Spatio-temporal characterization of Trypanosoma cruzi infection and discrete typing units infecting hosts and vectors from non-domestic foci of Chile.
BACKGROUND:Trypanosoma cruzi is a protozoan parasite that is transmitted by triatomine vectors to mammals. It is classified in six discrete typing units (DTUs). In Chile, domestic vectorial transmission has been interrupted; however, the parasite is maintained in non-domestic foci. The aim of this s...
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Online Access: | https://doi.org/10.1371/journal.pntd.0007170 https://doaj.org/article/a9d9abcd232b4a7fbcecbe3bc781070a |
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ftdoajarticles:oai:doaj.org/article:a9d9abcd232b4a7fbcecbe3bc781070a 2023-05-15T15:16:22+02:00 Spatio-temporal characterization of Trypanosoma cruzi infection and discrete typing units infecting hosts and vectors from non-domestic foci of Chile. Camila Ihle-Soto Eduardo Costoya Juana P Correa Antonella Bacigalupo Berenice Cornejo-Villar Viviana Estadella Aldo Solari Sylvia Ortiz Héctor J Hernández Carezza Botto-Mahan David E Gorla Pedro E Cattan 2019-02-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007170 https://doaj.org/article/a9d9abcd232b4a7fbcecbe3bc781070a EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC6395009?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007170 https://doaj.org/article/a9d9abcd232b4a7fbcecbe3bc781070a PLoS Neglected Tropical Diseases, Vol 13, Iss 2, p e0007170 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007170 2022-12-31T13:23:20Z BACKGROUND:Trypanosoma cruzi is a protozoan parasite that is transmitted by triatomine vectors to mammals. It is classified in six discrete typing units (DTUs). In Chile, domestic vectorial transmission has been interrupted; however, the parasite is maintained in non-domestic foci. The aim of this study was to describe T. cruzi infection and DTU composition in mammals and triatomines from several non-domestic populations of North-Central Chile and to evaluate their spatio-temporal variations. METHODOLOGY/PRINCIPAL FINDINGS:A total of 710 small mammals and 1140 triatomines captured in six localities during two study periods (summer/winter) of the same year were analyzed by conventional PCR to detect kDNA of T. cruzi. Positive samples were DNA blotted and hybridized with specific probes for detection of DTUs TcI, TcII, TcV, and TcVI. Infection status was modeled, and cluster analysis was performed in each locality. We detected 30.1% of overall infection in small mammals and 34.1% in triatomines, with higher rates in synanthropic mammals and in M. spinolai. We identified infecting DTUs in 45 mammals and 110 triatomines, present more commonly as single infections; the most frequent DTU detected was TcI. Differences in infection rates among species, localities and study periods were detected in small mammals, and between triatomine species; temporally, infection presented opposite patterns between mammals and triatomines. Infection clustering was frequent in vectors, and one locality exhibited half of the 21 clusters found. CONCLUSIONS/SIGNIFICANCE:We determined T. cruzi infection in natural host and vector populations simultaneously in a spatially widespread manner during two study periods. All captured species presented T. cruzi infection, showing spatial and temporal variations. Trypanosoma cruzi distribution can be clustered in space and time. These clusters may represent different spatial and temporal risks of transmission. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 13 2 e0007170 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Camila Ihle-Soto Eduardo Costoya Juana P Correa Antonella Bacigalupo Berenice Cornejo-Villar Viviana Estadella Aldo Solari Sylvia Ortiz Héctor J Hernández Carezza Botto-Mahan David E Gorla Pedro E Cattan Spatio-temporal characterization of Trypanosoma cruzi infection and discrete typing units infecting hosts and vectors from non-domestic foci of Chile. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:Trypanosoma cruzi is a protozoan parasite that is transmitted by triatomine vectors to mammals. It is classified in six discrete typing units (DTUs). In Chile, domestic vectorial transmission has been interrupted; however, the parasite is maintained in non-domestic foci. The aim of this study was to describe T. cruzi infection and DTU composition in mammals and triatomines from several non-domestic populations of North-Central Chile and to evaluate their spatio-temporal variations. METHODOLOGY/PRINCIPAL FINDINGS:A total of 710 small mammals and 1140 triatomines captured in six localities during two study periods (summer/winter) of the same year were analyzed by conventional PCR to detect kDNA of T. cruzi. Positive samples were DNA blotted and hybridized with specific probes for detection of DTUs TcI, TcII, TcV, and TcVI. Infection status was modeled, and cluster analysis was performed in each locality. We detected 30.1% of overall infection in small mammals and 34.1% in triatomines, with higher rates in synanthropic mammals and in M. spinolai. We identified infecting DTUs in 45 mammals and 110 triatomines, present more commonly as single infections; the most frequent DTU detected was TcI. Differences in infection rates among species, localities and study periods were detected in small mammals, and between triatomine species; temporally, infection presented opposite patterns between mammals and triatomines. Infection clustering was frequent in vectors, and one locality exhibited half of the 21 clusters found. CONCLUSIONS/SIGNIFICANCE:We determined T. cruzi infection in natural host and vector populations simultaneously in a spatially widespread manner during two study periods. All captured species presented T. cruzi infection, showing spatial and temporal variations. Trypanosoma cruzi distribution can be clustered in space and time. These clusters may represent different spatial and temporal risks of transmission. |
format |
Article in Journal/Newspaper |
author |
Camila Ihle-Soto Eduardo Costoya Juana P Correa Antonella Bacigalupo Berenice Cornejo-Villar Viviana Estadella Aldo Solari Sylvia Ortiz Héctor J Hernández Carezza Botto-Mahan David E Gorla Pedro E Cattan |
author_facet |
Camila Ihle-Soto Eduardo Costoya Juana P Correa Antonella Bacigalupo Berenice Cornejo-Villar Viviana Estadella Aldo Solari Sylvia Ortiz Héctor J Hernández Carezza Botto-Mahan David E Gorla Pedro E Cattan |
author_sort |
Camila Ihle-Soto |
title |
Spatio-temporal characterization of Trypanosoma cruzi infection and discrete typing units infecting hosts and vectors from non-domestic foci of Chile. |
title_short |
Spatio-temporal characterization of Trypanosoma cruzi infection and discrete typing units infecting hosts and vectors from non-domestic foci of Chile. |
title_full |
Spatio-temporal characterization of Trypanosoma cruzi infection and discrete typing units infecting hosts and vectors from non-domestic foci of Chile. |
title_fullStr |
Spatio-temporal characterization of Trypanosoma cruzi infection and discrete typing units infecting hosts and vectors from non-domestic foci of Chile. |
title_full_unstemmed |
Spatio-temporal characterization of Trypanosoma cruzi infection and discrete typing units infecting hosts and vectors from non-domestic foci of Chile. |
title_sort |
spatio-temporal characterization of trypanosoma cruzi infection and discrete typing units infecting hosts and vectors from non-domestic foci of chile. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007170 https://doaj.org/article/a9d9abcd232b4a7fbcecbe3bc781070a |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 2, p e0007170 (2019) |
op_relation |
http://europepmc.org/articles/PMC6395009?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007170 https://doaj.org/article/a9d9abcd232b4a7fbcecbe3bc781070a |
op_doi |
https://doi.org/10.1371/journal.pntd.0007170 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
13 |
container_issue |
2 |
container_start_page |
e0007170 |
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1766346656923516928 |