Airborne Transmission of Melioidosis to Humans from Environmental Aerosols Contaminated with B. pseudomallei.
Melioidosis results from an infection with the soil-borne pathogen Burkholderia pseudomallei, and cases of melioidosis usually cluster after rains or a typhoon. In an endemic area of Taiwan, B. pseudomallei is primarily geographically distributed in cropped fields in the northwest of this area, wher...
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ftdoajarticles:oai:doaj.org/article:ab9fd412cfcc4a4f85d3dee1934f2689 2023-05-15T15:11:13+02:00 Airborne Transmission of Melioidosis to Humans from Environmental Aerosols Contaminated with B. pseudomallei. Pei-Shih Chen Yao-Shen Chen Hsi-Hsun Lin Pei-Ju Liu Wei-Fan Ni Pei-Tan Hsueh Shih-Hsiung Liang Chialin Chen Ya-Lei Chen 2015-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0003834 https://doaj.org/article/ab9fd412cfcc4a4f85d3dee1934f2689 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4462588?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003834 https://doaj.org/article/ab9fd412cfcc4a4f85d3dee1934f2689 PLoS Neglected Tropical Diseases, Vol 9, Iss 6, p e0003834 (2015) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2015 ftdoajarticles https://doi.org/10.1371/journal.pntd.0003834 2022-12-31T12:17:47Z Melioidosis results from an infection with the soil-borne pathogen Burkholderia pseudomallei, and cases of melioidosis usually cluster after rains or a typhoon. In an endemic area of Taiwan, B. pseudomallei is primarily geographically distributed in cropped fields in the northwest of this area, whereas melioidosis cases are distributed in a densely populated district in the southeast. We hypothesized that contaminated cropped fields generated aerosols contaminated with B. pseudomallei, which were carried by a northwesterly wind to the densely populated southeastern district. We collected soil and aerosol samples from a 72 km2 area of land, including the melioidosis-clustered area and its surroundings. Aerosols that contained B. pseudomallei-specific TTSS (type III secretion system) ORF2 DNA were well distributed in the endemic area but were rare in the surrounding areas during the rainy season. The concentration of this specific DNA in aerosols was positively correlated with the incidence of melioidosis and the appearance of a northwesterly wind. Moreover, the isolation rate in the superficial layers of the contaminated cropped field in the northwest was correlated with PCR positivity for aerosols collected from the southeast over a 2-year period. According to pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) analyses, PFGE Type Ia (ST58) was the predominant pattern linking the molecular association among soil, aerosol and human isolates. Thus, the airborne transmission of melioidosis moves from the contaminated soil to aerosols and/or to humans in this endemic area. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 9 6 e0003834 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Pei-Shih Chen Yao-Shen Chen Hsi-Hsun Lin Pei-Ju Liu Wei-Fan Ni Pei-Tan Hsueh Shih-Hsiung Liang Chialin Chen Ya-Lei Chen Airborne Transmission of Melioidosis to Humans from Environmental Aerosols Contaminated with B. pseudomallei. |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Melioidosis results from an infection with the soil-borne pathogen Burkholderia pseudomallei, and cases of melioidosis usually cluster after rains or a typhoon. In an endemic area of Taiwan, B. pseudomallei is primarily geographically distributed in cropped fields in the northwest of this area, whereas melioidosis cases are distributed in a densely populated district in the southeast. We hypothesized that contaminated cropped fields generated aerosols contaminated with B. pseudomallei, which were carried by a northwesterly wind to the densely populated southeastern district. We collected soil and aerosol samples from a 72 km2 area of land, including the melioidosis-clustered area and its surroundings. Aerosols that contained B. pseudomallei-specific TTSS (type III secretion system) ORF2 DNA were well distributed in the endemic area but were rare in the surrounding areas during the rainy season. The concentration of this specific DNA in aerosols was positively correlated with the incidence of melioidosis and the appearance of a northwesterly wind. Moreover, the isolation rate in the superficial layers of the contaminated cropped field in the northwest was correlated with PCR positivity for aerosols collected from the southeast over a 2-year period. According to pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) analyses, PFGE Type Ia (ST58) was the predominant pattern linking the molecular association among soil, aerosol and human isolates. Thus, the airborne transmission of melioidosis moves from the contaminated soil to aerosols and/or to humans in this endemic area. |
format |
Article in Journal/Newspaper |
author |
Pei-Shih Chen Yao-Shen Chen Hsi-Hsun Lin Pei-Ju Liu Wei-Fan Ni Pei-Tan Hsueh Shih-Hsiung Liang Chialin Chen Ya-Lei Chen |
author_facet |
Pei-Shih Chen Yao-Shen Chen Hsi-Hsun Lin Pei-Ju Liu Wei-Fan Ni Pei-Tan Hsueh Shih-Hsiung Liang Chialin Chen Ya-Lei Chen |
author_sort |
Pei-Shih Chen |
title |
Airborne Transmission of Melioidosis to Humans from Environmental Aerosols Contaminated with B. pseudomallei. |
title_short |
Airborne Transmission of Melioidosis to Humans from Environmental Aerosols Contaminated with B. pseudomallei. |
title_full |
Airborne Transmission of Melioidosis to Humans from Environmental Aerosols Contaminated with B. pseudomallei. |
title_fullStr |
Airborne Transmission of Melioidosis to Humans from Environmental Aerosols Contaminated with B. pseudomallei. |
title_full_unstemmed |
Airborne Transmission of Melioidosis to Humans from Environmental Aerosols Contaminated with B. pseudomallei. |
title_sort |
airborne transmission of melioidosis to humans from environmental aerosols contaminated with b. pseudomallei. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pntd.0003834 https://doaj.org/article/ab9fd412cfcc4a4f85d3dee1934f2689 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 9, Iss 6, p e0003834 (2015) |
op_relation |
http://europepmc.org/articles/PMC4462588?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003834 https://doaj.org/article/ab9fd412cfcc4a4f85d3dee1934f2689 |
op_doi |
https://doi.org/10.1371/journal.pntd.0003834 |
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PLOS Neglected Tropical Diseases |
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9 |
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6 |
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e0003834 |
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