Effects of Clostridium autoethanogenum protein on growth performance, intestine and liver health of turbot Scophthalmus maximus during grow-out phase
Clostridium autoethanogenum protein (CAP) is an emerging protein source with several key advantages and it has investigated on juvenile turbot (9.13 g). However, it was indicated that fish growth stage is a decisive factor for CAP performance and the influence of CAP on turbot intestine health has n...
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ftdoajarticles:oai:doaj.org/article:aa7cc82f1c814b788085bd8cadeccd2e 2024-09-15T18:34:00+00:00 Effects of Clostridium autoethanogenum protein on growth performance, intestine and liver health of turbot Scophthalmus maximus during grow-out phase Nan Bai Qing Li Shihui Pan Zezheng Qi Wanzhen Deng Min Gu 2024-06-01T00:00:00Z https://doi.org/10.1016/j.aqrep.2024.102076 https://doaj.org/article/aa7cc82f1c814b788085bd8cadeccd2e EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S2352513424001649 https://doaj.org/toc/2352-5134 2352-5134 doi:10.1016/j.aqrep.2024.102076 https://doaj.org/article/aa7cc82f1c814b788085bd8cadeccd2e Aquaculture Reports, Vol 36, Iss , Pp 102076- (2024) Clostridium autoethanogenum Scophthalmus maximus Intestine Liver Lipid metabolism Aquaculture. Fisheries. Angling SH1-691 article 2024 ftdoajarticles https://doi.org/10.1016/j.aqrep.2024.102076 2024-08-26T15:21:18Z Clostridium autoethanogenum protein (CAP) is an emerging protein source with several key advantages and it has investigated on juvenile turbot (9.13 g). However, it was indicated that fish growth stage is a decisive factor for CAP performance and the influence of CAP on turbot intestine health has not been reported. Thus, it is worth investigating the effects of CAP on turbot extensively. In the present study, a 67-day feeding experiment was conducted to evaluate the effects of CAP on turbot in the grow-out phase from growth performance, intestine and liver health. Six hundred and thirty turbot with an initial body weight of about 150 g were randomly divided into 6 groups, which were fed with fishmeal-based control diet or diet with CAP to replace 15%, 30%, 45%, 60% and 75% of fishmeal protein in control diet, respectively. From growth and feed utilization, the substitution level of CAP should not exceed 30%, which was less than previous reported on juvenile turbot. The palatability of the feed was linearly reduced. Although CAP did not induce enteritis, it decreased the intestine villi height in middle intestine and villi density in distal intestine. Except for decreasing the size of hepatocytes by CAP at 45% or higher substitution levels, no other abnormality observed in liver histology. However, dietary CAP reduced the triglyceride and cholesterol levels in the plasma at 30% or higher levels and linearly down-regulated genes related to lipogenesis in liver. The genes related lipid transportation and oxidation were up-regulated by CAP at the substitution levels of 15–60%. The present work confirmed the safety of CAP in fish feed and suggested more work should be conducted before it widely used in aquafeed. Article in Journal/Newspaper Scophthalmus maximus Turbot Directory of Open Access Journals: DOAJ Articles Aquaculture Reports 36 102076 |
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Directory of Open Access Journals: DOAJ Articles |
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language |
English |
topic |
Clostridium autoethanogenum Scophthalmus maximus Intestine Liver Lipid metabolism Aquaculture. Fisheries. Angling SH1-691 |
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Clostridium autoethanogenum Scophthalmus maximus Intestine Liver Lipid metabolism Aquaculture. Fisheries. Angling SH1-691 Nan Bai Qing Li Shihui Pan Zezheng Qi Wanzhen Deng Min Gu Effects of Clostridium autoethanogenum protein on growth performance, intestine and liver health of turbot Scophthalmus maximus during grow-out phase |
topic_facet |
Clostridium autoethanogenum Scophthalmus maximus Intestine Liver Lipid metabolism Aquaculture. Fisheries. Angling SH1-691 |
description |
Clostridium autoethanogenum protein (CAP) is an emerging protein source with several key advantages and it has investigated on juvenile turbot (9.13 g). However, it was indicated that fish growth stage is a decisive factor for CAP performance and the influence of CAP on turbot intestine health has not been reported. Thus, it is worth investigating the effects of CAP on turbot extensively. In the present study, a 67-day feeding experiment was conducted to evaluate the effects of CAP on turbot in the grow-out phase from growth performance, intestine and liver health. Six hundred and thirty turbot with an initial body weight of about 150 g were randomly divided into 6 groups, which were fed with fishmeal-based control diet or diet with CAP to replace 15%, 30%, 45%, 60% and 75% of fishmeal protein in control diet, respectively. From growth and feed utilization, the substitution level of CAP should not exceed 30%, which was less than previous reported on juvenile turbot. The palatability of the feed was linearly reduced. Although CAP did not induce enteritis, it decreased the intestine villi height in middle intestine and villi density in distal intestine. Except for decreasing the size of hepatocytes by CAP at 45% or higher substitution levels, no other abnormality observed in liver histology. However, dietary CAP reduced the triglyceride and cholesterol levels in the plasma at 30% or higher levels and linearly down-regulated genes related to lipogenesis in liver. The genes related lipid transportation and oxidation were up-regulated by CAP at the substitution levels of 15–60%. The present work confirmed the safety of CAP in fish feed and suggested more work should be conducted before it widely used in aquafeed. |
format |
Article in Journal/Newspaper |
author |
Nan Bai Qing Li Shihui Pan Zezheng Qi Wanzhen Deng Min Gu |
author_facet |
Nan Bai Qing Li Shihui Pan Zezheng Qi Wanzhen Deng Min Gu |
author_sort |
Nan Bai |
title |
Effects of Clostridium autoethanogenum protein on growth performance, intestine and liver health of turbot Scophthalmus maximus during grow-out phase |
title_short |
Effects of Clostridium autoethanogenum protein on growth performance, intestine and liver health of turbot Scophthalmus maximus during grow-out phase |
title_full |
Effects of Clostridium autoethanogenum protein on growth performance, intestine and liver health of turbot Scophthalmus maximus during grow-out phase |
title_fullStr |
Effects of Clostridium autoethanogenum protein on growth performance, intestine and liver health of turbot Scophthalmus maximus during grow-out phase |
title_full_unstemmed |
Effects of Clostridium autoethanogenum protein on growth performance, intestine and liver health of turbot Scophthalmus maximus during grow-out phase |
title_sort |
effects of clostridium autoethanogenum protein on growth performance, intestine and liver health of turbot scophthalmus maximus during grow-out phase |
publisher |
Elsevier |
publishDate |
2024 |
url |
https://doi.org/10.1016/j.aqrep.2024.102076 https://doaj.org/article/aa7cc82f1c814b788085bd8cadeccd2e |
genre |
Scophthalmus maximus Turbot |
genre_facet |
Scophthalmus maximus Turbot |
op_source |
Aquaculture Reports, Vol 36, Iss , Pp 102076- (2024) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2352513424001649 https://doaj.org/toc/2352-5134 2352-5134 doi:10.1016/j.aqrep.2024.102076 https://doaj.org/article/aa7cc82f1c814b788085bd8cadeccd2e |
op_doi |
https://doi.org/10.1016/j.aqrep.2024.102076 |
container_title |
Aquaculture Reports |
container_volume |
36 |
container_start_page |
102076 |
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1810475736178360320 |