Genetic diversity of Echinococcus spp. in wild carnivorous animals in Kazakhstan
Background and Aim: The study of Echinococcus infection among farm animals in Kazakhstan was carried out to monitor the invasion among livestock and map the data obtained. Unfortunately, there are only partial data on the study of echinococcosis among wild carnivores in Kazakhstan, which makes it di...
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ftdoajarticles:oai:doaj.org/article:abe654135f1748b7abea6115d8b00a9f 2023-05-15T15:51:12+02:00 Genetic diversity of Echinococcus spp. in wild carnivorous animals in Kazakhstan Rabiga Uakhit Ainura Smagulova Alfiya Syzdykova Sarsenbay Abdrakhmanov Vladimir Kiyan 2022-06-01T00:00:00Z https://doi.org/10.14202/vetworld.2022.1489-1496 https://doaj.org/article/abe654135f1748b7abea6115d8b00a9f EN eng Veterinary World http://www.veterinaryworld.org/Vol.15/June-2022/11.pdf https://doaj.org/toc/0972-8988 https://doaj.org/toc/2231-0916 doi:10.14202/vetworld.2022.1489-1496 0972-8988 2231-0916 https://doaj.org/article/abe654135f1748b7abea6115d8b00a9f Veterinary World, Vol 15, Iss 6, Pp 1489-1496 (2022) asian haplotype corsac echinococcosis phylogenetics red foxes wolf Animal culture SF1-1100 Veterinary medicine SF600-1100 article 2022 ftdoajarticles https://doi.org/10.14202/vetworld.2022.1489-1496 2022-12-31T02:31:23Z Background and Aim: The study of Echinococcus infection among farm animals in Kazakhstan was carried out to monitor the invasion among livestock and map the data obtained. Unfortunately, there are only partial data on the study of echinococcosis among wild carnivores in Kazakhstan, which makes it difficult to conduct a comparative analysis of the epidemiological situation among wild animals. The present study aimed to estimate the genetic diversity of Echinococcus spp. (Leuckart, 1863) in Kazakhstan based on sequence analysis of cytochrome c oxidase subunit 1 (cox1) and dehydrogenase subunit 1 (nad1) of worms isolated from wild carnivorous animals wolf (Canis lupus), red fox (Vulpes vulpes) and corsac (Vulpes corsac). Materials and Methods: DNA from parasite tissue was used as a template for the amplification of the two mitochondrial genes cox1 and nad1. Sequencing was performed according to the manual for the Seq Studio Genetic Analyzer. The multiple alignments of obtained sequences were performed using the ClustalW algorithm in Mega (v.11) software. Alignments were exported as a Nexus extension and used as input for TCS v1.21 for the identification of haplotypes. The phylogenetic analysis was constructed according to the neighbor-joining method using Mega (v.11) software. Results: Analysis of the extensiveness of echinococcosis invasion showed that 6.3% were wolves, 18.2% were corsacs, and 85% were foxes. In total, 159 adults of Echinococcus spp. from the three species of animals in different parts of Kazakhstan were analyzed, and 17 individual biological samples were successfully sequenced. Sequence analysis of cox1 and nad1 genes revealed two types of echinococcosis – Echinococcus granulosus in red foxes and wolves, and Echinococcus multilocularis in corsacs. Sequencing of a portion of the mitochondrial genome made it possible to determine seven haplotypes of the pathogen in the studied samples of E. granulosus. Molecular analysis of cox1 and nad1 genes of E. multilocularis revealed three new haplotypes, ... Article in Journal/Newspaper Canis lupus Directory of Open Access Journals: DOAJ Articles Veterinary World 1489 1496 |
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topic |
asian haplotype corsac echinococcosis phylogenetics red foxes wolf Animal culture SF1-1100 Veterinary medicine SF600-1100 |
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asian haplotype corsac echinococcosis phylogenetics red foxes wolf Animal culture SF1-1100 Veterinary medicine SF600-1100 Rabiga Uakhit Ainura Smagulova Alfiya Syzdykova Sarsenbay Abdrakhmanov Vladimir Kiyan Genetic diversity of Echinococcus spp. in wild carnivorous animals in Kazakhstan |
topic_facet |
asian haplotype corsac echinococcosis phylogenetics red foxes wolf Animal culture SF1-1100 Veterinary medicine SF600-1100 |
description |
Background and Aim: The study of Echinococcus infection among farm animals in Kazakhstan was carried out to monitor the invasion among livestock and map the data obtained. Unfortunately, there are only partial data on the study of echinococcosis among wild carnivores in Kazakhstan, which makes it difficult to conduct a comparative analysis of the epidemiological situation among wild animals. The present study aimed to estimate the genetic diversity of Echinococcus spp. (Leuckart, 1863) in Kazakhstan based on sequence analysis of cytochrome c oxidase subunit 1 (cox1) and dehydrogenase subunit 1 (nad1) of worms isolated from wild carnivorous animals wolf (Canis lupus), red fox (Vulpes vulpes) and corsac (Vulpes corsac). Materials and Methods: DNA from parasite tissue was used as a template for the amplification of the two mitochondrial genes cox1 and nad1. Sequencing was performed according to the manual for the Seq Studio Genetic Analyzer. The multiple alignments of obtained sequences were performed using the ClustalW algorithm in Mega (v.11) software. Alignments were exported as a Nexus extension and used as input for TCS v1.21 for the identification of haplotypes. The phylogenetic analysis was constructed according to the neighbor-joining method using Mega (v.11) software. Results: Analysis of the extensiveness of echinococcosis invasion showed that 6.3% were wolves, 18.2% were corsacs, and 85% were foxes. In total, 159 adults of Echinococcus spp. from the three species of animals in different parts of Kazakhstan were analyzed, and 17 individual biological samples were successfully sequenced. Sequence analysis of cox1 and nad1 genes revealed two types of echinococcosis – Echinococcus granulosus in red foxes and wolves, and Echinococcus multilocularis in corsacs. Sequencing of a portion of the mitochondrial genome made it possible to determine seven haplotypes of the pathogen in the studied samples of E. granulosus. Molecular analysis of cox1 and nad1 genes of E. multilocularis revealed three new haplotypes, ... |
format |
Article in Journal/Newspaper |
author |
Rabiga Uakhit Ainura Smagulova Alfiya Syzdykova Sarsenbay Abdrakhmanov Vladimir Kiyan |
author_facet |
Rabiga Uakhit Ainura Smagulova Alfiya Syzdykova Sarsenbay Abdrakhmanov Vladimir Kiyan |
author_sort |
Rabiga Uakhit |
title |
Genetic diversity of Echinococcus spp. in wild carnivorous animals in Kazakhstan |
title_short |
Genetic diversity of Echinococcus spp. in wild carnivorous animals in Kazakhstan |
title_full |
Genetic diversity of Echinococcus spp. in wild carnivorous animals in Kazakhstan |
title_fullStr |
Genetic diversity of Echinococcus spp. in wild carnivorous animals in Kazakhstan |
title_full_unstemmed |
Genetic diversity of Echinococcus spp. in wild carnivorous animals in Kazakhstan |
title_sort |
genetic diversity of echinococcus spp. in wild carnivorous animals in kazakhstan |
publisher |
Veterinary World |
publishDate |
2022 |
url |
https://doi.org/10.14202/vetworld.2022.1489-1496 https://doaj.org/article/abe654135f1748b7abea6115d8b00a9f |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Veterinary World, Vol 15, Iss 6, Pp 1489-1496 (2022) |
op_relation |
http://www.veterinaryworld.org/Vol.15/June-2022/11.pdf https://doaj.org/toc/0972-8988 https://doaj.org/toc/2231-0916 doi:10.14202/vetworld.2022.1489-1496 0972-8988 2231-0916 https://doaj.org/article/abe654135f1748b7abea6115d8b00a9f |
op_doi |
https://doi.org/10.14202/vetworld.2022.1489-1496 |
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Veterinary World |
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1489 |
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1496 |
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1766386276725948416 |