Inhibition of Primary Photosynthesis in Desiccating Antarctic Lichens Differing in Their Photobionts, Thallus Morphology, and Spectral Properties
Five macrolichens of different thallus morphology from Antarctica (King George Island) were used for this ecophysiological study. The effect of thallus desiccation on primary photosynthetic processes was examined. We investigated the lichens’ responses to the relative water content (RWC) in their th...
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ftdoajarticles:oai:doaj.org/article:abccf01ad14e4eda9cbd40f6fd99aae5 2023-05-15T13:54:21+02:00 Inhibition of Primary Photosynthesis in Desiccating Antarctic Lichens Differing in Their Photobionts, Thallus Morphology, and Spectral Properties Miloš Barták Josef Hájek Alla Orekhova Johana Villagra Catalina Marín Götz Palfner Angélica Casanova-Katny 2021-04-01T00:00:00Z https://doi.org/10.3390/microorganisms9040818 https://doaj.org/article/abccf01ad14e4eda9cbd40f6fd99aae5 EN eng MDPI AG https://www.mdpi.com/2076-2607/9/4/818 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms9040818 2076-2607 https://doaj.org/article/abccf01ad14e4eda9cbd40f6fd99aae5 Microorganisms, Vol 9, Iss 818, p 818 (2021) maritime antarctica King George Island lichen dehydration chlorophyll fluorescence stress tolerance Biology (General) QH301-705.5 article 2021 ftdoajarticles https://doi.org/10.3390/microorganisms9040818 2022-12-31T05:11:51Z Five macrolichens of different thallus morphology from Antarctica (King George Island) were used for this ecophysiological study. The effect of thallus desiccation on primary photosynthetic processes was examined. We investigated the lichens’ responses to the relative water content (RWC) in their thalli during the transition from a wet (RWC of 100%) to a dry state (RWC of 0%). The slow Kautsky kinetics of chlorophyll fluorescence (ChlF) that was recorded during controlled dehydration (RWC decreased from 100 to 0%) and supplemented with a quenching analysis revealed a polyphasic species-specific response of variable fluorescence. The changes in ChlF at a steady state (Fs), potential and effective quantum yields of photosystem II (F V /F M , Φ PSII ), and nonphotochemical quenching (NPQ) reflected a desiccation-induced inhibition of the photosynthetic processes. The dehydration-dependent fall in F V /F M and Φ PSII was species-specific, starting at an RWC range of 22–32%. The critical RWC for Φ PSII was below 5%. The changes indicated the involvement of protective mechanisms in the chloroplastic apparatus of lichen photobionts at RWCs of below 20%. In both the wet and dry states, the spectral reflectance curves (SRC) (wavelength 400–800 nm) and indices (NDVI, PRI) of the studied lichen species were measured. Black Himantormia lugubris showed no difference in the SRCs between wet and dry state. Other lichens showed a higher reflectance in the dry state compared to the wet state. The lichen morphology and anatomy data, together with the ChlF and spectral reflectance data, are discussed in relation to its potential for ecophysiological studies in Antarctic lichens. Article in Journal/Newspaper Antarc* Antarctic Antarctica King George Island Directory of Open Access Journals: DOAJ Articles Antarctic King George Island Microorganisms 9 4 818 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
maritime antarctica King George Island lichen dehydration chlorophyll fluorescence stress tolerance Biology (General) QH301-705.5 |
spellingShingle |
maritime antarctica King George Island lichen dehydration chlorophyll fluorescence stress tolerance Biology (General) QH301-705.5 Miloš Barták Josef Hájek Alla Orekhova Johana Villagra Catalina Marín Götz Palfner Angélica Casanova-Katny Inhibition of Primary Photosynthesis in Desiccating Antarctic Lichens Differing in Their Photobionts, Thallus Morphology, and Spectral Properties |
topic_facet |
maritime antarctica King George Island lichen dehydration chlorophyll fluorescence stress tolerance Biology (General) QH301-705.5 |
description |
Five macrolichens of different thallus morphology from Antarctica (King George Island) were used for this ecophysiological study. The effect of thallus desiccation on primary photosynthetic processes was examined. We investigated the lichens’ responses to the relative water content (RWC) in their thalli during the transition from a wet (RWC of 100%) to a dry state (RWC of 0%). The slow Kautsky kinetics of chlorophyll fluorescence (ChlF) that was recorded during controlled dehydration (RWC decreased from 100 to 0%) and supplemented with a quenching analysis revealed a polyphasic species-specific response of variable fluorescence. The changes in ChlF at a steady state (Fs), potential and effective quantum yields of photosystem II (F V /F M , Φ PSII ), and nonphotochemical quenching (NPQ) reflected a desiccation-induced inhibition of the photosynthetic processes. The dehydration-dependent fall in F V /F M and Φ PSII was species-specific, starting at an RWC range of 22–32%. The critical RWC for Φ PSII was below 5%. The changes indicated the involvement of protective mechanisms in the chloroplastic apparatus of lichen photobionts at RWCs of below 20%. In both the wet and dry states, the spectral reflectance curves (SRC) (wavelength 400–800 nm) and indices (NDVI, PRI) of the studied lichen species were measured. Black Himantormia lugubris showed no difference in the SRCs between wet and dry state. Other lichens showed a higher reflectance in the dry state compared to the wet state. The lichen morphology and anatomy data, together with the ChlF and spectral reflectance data, are discussed in relation to its potential for ecophysiological studies in Antarctic lichens. |
format |
Article in Journal/Newspaper |
author |
Miloš Barták Josef Hájek Alla Orekhova Johana Villagra Catalina Marín Götz Palfner Angélica Casanova-Katny |
author_facet |
Miloš Barták Josef Hájek Alla Orekhova Johana Villagra Catalina Marín Götz Palfner Angélica Casanova-Katny |
author_sort |
Miloš Barták |
title |
Inhibition of Primary Photosynthesis in Desiccating Antarctic Lichens Differing in Their Photobionts, Thallus Morphology, and Spectral Properties |
title_short |
Inhibition of Primary Photosynthesis in Desiccating Antarctic Lichens Differing in Their Photobionts, Thallus Morphology, and Spectral Properties |
title_full |
Inhibition of Primary Photosynthesis in Desiccating Antarctic Lichens Differing in Their Photobionts, Thallus Morphology, and Spectral Properties |
title_fullStr |
Inhibition of Primary Photosynthesis in Desiccating Antarctic Lichens Differing in Their Photobionts, Thallus Morphology, and Spectral Properties |
title_full_unstemmed |
Inhibition of Primary Photosynthesis in Desiccating Antarctic Lichens Differing in Their Photobionts, Thallus Morphology, and Spectral Properties |
title_sort |
inhibition of primary photosynthesis in desiccating antarctic lichens differing in their photobionts, thallus morphology, and spectral properties |
publisher |
MDPI AG |
publishDate |
2021 |
url |
https://doi.org/10.3390/microorganisms9040818 https://doaj.org/article/abccf01ad14e4eda9cbd40f6fd99aae5 |
geographic |
Antarctic King George Island |
geographic_facet |
Antarctic King George Island |
genre |
Antarc* Antarctic Antarctica King George Island |
genre_facet |
Antarc* Antarctic Antarctica King George Island |
op_source |
Microorganisms, Vol 9, Iss 818, p 818 (2021) |
op_relation |
https://www.mdpi.com/2076-2607/9/4/818 https://doaj.org/toc/2076-2607 doi:10.3390/microorganisms9040818 2076-2607 https://doaj.org/article/abccf01ad14e4eda9cbd40f6fd99aae5 |
op_doi |
https://doi.org/10.3390/microorganisms9040818 |
container_title |
Microorganisms |
container_volume |
9 |
container_issue |
4 |
container_start_page |
818 |
_version_ |
1766260069943476224 |