Seroprevalence of Kaposi's sarcoma-associated herpesvirus in various populations in Cuba Seroprevalencia del herpesvirus asociado con el sarcoma de Kaposi en diversas poblaciones en Cuba

OBJECTIVE: Little is known about the prevalence and distribution of Kaposi's sarcoma-associated herpesvirus (KSHV) infection in the Caribbean. The aim of this study was to determine rates of KSHV seropositivity in various populations in Cuba. METHODS: During the years 1998 to 2002 we screened s...

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Main Authors: Vivian Kourí, Sybil M. Eng, María E. Rodríguez, Sonia Resik, Odalys Orraca, Patrick S. Moore, Yuan Chang
Format: Article in Journal/Newspaper
Language:English
Spanish
Portuguese
Published: Pan American Health Organization 2004
Subjects:
HIV
R
Online Access:https://doaj.org/article/aaaf011e296a4419aff0b1f13259d501
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spelling ftdoajarticles:oai:doaj.org/article:aaaf011e296a4419aff0b1f13259d501 2023-05-15T15:17:09+02:00 Seroprevalence of Kaposi's sarcoma-associated herpesvirus in various populations in Cuba Seroprevalencia del herpesvirus asociado con el sarcoma de Kaposi en diversas poblaciones en Cuba Vivian Kourí Sybil M. Eng María E. Rodríguez Sonia Resik Odalys Orraca Patrick S. Moore Yuan Chang 2004-05-01T00:00:00Z https://doaj.org/article/aaaf011e296a4419aff0b1f13259d501 EN ES PT eng spa por Pan American Health Organization http://www.scielosp.org/scielo.php?script=sci_arttext&pid=S1020-49892004000500006 https://doaj.org/toc/1020-4989 https://doaj.org/toc/1680-5348 1020-4989 1680-5348 https://doaj.org/article/aaaf011e296a4419aff0b1f13259d501 Revista Panamericana de Salud Pública, Vol 15, Iss 5, Pp 320-325 (2004) Herpesvirus 8 sarcoma HIV acquired immunodeficiency syndrome risk factors seroepidemiologic studies Cuba Medicine R Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2004 ftdoajarticles 2022-12-30T20:50:05Z OBJECTIVE: Little is known about the prevalence and distribution of Kaposi's sarcoma-associated herpesvirus (KSHV) infection in the Caribbean. The aim of this study was to determine rates of KSHV seropositivity in various populations in Cuba. METHODS: During the years 1998 to 2002 we screened serum samples from 410 subjects in Cuba. Serologic screening for KSHV antibodies was a two-step process using (1) indirect immunofluorescence assay (IFA) specifically reactive to the KSHV latency-associated nuclear antigen (LANA) encoded by open reading frame 73 (ORF73), and (2) confirmatory immunoblot using recombinant KSHV ORF65.2, a lytically expressed, 20-kilodalton protein as the target antigen. Five different populations were studied: (1) 45 AIDS patients with Kaposi's sarcoma (AIDS-KS), (2) 154 HIV-1-infected patients without clinical evidence of KS, (3) 171 HIV-negative blood donors, (4) 27 consecutive kidney transplant recipients, who were HIV-negative, and (5) 13 contacts (sexual contacts or relatives) of the AIDS-KS-affected patients. RESULTS: Among the 45 AIDS-KS subjects, 35 of them (77.8%) were KSHV-seropositive. Thirty-two of the 154 HIV-positive patients without KS (20.8% of them) were KSHV-seropositive, and 6 of the 13 contacts of KS-affected patients (46.2% of them) were infected with KSHV. In contrast to other researchers, we did not find in the populations that we studied in Cuba that KSHV seropositivity was associated with male homosexual or bisexual activity. We found high KSHV seropositivity rates among women reporting sexual contact with bisexual men and among men who had acquired an HIV infection in Africa. There were low rates of KSHV infection among the blood donors (1.2%) and the renal transplant recipients (0.0%). The low rates of KSHV infection that we found among the non-HIV-infected populations in Cuba are similar to patterns found in populations in Europe and in the United States. CONCLUSIONS: Together with similar results from Brazil, Jamaica, and the United States of America, our results ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic
institution Open Polar
collection Directory of Open Access Journals: DOAJ Articles
op_collection_id ftdoajarticles
language English
Spanish
Portuguese
topic Herpesvirus 8
sarcoma
HIV
acquired immunodeficiency syndrome
risk factors
seroepidemiologic studies
Cuba
Medicine
R
Arctic medicine. Tropical medicine
RC955-962
Public aspects of medicine
RA1-1270
spellingShingle Herpesvirus 8
sarcoma
HIV
acquired immunodeficiency syndrome
risk factors
seroepidemiologic studies
Cuba
Medicine
R
Arctic medicine. Tropical medicine
RC955-962
Public aspects of medicine
RA1-1270
Vivian Kourí
Sybil M. Eng
María E. Rodríguez
Sonia Resik
Odalys Orraca
Patrick S. Moore
Yuan Chang
Seroprevalence of Kaposi's sarcoma-associated herpesvirus in various populations in Cuba Seroprevalencia del herpesvirus asociado con el sarcoma de Kaposi en diversas poblaciones en Cuba
topic_facet Herpesvirus 8
sarcoma
HIV
acquired immunodeficiency syndrome
risk factors
seroepidemiologic studies
Cuba
Medicine
R
Arctic medicine. Tropical medicine
RC955-962
Public aspects of medicine
RA1-1270
description OBJECTIVE: Little is known about the prevalence and distribution of Kaposi's sarcoma-associated herpesvirus (KSHV) infection in the Caribbean. The aim of this study was to determine rates of KSHV seropositivity in various populations in Cuba. METHODS: During the years 1998 to 2002 we screened serum samples from 410 subjects in Cuba. Serologic screening for KSHV antibodies was a two-step process using (1) indirect immunofluorescence assay (IFA) specifically reactive to the KSHV latency-associated nuclear antigen (LANA) encoded by open reading frame 73 (ORF73), and (2) confirmatory immunoblot using recombinant KSHV ORF65.2, a lytically expressed, 20-kilodalton protein as the target antigen. Five different populations were studied: (1) 45 AIDS patients with Kaposi's sarcoma (AIDS-KS), (2) 154 HIV-1-infected patients without clinical evidence of KS, (3) 171 HIV-negative blood donors, (4) 27 consecutive kidney transplant recipients, who were HIV-negative, and (5) 13 contacts (sexual contacts or relatives) of the AIDS-KS-affected patients. RESULTS: Among the 45 AIDS-KS subjects, 35 of them (77.8%) were KSHV-seropositive. Thirty-two of the 154 HIV-positive patients without KS (20.8% of them) were KSHV-seropositive, and 6 of the 13 contacts of KS-affected patients (46.2% of them) were infected with KSHV. In contrast to other researchers, we did not find in the populations that we studied in Cuba that KSHV seropositivity was associated with male homosexual or bisexual activity. We found high KSHV seropositivity rates among women reporting sexual contact with bisexual men and among men who had acquired an HIV infection in Africa. There were low rates of KSHV infection among the blood donors (1.2%) and the renal transplant recipients (0.0%). The low rates of KSHV infection that we found among the non-HIV-infected populations in Cuba are similar to patterns found in populations in Europe and in the United States. CONCLUSIONS: Together with similar results from Brazil, Jamaica, and the United States of America, our results ...
format Article in Journal/Newspaper
author Vivian Kourí
Sybil M. Eng
María E. Rodríguez
Sonia Resik
Odalys Orraca
Patrick S. Moore
Yuan Chang
author_facet Vivian Kourí
Sybil M. Eng
María E. Rodríguez
Sonia Resik
Odalys Orraca
Patrick S. Moore
Yuan Chang
author_sort Vivian Kourí
title Seroprevalence of Kaposi's sarcoma-associated herpesvirus in various populations in Cuba Seroprevalencia del herpesvirus asociado con el sarcoma de Kaposi en diversas poblaciones en Cuba
title_short Seroprevalence of Kaposi's sarcoma-associated herpesvirus in various populations in Cuba Seroprevalencia del herpesvirus asociado con el sarcoma de Kaposi en diversas poblaciones en Cuba
title_full Seroprevalence of Kaposi's sarcoma-associated herpesvirus in various populations in Cuba Seroprevalencia del herpesvirus asociado con el sarcoma de Kaposi en diversas poblaciones en Cuba
title_fullStr Seroprevalence of Kaposi's sarcoma-associated herpesvirus in various populations in Cuba Seroprevalencia del herpesvirus asociado con el sarcoma de Kaposi en diversas poblaciones en Cuba
title_full_unstemmed Seroprevalence of Kaposi's sarcoma-associated herpesvirus in various populations in Cuba Seroprevalencia del herpesvirus asociado con el sarcoma de Kaposi en diversas poblaciones en Cuba
title_sort seroprevalence of kaposi's sarcoma-associated herpesvirus in various populations in cuba seroprevalencia del herpesvirus asociado con el sarcoma de kaposi en diversas poblaciones en cuba
publisher Pan American Health Organization
publishDate 2004
url https://doaj.org/article/aaaf011e296a4419aff0b1f13259d501
geographic Arctic
geographic_facet Arctic
genre Arctic
genre_facet Arctic
op_source Revista Panamericana de Salud Pública, Vol 15, Iss 5, Pp 320-325 (2004)
op_relation http://www.scielosp.org/scielo.php?script=sci_arttext&pid=S1020-49892004000500006
https://doaj.org/toc/1020-4989
https://doaj.org/toc/1680-5348
1020-4989
1680-5348
https://doaj.org/article/aaaf011e296a4419aff0b1f13259d501
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