The glutaminase (CgGLS-1) mediates anti-bacterial immunity by prompting cytokine synthesis and hemocyte apoptosis in Pacific oyster Crassostrea gigas
Abstract Glutaminase, an amidohydrolase enzyme that hydrolyzes glutamine to glutamate, plays crucial roles in various immunomodulatory processes such as cell apoptosis, proliferation, migration, and secretion of cytokines. In the present study, a glutaminase homologue (designated as CgGLS-1) was ide...
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ftdoajarticles:oai:doaj.org/article:abf18a1f11f444348f7925d4c451342f 2023-05-15T15:58:18+02:00 The glutaminase (CgGLS-1) mediates anti-bacterial immunity by prompting cytokine synthesis and hemocyte apoptosis in Pacific oyster Crassostrea gigas Yage Liang Meijia Li Zhaoqun Liu Yuanmei Li Lingling Wang Linsheng Song 2021-01-01T00:00:00Z https://doi.org/10.1038/s41598-020-80552-2 https://doaj.org/article/abf18a1f11f444348f7925d4c451342f EN eng Nature Portfolio https://doi.org/10.1038/s41598-020-80552-2 https://doaj.org/toc/2045-2322 doi:10.1038/s41598-020-80552-2 2045-2322 https://doaj.org/article/abf18a1f11f444348f7925d4c451342f Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021) Medicine R Science Q article 2021 ftdoajarticles https://doi.org/10.1038/s41598-020-80552-2 2022-12-31T05:25:36Z Abstract Glutaminase, an amidohydrolase enzyme that hydrolyzes glutamine to glutamate, plays crucial roles in various immunomodulatory processes such as cell apoptosis, proliferation, migration, and secretion of cytokines. In the present study, a glutaminase homologue (designated as CgGLS-1) was identified from Pacific oyster Crassostrea gigas, whose open reading frame was of 1836 bp. CgGLS-1 exhibited high sequence identity with vertebrate kidney-type GLS, and closely clustered with their homologues from mollusc C. virginica. The enzyme activity of recombinant CgGLS-1 protein (rCgGLS-1) was estimated to be 1.705 U/mg. CgGLS-1 mRNA was constitutively expressed in all the tested tissues of oysters, with the highest expression level in hemocytes. CgGLS-1 mRNA expression in hemocytes was significantly up-regulated and peaked at 6 h (2.07-fold, p < 0.01) after lipopolysaccharide (LPS) stimulation. The CgGLS-1 protein was mainly distributed in the cytoplasm with a significant co-location with mitochondria in oyster hemocytes. The content of Glu in the oyster serum was significantly decreased after the inhibition of CgGLS-1 using specific inhibitor Bis-2- [5-(phenyl acetamido)-1,3,4-thiadiazol-2-yl] ethyl sulfide (BPTES), and the expression levels of CgmGluR6, CgAP-1, cytokines CgIL17-5 and CgTNF-1 were significantly decreased after BPTES and LPS stimulation. The transcripts of CgCaspase3 as well as the apoptosis index of hemocytes were also decreased. These results collectively suggest that CgGLS-1 is the enzyme to synthesize Glu in oyster, which can modulate anti-bacterial immunity by regulating the secretion of pro-inflammatory cytokines CgIL17-5 and CgTNF-1, as well as hemocyte apoptosis. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Directory of Open Access Journals: DOAJ Articles Pacific Scientific Reports 11 1 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
topic |
Medicine R Science Q |
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Medicine R Science Q Yage Liang Meijia Li Zhaoqun Liu Yuanmei Li Lingling Wang Linsheng Song The glutaminase (CgGLS-1) mediates anti-bacterial immunity by prompting cytokine synthesis and hemocyte apoptosis in Pacific oyster Crassostrea gigas |
topic_facet |
Medicine R Science Q |
description |
Abstract Glutaminase, an amidohydrolase enzyme that hydrolyzes glutamine to glutamate, plays crucial roles in various immunomodulatory processes such as cell apoptosis, proliferation, migration, and secretion of cytokines. In the present study, a glutaminase homologue (designated as CgGLS-1) was identified from Pacific oyster Crassostrea gigas, whose open reading frame was of 1836 bp. CgGLS-1 exhibited high sequence identity with vertebrate kidney-type GLS, and closely clustered with their homologues from mollusc C. virginica. The enzyme activity of recombinant CgGLS-1 protein (rCgGLS-1) was estimated to be 1.705 U/mg. CgGLS-1 mRNA was constitutively expressed in all the tested tissues of oysters, with the highest expression level in hemocytes. CgGLS-1 mRNA expression in hemocytes was significantly up-regulated and peaked at 6 h (2.07-fold, p < 0.01) after lipopolysaccharide (LPS) stimulation. The CgGLS-1 protein was mainly distributed in the cytoplasm with a significant co-location with mitochondria in oyster hemocytes. The content of Glu in the oyster serum was significantly decreased after the inhibition of CgGLS-1 using specific inhibitor Bis-2- [5-(phenyl acetamido)-1,3,4-thiadiazol-2-yl] ethyl sulfide (BPTES), and the expression levels of CgmGluR6, CgAP-1, cytokines CgIL17-5 and CgTNF-1 were significantly decreased after BPTES and LPS stimulation. The transcripts of CgCaspase3 as well as the apoptosis index of hemocytes were also decreased. These results collectively suggest that CgGLS-1 is the enzyme to synthesize Glu in oyster, which can modulate anti-bacterial immunity by regulating the secretion of pro-inflammatory cytokines CgIL17-5 and CgTNF-1, as well as hemocyte apoptosis. |
format |
Article in Journal/Newspaper |
author |
Yage Liang Meijia Li Zhaoqun Liu Yuanmei Li Lingling Wang Linsheng Song |
author_facet |
Yage Liang Meijia Li Zhaoqun Liu Yuanmei Li Lingling Wang Linsheng Song |
author_sort |
Yage Liang |
title |
The glutaminase (CgGLS-1) mediates anti-bacterial immunity by prompting cytokine synthesis and hemocyte apoptosis in Pacific oyster Crassostrea gigas |
title_short |
The glutaminase (CgGLS-1) mediates anti-bacterial immunity by prompting cytokine synthesis and hemocyte apoptosis in Pacific oyster Crassostrea gigas |
title_full |
The glutaminase (CgGLS-1) mediates anti-bacterial immunity by prompting cytokine synthesis and hemocyte apoptosis in Pacific oyster Crassostrea gigas |
title_fullStr |
The glutaminase (CgGLS-1) mediates anti-bacterial immunity by prompting cytokine synthesis and hemocyte apoptosis in Pacific oyster Crassostrea gigas |
title_full_unstemmed |
The glutaminase (CgGLS-1) mediates anti-bacterial immunity by prompting cytokine synthesis and hemocyte apoptosis in Pacific oyster Crassostrea gigas |
title_sort |
glutaminase (cggls-1) mediates anti-bacterial immunity by prompting cytokine synthesis and hemocyte apoptosis in pacific oyster crassostrea gigas |
publisher |
Nature Portfolio |
publishDate |
2021 |
url |
https://doi.org/10.1038/s41598-020-80552-2 https://doaj.org/article/abf18a1f11f444348f7925d4c451342f |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Scientific Reports, Vol 11, Iss 1, Pp 1-14 (2021) |
op_relation |
https://doi.org/10.1038/s41598-020-80552-2 https://doaj.org/toc/2045-2322 doi:10.1038/s41598-020-80552-2 2045-2322 https://doaj.org/article/abf18a1f11f444348f7925d4c451342f |
op_doi |
https://doi.org/10.1038/s41598-020-80552-2 |
container_title |
Scientific Reports |
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11 |
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1 |
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1766394030331002880 |