Electric nets and sticky materials for analysing oviposition behaviour of gravid malaria vectors
Abstract Background Little is known about how malaria mosquitoes locate oviposition sites in nature. Such knowledge is important to help devise monitoring and control measures that could be used to target gravid females. This study set out to develop a suite of tools that can be used to study the at...
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ftdoajarticles:oai:doaj.org/article:aa8355baf499411db2a5e3e5e203c95a 2023-05-15T15:18:03+02:00 Electric nets and sticky materials for analysing oviposition behaviour of gravid malaria vectors Dugassa Sisay Lindh Jenny M Torr Steve J Oyieke Florence Lindsay Steven W Fillinger Ulrike 2012-11-01T00:00:00Z https://doi.org/10.1186/1475-2875-11-374 https://doaj.org/article/aa8355baf499411db2a5e3e5e203c95a EN eng BMC http://www.malariajournal.com/content/11/1/374 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-374 1475-2875 https://doaj.org/article/aa8355baf499411db2a5e3e5e203c95a Malaria Journal, Vol 11, Iss 1, p 374 (2012) Malaria Anopheles gambiae Oviposition Electric nets Sticky film Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2012 ftdoajarticles https://doi.org/10.1186/1475-2875-11-374 2022-12-31T13:43:54Z Abstract Background Little is known about how malaria mosquitoes locate oviposition sites in nature. Such knowledge is important to help devise monitoring and control measures that could be used to target gravid females. This study set out to develop a suite of tools that can be used to study the attraction of gravid Anopheles gambiae s.s. towards visual or olfactory cues associated with aquatic habitats. Methods Firstly, the study developed and assessed methods for using electrocuting nets to analyse the orientation of gravid females towards an aquatic habitat. Electric nets (1m high × 0.5m wide) were powered by a 12V battery via a spark box. High and low energy settings were compared for mosquito electrocution and a collection device developed to retain electrocuted mosquitoes when falling to the ground. Secondly, a range of sticky materials and a detergent were tested to quantify if and where gravid females land to lay their eggs, by treating the edge of the ponds and the water surface. A randomized complete block design was used for all experiments with 200 mosquitoes released each day. Experiments were conducted in screened semi-field systems using insectary-reared An. gambiae s.s. Data were analysed by generalized estimating equations. Results An electric net operated at the highest spark box energy of a 400 volt direct current made the net spark, creating a crackling sound, a burst of light and a burning smell. This setting caught 64% less mosquitoes than a net powered by reduced voltage output that could neither be heard nor seen (odds ratio (OR) 0.46; 95% confidence interval (CI) 0.40-0.53, p < 0.001). Three sticky boards (transparent film, glue coated black fly-screen and yellow film) were evaluated as catching devices under electric nets and the transparent and shiny black surfaces were found highly attractive (OR 41.6, 95% CI 19.8 – 87.3, p < 0.001 and OR 28.8, 95% CI 14.5 – 56.8, p < 0.001, respectively) for gravid mosquitoes to land on compared to a yellow sticky film board and therefore ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 11 1 374 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Malaria Anopheles gambiae Oviposition Electric nets Sticky film Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Malaria Anopheles gambiae Oviposition Electric nets Sticky film Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Dugassa Sisay Lindh Jenny M Torr Steve J Oyieke Florence Lindsay Steven W Fillinger Ulrike Electric nets and sticky materials for analysing oviposition behaviour of gravid malaria vectors |
topic_facet |
Malaria Anopheles gambiae Oviposition Electric nets Sticky film Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Little is known about how malaria mosquitoes locate oviposition sites in nature. Such knowledge is important to help devise monitoring and control measures that could be used to target gravid females. This study set out to develop a suite of tools that can be used to study the attraction of gravid Anopheles gambiae s.s. towards visual or olfactory cues associated with aquatic habitats. Methods Firstly, the study developed and assessed methods for using electrocuting nets to analyse the orientation of gravid females towards an aquatic habitat. Electric nets (1m high × 0.5m wide) were powered by a 12V battery via a spark box. High and low energy settings were compared for mosquito electrocution and a collection device developed to retain electrocuted mosquitoes when falling to the ground. Secondly, a range of sticky materials and a detergent were tested to quantify if and where gravid females land to lay their eggs, by treating the edge of the ponds and the water surface. A randomized complete block design was used for all experiments with 200 mosquitoes released each day. Experiments were conducted in screened semi-field systems using insectary-reared An. gambiae s.s. Data were analysed by generalized estimating equations. Results An electric net operated at the highest spark box energy of a 400 volt direct current made the net spark, creating a crackling sound, a burst of light and a burning smell. This setting caught 64% less mosquitoes than a net powered by reduced voltage output that could neither be heard nor seen (odds ratio (OR) 0.46; 95% confidence interval (CI) 0.40-0.53, p < 0.001). Three sticky boards (transparent film, glue coated black fly-screen and yellow film) were evaluated as catching devices under electric nets and the transparent and shiny black surfaces were found highly attractive (OR 41.6, 95% CI 19.8 – 87.3, p < 0.001 and OR 28.8, 95% CI 14.5 – 56.8, p < 0.001, respectively) for gravid mosquitoes to land on compared to a yellow sticky film board and therefore ... |
format |
Article in Journal/Newspaper |
author |
Dugassa Sisay Lindh Jenny M Torr Steve J Oyieke Florence Lindsay Steven W Fillinger Ulrike |
author_facet |
Dugassa Sisay Lindh Jenny M Torr Steve J Oyieke Florence Lindsay Steven W Fillinger Ulrike |
author_sort |
Dugassa Sisay |
title |
Electric nets and sticky materials for analysing oviposition behaviour of gravid malaria vectors |
title_short |
Electric nets and sticky materials for analysing oviposition behaviour of gravid malaria vectors |
title_full |
Electric nets and sticky materials for analysing oviposition behaviour of gravid malaria vectors |
title_fullStr |
Electric nets and sticky materials for analysing oviposition behaviour of gravid malaria vectors |
title_full_unstemmed |
Electric nets and sticky materials for analysing oviposition behaviour of gravid malaria vectors |
title_sort |
electric nets and sticky materials for analysing oviposition behaviour of gravid malaria vectors |
publisher |
BMC |
publishDate |
2012 |
url |
https://doi.org/10.1186/1475-2875-11-374 https://doaj.org/article/aa8355baf499411db2a5e3e5e203c95a |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 11, Iss 1, p 374 (2012) |
op_relation |
http://www.malariajournal.com/content/11/1/374 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-11-374 1475-2875 https://doaj.org/article/aa8355baf499411db2a5e3e5e203c95a |
op_doi |
https://doi.org/10.1186/1475-2875-11-374 |
container_title |
Malaria Journal |
container_volume |
11 |
container_issue |
1 |
container_start_page |
374 |
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1766348280623529984 |