Identification of Melanin in the Mantle of the Pacific Oyster Crassostrea gigas
The Pacific oyster, Crassostrea gigas, is widely distributed and is substantially important to shellfish cultivation. Oysters with melanin-colored shells and soft parts are increasingly popular and are of high economic value. However, the distribution and synthesis of melanin in C. gigas remains unc...
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Online Access: | https://doi.org/10.3389/fmars.2022.880337 https://doaj.org/article/a90ba6a94fd84da893063f56c1985c7d |
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ftdoajarticles:oai:doaj.org/article:a90ba6a94fd84da893063f56c1985c7d 2023-05-15T15:57:37+02:00 Identification of Melanin in the Mantle of the Pacific Oyster Crassostrea gigas Yijing Han Chaoyi Xie Nini Fan Hongce Song Xiaomei Wang Yanxin Zheng Meiwei Zhang Yaqiong Liu Baoyu Huang Lei Wei Xiaotong Wang 2022-03-01T00:00:00Z https://doi.org/10.3389/fmars.2022.880337 https://doaj.org/article/a90ba6a94fd84da893063f56c1985c7d EN eng Frontiers Media S.A. https://www.frontiersin.org/articles/10.3389/fmars.2022.880337/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2022.880337 https://doaj.org/article/a90ba6a94fd84da893063f56c1985c7d Frontiers in Marine Science, Vol 9 (2022) Crassostrea gigas mantle melanin melanocyte frozen section Science Q General. Including nature conservation geographical distribution QH1-199.5 article 2022 ftdoajarticles https://doi.org/10.3389/fmars.2022.880337 2022-12-31T13:25:38Z The Pacific oyster, Crassostrea gigas, is widely distributed and is substantially important to shellfish cultivation. Oysters with melanin-colored shells and soft parts are increasingly popular and are of high economic value. However, the distribution and synthesis of melanin in C. gigas remains unclear and there have been only few relevant studies on it. In this study, transmission electron microscopy (TEM) was used to observe melanin in the epidermis of dark mantle tissue. Melanocytes containing melanosomes were observed below the epidermis, suggesting the possibility of a gradual transfer of melanin from the mantle tissue to the epithelium. The frozen section technique and three melanin staining methods were used to confirm the presence of melanin. The results suggest that enzyme identification (DOPA) is a suitable method of melanin staining in the mantle tissues of C. gigas. This study preliminarily identified the existence of melanin in the mantle tissues of C. gigas and is a foundation for the study of melanin synthesis. Furthermore, it provides new insights into the mechanism of shell color formation. Article in Journal/Newspaper Crassostrea gigas Pacific oyster Directory of Open Access Journals: DOAJ Articles Pacific Frontiers in Marine Science 9 |
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Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Crassostrea gigas mantle melanin melanocyte frozen section Science Q General. Including nature conservation geographical distribution QH1-199.5 |
spellingShingle |
Crassostrea gigas mantle melanin melanocyte frozen section Science Q General. Including nature conservation geographical distribution QH1-199.5 Yijing Han Chaoyi Xie Nini Fan Hongce Song Xiaomei Wang Yanxin Zheng Meiwei Zhang Yaqiong Liu Baoyu Huang Lei Wei Xiaotong Wang Identification of Melanin in the Mantle of the Pacific Oyster Crassostrea gigas |
topic_facet |
Crassostrea gigas mantle melanin melanocyte frozen section Science Q General. Including nature conservation geographical distribution QH1-199.5 |
description |
The Pacific oyster, Crassostrea gigas, is widely distributed and is substantially important to shellfish cultivation. Oysters with melanin-colored shells and soft parts are increasingly popular and are of high economic value. However, the distribution and synthesis of melanin in C. gigas remains unclear and there have been only few relevant studies on it. In this study, transmission electron microscopy (TEM) was used to observe melanin in the epidermis of dark mantle tissue. Melanocytes containing melanosomes were observed below the epidermis, suggesting the possibility of a gradual transfer of melanin from the mantle tissue to the epithelium. The frozen section technique and three melanin staining methods were used to confirm the presence of melanin. The results suggest that enzyme identification (DOPA) is a suitable method of melanin staining in the mantle tissues of C. gigas. This study preliminarily identified the existence of melanin in the mantle tissues of C. gigas and is a foundation for the study of melanin synthesis. Furthermore, it provides new insights into the mechanism of shell color formation. |
format |
Article in Journal/Newspaper |
author |
Yijing Han Chaoyi Xie Nini Fan Hongce Song Xiaomei Wang Yanxin Zheng Meiwei Zhang Yaqiong Liu Baoyu Huang Lei Wei Xiaotong Wang |
author_facet |
Yijing Han Chaoyi Xie Nini Fan Hongce Song Xiaomei Wang Yanxin Zheng Meiwei Zhang Yaqiong Liu Baoyu Huang Lei Wei Xiaotong Wang |
author_sort |
Yijing Han |
title |
Identification of Melanin in the Mantle of the Pacific Oyster Crassostrea gigas |
title_short |
Identification of Melanin in the Mantle of the Pacific Oyster Crassostrea gigas |
title_full |
Identification of Melanin in the Mantle of the Pacific Oyster Crassostrea gigas |
title_fullStr |
Identification of Melanin in the Mantle of the Pacific Oyster Crassostrea gigas |
title_full_unstemmed |
Identification of Melanin in the Mantle of the Pacific Oyster Crassostrea gigas |
title_sort |
identification of melanin in the mantle of the pacific oyster crassostrea gigas |
publisher |
Frontiers Media S.A. |
publishDate |
2022 |
url |
https://doi.org/10.3389/fmars.2022.880337 https://doaj.org/article/a90ba6a94fd84da893063f56c1985c7d |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas Pacific oyster |
genre_facet |
Crassostrea gigas Pacific oyster |
op_source |
Frontiers in Marine Science, Vol 9 (2022) |
op_relation |
https://www.frontiersin.org/articles/10.3389/fmars.2022.880337/full https://doaj.org/toc/2296-7745 2296-7745 doi:10.3389/fmars.2022.880337 https://doaj.org/article/a90ba6a94fd84da893063f56c1985c7d |
op_doi |
https://doi.org/10.3389/fmars.2022.880337 |
container_title |
Frontiers in Marine Science |
container_volume |
9 |
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1766393288516960256 |