A glance of the blood stage transcriptome of a Southeast Asian Plasmodium ovale isolate.
Plasmodium ovale accounts for a disproportionate number of travel-related malaria cases. This parasite is understudied since there is a reliance on clinical samples. We collected a P. ovale curtisi parasite isolate from a clinical case in western Thailand and performed RNA-seq analysis on the blood...
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Online Access: | https://doi.org/10.1371/journal.pntd.0007850 https://doaj.org/article/a9d8e523bccd4a64a5eb3707ab6c2efc |
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ftdoajarticles:oai:doaj.org/article:a9d8e523bccd4a64a5eb3707ab6c2efc 2023-05-15T15:04:14+02:00 A glance of the blood stage transcriptome of a Southeast Asian Plasmodium ovale isolate. Awtum M Brashear Wanlapa Roobsoong Faiza A Siddiqui Wang Nguitragool Jetsumon Sattabongkot Margarita M López-Uribe Jun Miao Liwang Cui 2019-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0007850 https://doaj.org/article/a9d8e523bccd4a64a5eb3707ab6c2efc EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0007850 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007850 https://doaj.org/article/a9d8e523bccd4a64a5eb3707ab6c2efc PLoS Neglected Tropical Diseases, Vol 13, Iss 11, p e0007850 (2019) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2019 ftdoajarticles https://doi.org/10.1371/journal.pntd.0007850 2022-12-31T07:50:09Z Plasmodium ovale accounts for a disproportionate number of travel-related malaria cases. This parasite is understudied since there is a reliance on clinical samples. We collected a P. ovale curtisi parasite isolate from a clinical case in western Thailand and performed RNA-seq analysis on the blood stage transcriptomes. Using both de novo assembly and alignment-based methods, we detected the transcripts for 6628 out of 7280 annotated genes. For those lacking evidence of expression, the vast majority belonged to the PIR and STP1 gene families. We identified new splicing patterns for over 2500 genes, and mapped at least one untranslated region for over half of all annotated genes. Our analysis also detected a notable presence of anti-sense transcripts for over 10% of P. ovale curtisi genes. This transcriptomic analysis provides new insights into the blood-stage biology of this neglected parasite. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 13 11 e0007850 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Awtum M Brashear Wanlapa Roobsoong Faiza A Siddiqui Wang Nguitragool Jetsumon Sattabongkot Margarita M López-Uribe Jun Miao Liwang Cui A glance of the blood stage transcriptome of a Southeast Asian Plasmodium ovale isolate. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Plasmodium ovale accounts for a disproportionate number of travel-related malaria cases. This parasite is understudied since there is a reliance on clinical samples. We collected a P. ovale curtisi parasite isolate from a clinical case in western Thailand and performed RNA-seq analysis on the blood stage transcriptomes. Using both de novo assembly and alignment-based methods, we detected the transcripts for 6628 out of 7280 annotated genes. For those lacking evidence of expression, the vast majority belonged to the PIR and STP1 gene families. We identified new splicing patterns for over 2500 genes, and mapped at least one untranslated region for over half of all annotated genes. Our analysis also detected a notable presence of anti-sense transcripts for over 10% of P. ovale curtisi genes. This transcriptomic analysis provides new insights into the blood-stage biology of this neglected parasite. |
format |
Article in Journal/Newspaper |
author |
Awtum M Brashear Wanlapa Roobsoong Faiza A Siddiqui Wang Nguitragool Jetsumon Sattabongkot Margarita M López-Uribe Jun Miao Liwang Cui |
author_facet |
Awtum M Brashear Wanlapa Roobsoong Faiza A Siddiqui Wang Nguitragool Jetsumon Sattabongkot Margarita M López-Uribe Jun Miao Liwang Cui |
author_sort |
Awtum M Brashear |
title |
A glance of the blood stage transcriptome of a Southeast Asian Plasmodium ovale isolate. |
title_short |
A glance of the blood stage transcriptome of a Southeast Asian Plasmodium ovale isolate. |
title_full |
A glance of the blood stage transcriptome of a Southeast Asian Plasmodium ovale isolate. |
title_fullStr |
A glance of the blood stage transcriptome of a Southeast Asian Plasmodium ovale isolate. |
title_full_unstemmed |
A glance of the blood stage transcriptome of a Southeast Asian Plasmodium ovale isolate. |
title_sort |
glance of the blood stage transcriptome of a southeast asian plasmodium ovale isolate. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2019 |
url |
https://doi.org/10.1371/journal.pntd.0007850 https://doaj.org/article/a9d8e523bccd4a64a5eb3707ab6c2efc |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 13, Iss 11, p e0007850 (2019) |
op_relation |
https://doi.org/10.1371/journal.pntd.0007850 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0007850 https://doaj.org/article/a9d8e523bccd4a64a5eb3707ab6c2efc |
op_doi |
https://doi.org/10.1371/journal.pntd.0007850 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
13 |
container_issue |
11 |
container_start_page |
e0007850 |
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1766336034418720768 |