Comparison on the performance of Leishmania major-like and Leishmania braziliensis braziliensis as antigen for new world leishmaniasis IgG-immunofluorescence test Comparação do desempenho de antígenos de Leishmania major-like e Leishmania braziliensis braziliensis no teste de imunofluorescência-IgG para leishmaniose mucocutânea

• Published in: Revista do Instituto de Medicina Tropical de São Paulo
• Main Authors: Maria Carolina Soares Guimarães, Beatriz J. Celeste, Edelma María Corrales L., Carlos M.F. Antunes
• Format: Article in Journal/Newspaper
• Language: English
• Published: Universidade de São Paulo (USP) 1991
• Subjects: L. major-like; braziliensis braziliensis; IgG; immunofluorescence test; Arctic medicine. Tropical medicine; RC955-962; Infectious and parasitic diseases; RC109-216; Arctic; The ''Y''
• Online Access: https://doi.org/10.1590/S0036-46651991000600012; https://doaj.org/article/ab60cfb0bdfe496e9dbc4cd9c61b3d40

The performance of an antigen of L. major-like promastigotes for the serological diagnosis of mucocutaneous leishmaniasis in the IgG-immunofluorescent test was compared to that of an antigen of L. braziliensis braziliensis. Each antigen was used to test two hundred and twenty-four sera of etiologies such as mucocutaneous leishmaniasis, deep mycoses, toxoplasmosis, malaria, Chagas' disease, visceral leishmaniasis, anti-nuclear factor, schistosomaiasis, rheumatoid factor and normal controls. Agreement between responses to each antigen was high: 77.2% of leishmaniases sera agreed on a positive or a negative result to both antigens and 91.1 % of control sera. Cross reactivity was restricted to Chagas' disease sera, visceral leishmaniasis, anti-nuclear factor and paracoccidiodomycosis. The quantitative response of leishmaniasis and Chagas' disease sera to both antigens was evaluated by a linear regression; although the y-intercept and the slope were different for each antigen, neither was better than the other in the disclosure of anti-Leishmania antibodies. In the case of Chagas' disease sera the L. major-like antigen was better than L. b. braziliensis' to disclose cross-reacting antibodies. O desempenho de um antígeno de promastigotas de L. major-like para o diagnóstico sorológico de leishmaniose mucocutânea pelo teste de imunofluorescência-IgG foi comparado com o desempenho de um antígeno de L. braziliensis braziliensis. Cada antígeno foi usado para testar 224 soros de etiologias como leishmaniose mucocutânea, micoses profundas, toxoplasmose, malária, doença de Chagas, leishmaniose visceral, fator anti-núcleo, esquistossomose mansônica, fator reumatóide e controles normais. A concordância entre as respostas a cada antígeno foi grande: 77,2% dos soros de leishmaniose mostraram resultado positivo para ambos os antígenos ou negativo, assim como 91,1% dos soros controle negativos. Reações cruzadas ficaram restritas à doença de Chagas, leishmaniose visceral, fator anti-núcleo e paracoccidioidomicose. A resposta ...