Dipeptide YA is Responsible for the Positive Effect of Oyster Hydrolysates on Alcohol Metabolism in Single Ethanol Binge Rodent Models
Accumulative alcohol hangovers cause liver damage through oxidative and inflammatory stress. Numerous antioxidant and anti-inflammatory reagents have been developed to reduce alcohol hangovers, but these reagents are still insignificant and have limitations in that they can cause liver toxicity. Oys...
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2020
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Online Access: | https://doi.org/10.3390/md18100512 https://doaj.org/article/abca2ebff7db4c2088a1214cfd9a8a2d |
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ftdoajarticles:oai:doaj.org/article:abca2ebff7db4c2088a1214cfd9a8a2d 2023-05-15T15:58:53+02:00 Dipeptide YA is Responsible for the Positive Effect of Oyster Hydrolysates on Alcohol Metabolism in Single Ethanol Binge Rodent Models Adrian S. Siregar Marie Merci Nyiramana Eun-Jin Kim Eui-Jung Shin Min Seok Woo Jin-Mok Kim Jung Hwan Kim Dong Kun Lee Jong Ryeal Hahm Hyun Joon Kim Chang-Woon Kim Nam-Gil Kim Si-Hyang Park Yeung Joon Choi Sang Soo Kang Seong-Geun Hong Jaehee Han Dawon Kang 2020-10-01T00:00:00Z https://doi.org/10.3390/md18100512 https://doaj.org/article/abca2ebff7db4c2088a1214cfd9a8a2d EN eng MDPI AG https://www.mdpi.com/1660-3397/18/10/512 https://doaj.org/toc/1660-3397 doi:10.3390/md18100512 1660-3397 https://doaj.org/article/abca2ebff7db4c2088a1214cfd9a8a2d Marine Drugs, Vol 18, Iss 512, p 512 (2020) alcohol inflammation liver injury oxidative stress Biology (General) QH301-705.5 article 2020 ftdoajarticles https://doi.org/10.3390/md18100512 2022-12-30T20:43:58Z Accumulative alcohol hangovers cause liver damage through oxidative and inflammatory stress. Numerous antioxidant and anti-inflammatory reagents have been developed to reduce alcohol hangovers, but these reagents are still insignificant and have limitations in that they can cause liver toxicity. Oyster hydrolysate (OH), another reagent that has antioxidant and anti-inflammatory activity, is a product extracted through an enzymatic hydrolysis process from oysters ( Crassostrea gigas ), which can be easily eaten in meals. This study was aimed at determining the effects of OH on alcohol metabolism, using a single high dose of ethanol (EtOH) administered to rodents, by monitoring alcohol metabolic enzymes, oxidative stress signals, and inflammatory mediators. The effect of tyrosine-alanine (YA) peptide, a main component of OH, on EtOH metabolism was also identified. In vitro experiments showed that OH pretreatment inhibited EtOH-induced cell death, oxidative stress, and inflammation in liver cells and macrophages. In vivo experiments showed that OH and YA pre-administration increased alcohol dehydrogenase, aldehyde dehydrogenase, and catalase activity in EtOH binge treatment. In addition, OH pre-administration alleviated CYP2E1 activity, ROS production, apoptotic signals, and inflammatory mediators in liver tissues. These results showed that OH and YA enhanced EtOH metabolism and had a protective effect against acute alcohol liver damage. Our findings offer new insights into a single high dose of EtOH drinking and suggest that OH and YA could be used as potential marine functional foods to prevent acute alcohol-induced liver damage. Article in Journal/Newspaper Crassostrea gigas Directory of Open Access Journals: DOAJ Articles Marine Drugs 18 10 512 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
alcohol inflammation liver injury oxidative stress Biology (General) QH301-705.5 |
spellingShingle |
alcohol inflammation liver injury oxidative stress Biology (General) QH301-705.5 Adrian S. Siregar Marie Merci Nyiramana Eun-Jin Kim Eui-Jung Shin Min Seok Woo Jin-Mok Kim Jung Hwan Kim Dong Kun Lee Jong Ryeal Hahm Hyun Joon Kim Chang-Woon Kim Nam-Gil Kim Si-Hyang Park Yeung Joon Choi Sang Soo Kang Seong-Geun Hong Jaehee Han Dawon Kang Dipeptide YA is Responsible for the Positive Effect of Oyster Hydrolysates on Alcohol Metabolism in Single Ethanol Binge Rodent Models |
topic_facet |
alcohol inflammation liver injury oxidative stress Biology (General) QH301-705.5 |
description |
Accumulative alcohol hangovers cause liver damage through oxidative and inflammatory stress. Numerous antioxidant and anti-inflammatory reagents have been developed to reduce alcohol hangovers, but these reagents are still insignificant and have limitations in that they can cause liver toxicity. Oyster hydrolysate (OH), another reagent that has antioxidant and anti-inflammatory activity, is a product extracted through an enzymatic hydrolysis process from oysters ( Crassostrea gigas ), which can be easily eaten in meals. This study was aimed at determining the effects of OH on alcohol metabolism, using a single high dose of ethanol (EtOH) administered to rodents, by monitoring alcohol metabolic enzymes, oxidative stress signals, and inflammatory mediators. The effect of tyrosine-alanine (YA) peptide, a main component of OH, on EtOH metabolism was also identified. In vitro experiments showed that OH pretreatment inhibited EtOH-induced cell death, oxidative stress, and inflammation in liver cells and macrophages. In vivo experiments showed that OH and YA pre-administration increased alcohol dehydrogenase, aldehyde dehydrogenase, and catalase activity in EtOH binge treatment. In addition, OH pre-administration alleviated CYP2E1 activity, ROS production, apoptotic signals, and inflammatory mediators in liver tissues. These results showed that OH and YA enhanced EtOH metabolism and had a protective effect against acute alcohol liver damage. Our findings offer new insights into a single high dose of EtOH drinking and suggest that OH and YA could be used as potential marine functional foods to prevent acute alcohol-induced liver damage. |
format |
Article in Journal/Newspaper |
author |
Adrian S. Siregar Marie Merci Nyiramana Eun-Jin Kim Eui-Jung Shin Min Seok Woo Jin-Mok Kim Jung Hwan Kim Dong Kun Lee Jong Ryeal Hahm Hyun Joon Kim Chang-Woon Kim Nam-Gil Kim Si-Hyang Park Yeung Joon Choi Sang Soo Kang Seong-Geun Hong Jaehee Han Dawon Kang |
author_facet |
Adrian S. Siregar Marie Merci Nyiramana Eun-Jin Kim Eui-Jung Shin Min Seok Woo Jin-Mok Kim Jung Hwan Kim Dong Kun Lee Jong Ryeal Hahm Hyun Joon Kim Chang-Woon Kim Nam-Gil Kim Si-Hyang Park Yeung Joon Choi Sang Soo Kang Seong-Geun Hong Jaehee Han Dawon Kang |
author_sort |
Adrian S. Siregar |
title |
Dipeptide YA is Responsible for the Positive Effect of Oyster Hydrolysates on Alcohol Metabolism in Single Ethanol Binge Rodent Models |
title_short |
Dipeptide YA is Responsible for the Positive Effect of Oyster Hydrolysates on Alcohol Metabolism in Single Ethanol Binge Rodent Models |
title_full |
Dipeptide YA is Responsible for the Positive Effect of Oyster Hydrolysates on Alcohol Metabolism in Single Ethanol Binge Rodent Models |
title_fullStr |
Dipeptide YA is Responsible for the Positive Effect of Oyster Hydrolysates on Alcohol Metabolism in Single Ethanol Binge Rodent Models |
title_full_unstemmed |
Dipeptide YA is Responsible for the Positive Effect of Oyster Hydrolysates on Alcohol Metabolism in Single Ethanol Binge Rodent Models |
title_sort |
dipeptide ya is responsible for the positive effect of oyster hydrolysates on alcohol metabolism in single ethanol binge rodent models |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/md18100512 https://doaj.org/article/abca2ebff7db4c2088a1214cfd9a8a2d |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Marine Drugs, Vol 18, Iss 512, p 512 (2020) |
op_relation |
https://www.mdpi.com/1660-3397/18/10/512 https://doaj.org/toc/1660-3397 doi:10.3390/md18100512 1660-3397 https://doaj.org/article/abca2ebff7db4c2088a1214cfd9a8a2d |
op_doi |
https://doi.org/10.3390/md18100512 |
container_title |
Marine Drugs |
container_volume |
18 |
container_issue |
10 |
container_start_page |
512 |
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1766394659329802240 |