Amazonia Seasons Have an Influence in the Composition of Bacterial Gut Microbiota of Mangrove Oysters (Crassostrea gasar)
The mangrove oysters (Crassostrea gasar) are molluscs native to the Amazonia region and their exploration and farming has increased considerably in recent years. These animals are farmed on beds built in the rivers of the Amazonia estuaries and, therefore, the composition of their microbiome should...
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ftdoajarticles:oai:doaj.org/article:ab74b3ee90c14e2fa9adea6093812e70 2023-05-15T15:58:51+02:00 Amazonia Seasons Have an Influence in the Composition of Bacterial Gut Microbiota of Mangrove Oysters (Crassostrea gasar) Marcos Vinícius Reis Conceição Sávio Souza Costa Ana Paula Schaan Ândrea Kely Campos Ribeiro-dos-Santos Artur Silva Diego Assis das Graças Maria Paula Cruz Schneider Rafael Azevedo Baraúna 2021-02-01T00:00:00Z https://doi.org/10.3389/fgene.2020.602608 https://doaj.org/article/ab74b3ee90c14e2fa9adea6093812e70 EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fgene.2020.602608/full https://doaj.org/toc/1664-8021 1664-8021 doi:10.3389/fgene.2020.602608 https://doaj.org/article/ab74b3ee90c14e2fa9adea6093812e70 Frontiers in Genetics, Vol 11 (2021) mangrove oyster oyster oyster microbiota Amazonia Crassostrea gasar Genetics QH426-470 article 2021 ftdoajarticles https://doi.org/10.3389/fgene.2020.602608 2022-12-31T13:27:44Z The mangrove oysters (Crassostrea gasar) are molluscs native to the Amazonia region and their exploration and farming has increased considerably in recent years. These animals are farmed on beds built in the rivers of the Amazonia estuaries and, therefore, the composition of their microbiome should be directly influenced by environmental conditions. Our work aimed to evaluate the changes in bacterial composition of oyster's microbiota at two different seasons (rainy and dry). For this purpose, we amplified and sequenced the V3-V4 regions of the 16S rRNA gene. Sequencing was performed on the Illumina MiSeq platform. According to the rarefaction curve, the sampling effort was sufficient to describe the bacterial diversity in the samples. Alpha-diversity indexes showed that the bacterial microbiota of oysters is richer during the rainy season. This richness is possibly associated with the diversity at lower taxonomic levels, since the relative abundance of bacterial phyla in the two seasons remained relatively constant. The main phyla found include Firmicutes, Bacteroidetes, Actinobacteria, and Proteobacteria. Similar results were found for the species Crassostrea gigas, Crassostrea sikamea, and Crassostrea corteziensis. Beta-diversity analysis showed that the bacterial composition of oyster's gut microbiota was quite different in the two seasons. Our data demonstrate the close relationship between the environment and the microbiome of these molluscs, reinforcing the need for conservation and sustainable management of estuaries in the Amazonia. Article in Journal/Newspaper Crassostrea gigas Directory of Open Access Journals: DOAJ Articles Frontiers in Genetics 11 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
mangrove oyster oyster oyster microbiota Amazonia Crassostrea gasar Genetics QH426-470 |
spellingShingle |
mangrove oyster oyster oyster microbiota Amazonia Crassostrea gasar Genetics QH426-470 Marcos Vinícius Reis Conceição Sávio Souza Costa Ana Paula Schaan Ândrea Kely Campos Ribeiro-dos-Santos Artur Silva Diego Assis das Graças Maria Paula Cruz Schneider Rafael Azevedo Baraúna Amazonia Seasons Have an Influence in the Composition of Bacterial Gut Microbiota of Mangrove Oysters (Crassostrea gasar) |
topic_facet |
mangrove oyster oyster oyster microbiota Amazonia Crassostrea gasar Genetics QH426-470 |
description |
The mangrove oysters (Crassostrea gasar) are molluscs native to the Amazonia region and their exploration and farming has increased considerably in recent years. These animals are farmed on beds built in the rivers of the Amazonia estuaries and, therefore, the composition of their microbiome should be directly influenced by environmental conditions. Our work aimed to evaluate the changes in bacterial composition of oyster's microbiota at two different seasons (rainy and dry). For this purpose, we amplified and sequenced the V3-V4 regions of the 16S rRNA gene. Sequencing was performed on the Illumina MiSeq platform. According to the rarefaction curve, the sampling effort was sufficient to describe the bacterial diversity in the samples. Alpha-diversity indexes showed that the bacterial microbiota of oysters is richer during the rainy season. This richness is possibly associated with the diversity at lower taxonomic levels, since the relative abundance of bacterial phyla in the two seasons remained relatively constant. The main phyla found include Firmicutes, Bacteroidetes, Actinobacteria, and Proteobacteria. Similar results were found for the species Crassostrea gigas, Crassostrea sikamea, and Crassostrea corteziensis. Beta-diversity analysis showed that the bacterial composition of oyster's gut microbiota was quite different in the two seasons. Our data demonstrate the close relationship between the environment and the microbiome of these molluscs, reinforcing the need for conservation and sustainable management of estuaries in the Amazonia. |
format |
Article in Journal/Newspaper |
author |
Marcos Vinícius Reis Conceição Sávio Souza Costa Ana Paula Schaan Ândrea Kely Campos Ribeiro-dos-Santos Artur Silva Diego Assis das Graças Maria Paula Cruz Schneider Rafael Azevedo Baraúna |
author_facet |
Marcos Vinícius Reis Conceição Sávio Souza Costa Ana Paula Schaan Ândrea Kely Campos Ribeiro-dos-Santos Artur Silva Diego Assis das Graças Maria Paula Cruz Schneider Rafael Azevedo Baraúna |
author_sort |
Marcos Vinícius Reis Conceição |
title |
Amazonia Seasons Have an Influence in the Composition of Bacterial Gut Microbiota of Mangrove Oysters (Crassostrea gasar) |
title_short |
Amazonia Seasons Have an Influence in the Composition of Bacterial Gut Microbiota of Mangrove Oysters (Crassostrea gasar) |
title_full |
Amazonia Seasons Have an Influence in the Composition of Bacterial Gut Microbiota of Mangrove Oysters (Crassostrea gasar) |
title_fullStr |
Amazonia Seasons Have an Influence in the Composition of Bacterial Gut Microbiota of Mangrove Oysters (Crassostrea gasar) |
title_full_unstemmed |
Amazonia Seasons Have an Influence in the Composition of Bacterial Gut Microbiota of Mangrove Oysters (Crassostrea gasar) |
title_sort |
amazonia seasons have an influence in the composition of bacterial gut microbiota of mangrove oysters (crassostrea gasar) |
publisher |
Frontiers Media S.A. |
publishDate |
2021 |
url |
https://doi.org/10.3389/fgene.2020.602608 https://doaj.org/article/ab74b3ee90c14e2fa9adea6093812e70 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Frontiers in Genetics, Vol 11 (2021) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fgene.2020.602608/full https://doaj.org/toc/1664-8021 1664-8021 doi:10.3389/fgene.2020.602608 https://doaj.org/article/ab74b3ee90c14e2fa9adea6093812e70 |
op_doi |
https://doi.org/10.3389/fgene.2020.602608 |
container_title |
Frontiers in Genetics |
container_volume |
11 |
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1766394619737669632 |