Screening, isolation and optimization of anti–white spot syndrome virus drug derived from marine plants
Objective: To screen, isolate and optimize anti-white spot syndrome virus (WSSV) drug derived from various marine floral ecosystems and to evaluate the efficacy of the same in host–pathogen interaction model. Methods: Thirty species of marine plants were subjected to Soxhlet extraction using water,...
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Wolters Kluwer Medknow Publications
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ftdoajarticles:oai:doaj.org/article:a9236174bbe842a8a23606b12d96e0f4 2023-05-15T15:08:04+02:00 Screening, isolation and optimization of anti–white spot syndrome virus drug derived from marine plants Somnath Chakraborty Upasana Ghosh Thangavel Balasubramanian Punyabrata Das 2014-05-01T00:00:00Z https://doi.org/10.12980/APJTB.4.2014C1037 https://doaj.org/article/a9236174bbe842a8a23606b12d96e0f4 EN eng Wolters Kluwer Medknow Publications http://www.sciencedirect.com/science/article/pii/S2221169115302537 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.2014C1037 https://doaj.org/article/a9236174bbe842a8a23606b12d96e0f4 Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss S1, Pp S107-S117 (2014) Shrimps Litopenaeus vannamei Anti-WSSV Marine plants MP07X White spot syndrome virus Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 article 2014 ftdoajarticles https://doi.org/10.12980/APJTB.4.2014C1037 2022-12-31T12:37:29Z Objective: To screen, isolate and optimize anti-white spot syndrome virus (WSSV) drug derived from various marine floral ecosystems and to evaluate the efficacy of the same in host–pathogen interaction model. Methods: Thirty species of marine plants were subjected to Soxhlet extraction using water, ethanol, methanol and hexane as solvents. The 120 plant isolates thus obtained were screened for their in vivo anti-WSSV property in Litopenaeus vannamei. By means of chemical processes, the purified anti-WSSV plant isolate, MP07X was derived. The drug was optimized at various concentrations. Viral and immune genes were analysed using reverse transcriptase PCR to confirm the potency of the drug. Results: Nine plant isolates exhibited significant survivability in host. The drug MP07X thus formulated showing 85% survivability in host. The surviving shrimps were nested PCR negative at the end of the 15 d experimentation. The lowest concentration of MP07X required intramuscularly for virucidal property was 10 mg/mL. The oral dosage of 1 000 mg/kg body weight/day survived at the rate of 85%. Neither VP28 nor ie 1 was expressed in the test samples at 42nd hour and 84th hour post viral infection. Conclusions: The drug MP07X derived from Rhizophora mucronata is a potent anti-WSSV drug. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Asian Pacific Journal of Tropical Biomedicine 4 S107 S117 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Shrimps Litopenaeus vannamei Anti-WSSV Marine plants MP07X White spot syndrome virus Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
spellingShingle |
Shrimps Litopenaeus vannamei Anti-WSSV Marine plants MP07X White spot syndrome virus Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 Somnath Chakraborty Upasana Ghosh Thangavel Balasubramanian Punyabrata Das Screening, isolation and optimization of anti–white spot syndrome virus drug derived from marine plants |
topic_facet |
Shrimps Litopenaeus vannamei Anti-WSSV Marine plants MP07X White spot syndrome virus Arctic medicine. Tropical medicine RC955-962 Biology (General) QH301-705.5 |
description |
Objective: To screen, isolate and optimize anti-white spot syndrome virus (WSSV) drug derived from various marine floral ecosystems and to evaluate the efficacy of the same in host–pathogen interaction model. Methods: Thirty species of marine plants were subjected to Soxhlet extraction using water, ethanol, methanol and hexane as solvents. The 120 plant isolates thus obtained were screened for their in vivo anti-WSSV property in Litopenaeus vannamei. By means of chemical processes, the purified anti-WSSV plant isolate, MP07X was derived. The drug was optimized at various concentrations. Viral and immune genes were analysed using reverse transcriptase PCR to confirm the potency of the drug. Results: Nine plant isolates exhibited significant survivability in host. The drug MP07X thus formulated showing 85% survivability in host. The surviving shrimps were nested PCR negative at the end of the 15 d experimentation. The lowest concentration of MP07X required intramuscularly for virucidal property was 10 mg/mL. The oral dosage of 1 000 mg/kg body weight/day survived at the rate of 85%. Neither VP28 nor ie 1 was expressed in the test samples at 42nd hour and 84th hour post viral infection. Conclusions: The drug MP07X derived from Rhizophora mucronata is a potent anti-WSSV drug. |
format |
Article in Journal/Newspaper |
author |
Somnath Chakraborty Upasana Ghosh Thangavel Balasubramanian Punyabrata Das |
author_facet |
Somnath Chakraborty Upasana Ghosh Thangavel Balasubramanian Punyabrata Das |
author_sort |
Somnath Chakraborty |
title |
Screening, isolation and optimization of anti–white spot syndrome virus drug derived from marine plants |
title_short |
Screening, isolation and optimization of anti–white spot syndrome virus drug derived from marine plants |
title_full |
Screening, isolation and optimization of anti–white spot syndrome virus drug derived from marine plants |
title_fullStr |
Screening, isolation and optimization of anti–white spot syndrome virus drug derived from marine plants |
title_full_unstemmed |
Screening, isolation and optimization of anti–white spot syndrome virus drug derived from marine plants |
title_sort |
screening, isolation and optimization of anti–white spot syndrome virus drug derived from marine plants |
publisher |
Wolters Kluwer Medknow Publications |
publishDate |
2014 |
url |
https://doi.org/10.12980/APJTB.4.2014C1037 https://doaj.org/article/a9236174bbe842a8a23606b12d96e0f4 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Asian Pacific Journal of Tropical Biomedicine, Vol 4, Iss S1, Pp S107-S117 (2014) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2221169115302537 https://doaj.org/toc/2221-1691 2221-1691 doi:10.12980/APJTB.4.2014C1037 https://doaj.org/article/a9236174bbe842a8a23606b12d96e0f4 |
op_doi |
https://doi.org/10.12980/APJTB.4.2014C1037 |
container_title |
Asian Pacific Journal of Tropical Biomedicine |
container_volume |
4 |
container_start_page |
S107 |
op_container_end_page |
S117 |
_version_ |
1766339502624735232 |