2La chromosomal inversion enhances thermal tolerance of Anopheles gambiae larvae
Abstract Background The mosquito Anopheles gambiae is broadly distributed throughout sub-Saharan Africa and this contributes to making it the most efficient vector of malaria on the continent. The pervasiveness of this species is hypothesized to originate in local adaptations facilitated by inversio...
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ftdoajarticles:oai:doaj.org/article:ab9d131355ac4f108be8aad487557179 2023-05-15T15:14:59+02:00 2La chromosomal inversion enhances thermal tolerance of Anopheles gambiae larvae Costantini Carlo Gray Emilie M Rocca Kyle AC Besansky Nora J 2009-07-01T00:00:00Z https://doi.org/10.1186/1475-2875-8-147 https://doaj.org/article/ab9d131355ac4f108be8aad487557179 EN eng BMC http://www.malariajournal.com/content/8/1/147 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-8-147 1475-2875 https://doaj.org/article/ab9d131355ac4f108be8aad487557179 Malaria Journal, Vol 8, Iss 1, p 147 (2009) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2009 ftdoajarticles https://doi.org/10.1186/1475-2875-8-147 2022-12-31T11:57:02Z Abstract Background The mosquito Anopheles gambiae is broadly distributed throughout sub-Saharan Africa and this contributes to making it the most efficient vector of malaria on the continent. The pervasiveness of this species is hypothesized to originate in local adaptations facilitated by inversion polymorphisms. One inversion, named 2La, is strongly associated with aridity clines in West and Central Africa: while 2La is fixed in arid savannas, the 2L+ a arrangement is predominantly found in the rainforest. Ability to survive high temperature exposure is an essential component of aridity tolerance, particularly in immature stages that are restricted to shallow puddles. Toward deciphering the role of the 2La inversion in local adaptation, the present investigation focused on variation in larval and pupal thermo-tolerance in two populations dissimilar solely in 2La arrangement. Methods A laboratory colony of A. gambiae that is polymorphic for 2La but standard for all other known inversions was used to create 2 homokaryotypic populations (2L+ a and 2La). The survival of 4 th instar larvae and pupae from both populations was then tested following exposure to thermal stress with and without prior heat hardening. Results Larvae responded identically to a 40°C heat stress, with about 50% of larvae dying after 1.5–2 h and few larvae surviving a 3 h stress. When heat hardened prior to the thermal stress, thermo-tolerance of both larval populations increased, with 2La 24 h survival significantly exceeding that of 2L+ a . Pupae were generally more thermo-tolerant than larvae, although 2La pupae were less so than 2L+ a . Heat hardening had no positive effect on pupal thermo-tolerance. Conclusion The increased thermo-tolerance observed in 2La larvae following heat hardening suggests higher responsiveness (i.e., thermal sensitivity) of the inverted karyotype. By responding more drastically to the heat shock, 2La larvae are better equipped to resist the potentially lethal temperatures that occur in arid habitats. The lower ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 8 1 147 |
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English |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Costantini Carlo Gray Emilie M Rocca Kyle AC Besansky Nora J 2La chromosomal inversion enhances thermal tolerance of Anopheles gambiae larvae |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background The mosquito Anopheles gambiae is broadly distributed throughout sub-Saharan Africa and this contributes to making it the most efficient vector of malaria on the continent. The pervasiveness of this species is hypothesized to originate in local adaptations facilitated by inversion polymorphisms. One inversion, named 2La, is strongly associated with aridity clines in West and Central Africa: while 2La is fixed in arid savannas, the 2L+ a arrangement is predominantly found in the rainforest. Ability to survive high temperature exposure is an essential component of aridity tolerance, particularly in immature stages that are restricted to shallow puddles. Toward deciphering the role of the 2La inversion in local adaptation, the present investigation focused on variation in larval and pupal thermo-tolerance in two populations dissimilar solely in 2La arrangement. Methods A laboratory colony of A. gambiae that is polymorphic for 2La but standard for all other known inversions was used to create 2 homokaryotypic populations (2L+ a and 2La). The survival of 4 th instar larvae and pupae from both populations was then tested following exposure to thermal stress with and without prior heat hardening. Results Larvae responded identically to a 40°C heat stress, with about 50% of larvae dying after 1.5–2 h and few larvae surviving a 3 h stress. When heat hardened prior to the thermal stress, thermo-tolerance of both larval populations increased, with 2La 24 h survival significantly exceeding that of 2L+ a . Pupae were generally more thermo-tolerant than larvae, although 2La pupae were less so than 2L+ a . Heat hardening had no positive effect on pupal thermo-tolerance. Conclusion The increased thermo-tolerance observed in 2La larvae following heat hardening suggests higher responsiveness (i.e., thermal sensitivity) of the inverted karyotype. By responding more drastically to the heat shock, 2La larvae are better equipped to resist the potentially lethal temperatures that occur in arid habitats. The lower ... |
format |
Article in Journal/Newspaper |
author |
Costantini Carlo Gray Emilie M Rocca Kyle AC Besansky Nora J |
author_facet |
Costantini Carlo Gray Emilie M Rocca Kyle AC Besansky Nora J |
author_sort |
Costantini Carlo |
title |
2La chromosomal inversion enhances thermal tolerance of Anopheles gambiae larvae |
title_short |
2La chromosomal inversion enhances thermal tolerance of Anopheles gambiae larvae |
title_full |
2La chromosomal inversion enhances thermal tolerance of Anopheles gambiae larvae |
title_fullStr |
2La chromosomal inversion enhances thermal tolerance of Anopheles gambiae larvae |
title_full_unstemmed |
2La chromosomal inversion enhances thermal tolerance of Anopheles gambiae larvae |
title_sort |
2la chromosomal inversion enhances thermal tolerance of anopheles gambiae larvae |
publisher |
BMC |
publishDate |
2009 |
url |
https://doi.org/10.1186/1475-2875-8-147 https://doaj.org/article/ab9d131355ac4f108be8aad487557179 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 8, Iss 1, p 147 (2009) |
op_relation |
http://www.malariajournal.com/content/8/1/147 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-8-147 1475-2875 https://doaj.org/article/ab9d131355ac4f108be8aad487557179 |
op_doi |
https://doi.org/10.1186/1475-2875-8-147 |
container_title |
Malaria Journal |
container_volume |
8 |
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1 |
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147 |
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