Statistical Analysis of Community RNA Transcripts between Organic Carbon and Geogas-Fed Continental Deep Biosphere Groundwaters
ABSTRACT Life in water-filled bedrock fissures in the continental deep biosphere is broadly constrained by energy and nutrient availability. Although these communities are alive, robust studies comparing active populations and metabolic processes across deep aquifers are lacking. This study analyzed...
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ftdoajarticles:oai:doaj.org/article:abc5f52717b346efbbd540ad540260f0 2023-05-15T16:13:09+02:00 Statistical Analysis of Community RNA Transcripts between Organic Carbon and Geogas-Fed Continental Deep Biosphere Groundwaters Margarita Lopez-Fernandez Elias Broman Domenico Simone Stefan Bertilsson Mark Dopson 2019-08-01T00:00:00Z https://doi.org/10.1128/mBio.01470-19 https://doaj.org/article/abc5f52717b346efbbd540ad540260f0 EN eng American Society for Microbiology https://journals.asm.org/doi/10.1128/mBio.01470-19 https://doaj.org/toc/2150-7511 doi:10.1128/mBio.01470-19 2150-7511 https://doaj.org/article/abc5f52717b346efbbd540ad540260f0 mBio, Vol 10, Iss 4 (2019) deep biosphere groundwaters metatranscriptomes protein-coding RNA rRNA Microbiology QR1-502 article 2019 ftdoajarticles https://doi.org/10.1128/mBio.01470-19 2022-12-31T13:57:41Z ABSTRACT Life in water-filled bedrock fissures in the continental deep biosphere is broadly constrained by energy and nutrient availability. Although these communities are alive, robust studies comparing active populations and metabolic processes across deep aquifers are lacking. This study analyzed three oligotrophic Fennoscandian Shield groundwaters, two “modern marine” waters that are replenished with organic carbon from the Baltic Sea and are likely less than 20 years old (171.3 and 415.4 m below sea level) and an extremely oligotrophic “thoroughly mixed” water (448.8 m below sea level) of unknown age that is composed of very old saline and marine waters. Cells were captured either using a sampling device that rapidly fixed RNA under in situ conditions or by filtering flowing groundwater over an extended period before fixation. Comparison of metatranscriptomes between the methods showed statistically similar transcript profiles for the respective water types, and they were analyzed as biological replicates. Study of the small subunit (SSU) rRNA confirmed active populations from all three domains of life, with many potentially novel unclassified populations present. Statistically supported differences between communities included heterotrophic sulfate-reducing bacteria in the modern marine water at 171.3 m below sea level that has a higher organic carbon content than do largely autotrophic populations in the H2- and CO2-fed thoroughly mixed water. While this modern marine water had signatures of methanogenesis, syntrophic populations were predominantly in the thoroughly mixed water. The study provides a first statistical evaluation of differences in the active microbial communities in groundwaters differentially fed by organic carbon or “geogases.” IMPORTANCE Despite being separated from the photosynthesis-driven surface by both distance and time, the deep biosphere is an important driver for the earth’s carbon and energy cycles. However, due to the difficulties in gaining access and low cell numbers, robust ... Article in Journal/Newspaper Fennoscandian Directory of Open Access Journals: DOAJ Articles mBio 10 4 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
deep biosphere groundwaters metatranscriptomes protein-coding RNA rRNA Microbiology QR1-502 |
spellingShingle |
deep biosphere groundwaters metatranscriptomes protein-coding RNA rRNA Microbiology QR1-502 Margarita Lopez-Fernandez Elias Broman Domenico Simone Stefan Bertilsson Mark Dopson Statistical Analysis of Community RNA Transcripts between Organic Carbon and Geogas-Fed Continental Deep Biosphere Groundwaters |
topic_facet |
deep biosphere groundwaters metatranscriptomes protein-coding RNA rRNA Microbiology QR1-502 |
description |
ABSTRACT Life in water-filled bedrock fissures in the continental deep biosphere is broadly constrained by energy and nutrient availability. Although these communities are alive, robust studies comparing active populations and metabolic processes across deep aquifers are lacking. This study analyzed three oligotrophic Fennoscandian Shield groundwaters, two “modern marine” waters that are replenished with organic carbon from the Baltic Sea and are likely less than 20 years old (171.3 and 415.4 m below sea level) and an extremely oligotrophic “thoroughly mixed” water (448.8 m below sea level) of unknown age that is composed of very old saline and marine waters. Cells were captured either using a sampling device that rapidly fixed RNA under in situ conditions or by filtering flowing groundwater over an extended period before fixation. Comparison of metatranscriptomes between the methods showed statistically similar transcript profiles for the respective water types, and they were analyzed as biological replicates. Study of the small subunit (SSU) rRNA confirmed active populations from all three domains of life, with many potentially novel unclassified populations present. Statistically supported differences between communities included heterotrophic sulfate-reducing bacteria in the modern marine water at 171.3 m below sea level that has a higher organic carbon content than do largely autotrophic populations in the H2- and CO2-fed thoroughly mixed water. While this modern marine water had signatures of methanogenesis, syntrophic populations were predominantly in the thoroughly mixed water. The study provides a first statistical evaluation of differences in the active microbial communities in groundwaters differentially fed by organic carbon or “geogases.” IMPORTANCE Despite being separated from the photosynthesis-driven surface by both distance and time, the deep biosphere is an important driver for the earth’s carbon and energy cycles. However, due to the difficulties in gaining access and low cell numbers, robust ... |
format |
Article in Journal/Newspaper |
author |
Margarita Lopez-Fernandez Elias Broman Domenico Simone Stefan Bertilsson Mark Dopson |
author_facet |
Margarita Lopez-Fernandez Elias Broman Domenico Simone Stefan Bertilsson Mark Dopson |
author_sort |
Margarita Lopez-Fernandez |
title |
Statistical Analysis of Community RNA Transcripts between Organic Carbon and Geogas-Fed Continental Deep Biosphere Groundwaters |
title_short |
Statistical Analysis of Community RNA Transcripts between Organic Carbon and Geogas-Fed Continental Deep Biosphere Groundwaters |
title_full |
Statistical Analysis of Community RNA Transcripts between Organic Carbon and Geogas-Fed Continental Deep Biosphere Groundwaters |
title_fullStr |
Statistical Analysis of Community RNA Transcripts between Organic Carbon and Geogas-Fed Continental Deep Biosphere Groundwaters |
title_full_unstemmed |
Statistical Analysis of Community RNA Transcripts between Organic Carbon and Geogas-Fed Continental Deep Biosphere Groundwaters |
title_sort |
statistical analysis of community rna transcripts between organic carbon and geogas-fed continental deep biosphere groundwaters |
publisher |
American Society for Microbiology |
publishDate |
2019 |
url |
https://doi.org/10.1128/mBio.01470-19 https://doaj.org/article/abc5f52717b346efbbd540ad540260f0 |
genre |
Fennoscandian |
genre_facet |
Fennoscandian |
op_source |
mBio, Vol 10, Iss 4 (2019) |
op_relation |
https://journals.asm.org/doi/10.1128/mBio.01470-19 https://doaj.org/toc/2150-7511 doi:10.1128/mBio.01470-19 2150-7511 https://doaj.org/article/abc5f52717b346efbbd540ad540260f0 |
op_doi |
https://doi.org/10.1128/mBio.01470-19 |
container_title |
mBio |
container_volume |
10 |
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4 |
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1765998764223365120 |