoajarticles:oai:doaj.org/article:abf28e64073e40ad9d567b792fc42b2d 2023-05-15T15:15:10+02:00 Interferon-gamma responses to Plasmodium falciparum liver-stage antigen-1 and merozoite-surface protein-1 increase with age in children in a malaria holoendemic area of western Kenya Kazura James W Sumba Peter O Chelimo Kiprotich Ofula Ayub V John Chandy C 2003-11-01T00:00:00Z https://doi.org/10.1186/1475-2875-2-37 https://doaj.org/article/abf28e64073e40ad9d567b792fc42b2d EN eng BMC http://www.malariajournal.com/content/2/1/37 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-2-37 1475-2875 https://doaj.org/article/abf28e64073e40ad9d567b792fc42b2d Malaria Journal, Vol 2, Iss 1, p 37 (2003) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2003 ftdoajarticles https://doi.org/10.1186/1475-2875-2-37 2023-01-08T01:36:01Z Abstract Background In areas of high-level, year-round malaria transmission, morbidity and mortality due to malaria decrease after the first two to three years of life. This reduction may be related to the development of cellular immunity to specific antigens expressed in the different life-cycle stages of Plasmodium falciparum . Methods A cross sectional study was conducted to evaluate T cell cytokine responses to the P. falciparum pre-erythrocytic antigen liver-stage antigen-1 (LSA-1) and the blood-stage antigen merozoite-surface protein-1 (MSP-1) in children under five years of age residing in a malaria holoendemic region of western Kenya. Interferon-γ (IFN-γ) and interleukin-10 (IL-10) responses to the LSA-1 T3 peptide (aa 1813–1835) and the MSP-1 aa20–39 peptide were tested in 48 children. Results The proportion of children producing IFN-γ to LSA-1 and to MSP-1 increased with age: in the 0–12, 13–24, 25–36 and 37–48 month age groups, zero, 11.1, 36.4 and 40% of children had IFN-γ responses to LSA-1 (p = 0.019), and 10, 10, 27.7 and 40% of children had IFN-γ responses to MSP-1 (p = 0.07), respectively. In contrast, the proportion of children producing IL-10 to LSA-1 and MSP-1 was similar in all age groups. Conclusion The data suggest that development of IFN-γ responses to LSA-1 and MSP-1 requires increased age and/or repeated exposure, whereas IL-10 responses to these antigens may occur at any age and with limited exposure. The data also demonstrate that by the age of 4 years, children in a malaria holoendemic area develop frequencies of IFN-γ responses to LSA-1 and MSP-1 similar to those seen in adults in the area. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 2 1 37 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Kazura James W Sumba Peter O Chelimo Kiprotich Ofula Ayub V John Chandy C Interferon-gamma responses to Plasmodium falciparum liver-stage antigen-1 and merozoite-surface protein-1 increase with age in children in a malaria holoendemic area of western Kenya |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background In areas of high-level, year-round malaria transmission, morbidity and mortality due to malaria decrease after the first two to three years of life. This reduction may be related to the development of cellular immunity to specific antigens expressed in the different life-cycle stages of Plasmodium falciparum . Methods A cross sectional study was conducted to evaluate T cell cytokine responses to the P. falciparum pre-erythrocytic antigen liver-stage antigen-1 (LSA-1) and the blood-stage antigen merozoite-surface protein-1 (MSP-1) in children under five years of age residing in a malaria holoendemic region of western Kenya. Interferon-γ (IFN-γ) and interleukin-10 (IL-10) responses to the LSA-1 T3 peptide (aa 1813–1835) and the MSP-1 aa20–39 peptide were tested in 48 children. Results The proportion of children producing IFN-γ to LSA-1 and to MSP-1 increased with age: in the 0–12, 13–24, 25–36 and 37–48 month age groups, zero, 11.1, 36.4 and 40% of children had IFN-γ responses to LSA-1 (p = 0.019), and 10, 10, 27.7 and 40% of children had IFN-γ responses to MSP-1 (p = 0.07), respectively. In contrast, the proportion of children producing IL-10 to LSA-1 and MSP-1 was similar in all age groups. Conclusion The data suggest that development of IFN-γ responses to LSA-1 and MSP-1 requires increased age and/or repeated exposure, whereas IL-10 responses to these antigens may occur at any age and with limited exposure. The data also demonstrate that by the age of 4 years, children in a malaria holoendemic area develop frequencies of IFN-γ responses to LSA-1 and MSP-1 similar to those seen in adults in the area. |
format |
Article in Journal/Newspaper |
author |
Kazura James W Sumba Peter O Chelimo Kiprotich Ofula Ayub V John Chandy C |
author_facet |
Kazura James W Sumba Peter O Chelimo Kiprotich Ofula Ayub V John Chandy C |
author_sort |
Kazura James W |
title |
Interferon-gamma responses to Plasmodium falciparum liver-stage antigen-1 and merozoite-surface protein-1 increase with age in children in a malaria holoendemic area of western Kenya |
title_short |
Interferon-gamma responses to Plasmodium falciparum liver-stage antigen-1 and merozoite-surface protein-1 increase with age in children in a malaria holoendemic area of western Kenya |
title_full |
Interferon-gamma responses to Plasmodium falciparum liver-stage antigen-1 and merozoite-surface protein-1 increase with age in children in a malaria holoendemic area of western Kenya |
title_fullStr |
Interferon-gamma responses to Plasmodium falciparum liver-stage antigen-1 and merozoite-surface protein-1 increase with age in children in a malaria holoendemic area of western Kenya |
title_full_unstemmed |
Interferon-gamma responses to Plasmodium falciparum liver-stage antigen-1 and merozoite-surface protein-1 increase with age in children in a malaria holoendemic area of western Kenya |
title_sort |
interferon-gamma responses to plasmodium falciparum liver-stage antigen-1 and merozoite-surface protein-1 increase with age in children in a malaria holoendemic area of western kenya |
publisher |
BMC |
publishDate |
2003 |
url |
https://doi.org/10.1186/1475-2875-2-37 https://doaj.org/article/abf28e64073e40ad9d567b792fc42b2d |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 2, Iss 1, p 37 (2003) |
op_relation |
http://www.malariajournal.com/content/2/1/37 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-2-37 1475-2875 https://doaj.org/article/abf28e64073e40ad9d567b792fc42b2d |
op_doi |
https://doi.org/10.1186/1475-2875-2-37 |
container_title |
Malaria Journal |
container_volume |
2 |
container_issue |
1 |
container_start_page |
37 |
_version_ |
1766345551964536832 |