Di(2-ethylhexyl) adipate (DEHA) detection in Antarctic krill (Euphasia superba Dana)
In this reported study, a novel high-performance thin-layer chromatography (HPTLC) method was developed for the detection and quantification of the toxic substance di(2-ethylhexyl) adipate (DEHA) in Antarctic krill. This procedure was based on the extraction of DEHA by ultrasonic solvent extraction...
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Norwegian Polar Institute
2018
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ftdoajarticles:oai:doaj.org/article:a8ce9534c2b449638c8310f9a5c02d08 2023-05-15T13:34:55+02:00 Di(2-ethylhexyl) adipate (DEHA) detection in Antarctic krill (Euphasia superba Dana) Xiangning Han Daicheng Liu 2018-01-01T00:00:00Z https://doi.org/10.1080/17518369.2018.1457395 https://doaj.org/article/a8ce9534c2b449638c8310f9a5c02d08 EN eng Norwegian Polar Institute http://dx.doi.org/10.1080/17518369.2018.1457395 https://doaj.org/toc/1751-8369 1751-8369 doi:10.1080/17518369.2018.1457395 https://doaj.org/article/a8ce9534c2b449638c8310f9a5c02d08 Polar Research, Vol 37, Iss 1 (2018) toxicity high-performance thin-layer chromatography (HPTLC) ecotoxins plastic particles marine pollution krill oil Environmental sciences GE1-350 Oceanography GC1-1581 article 2018 ftdoajarticles https://doi.org/10.1080/17518369.2018.1457395 2022-12-31T04:30:58Z In this reported study, a novel high-performance thin-layer chromatography (HPTLC) method was developed for the detection and quantification of the toxic substance di(2-ethylhexyl) adipate (DEHA) in Antarctic krill. This procedure was based on the extraction of DEHA by ultrasonic solvent extraction with anhydrous ethanol, silica-gel column chromatographic separation, HPTLC detection and quantification using petroleum ether/ethyl acetate/ acetone/glacial acetic acid (29:1:0.5:2d*, v/v/v/v) as the developing solvent and bromine thymol blue solution as the chromogenic agent. The content of DEHA in freeze-dried Antarctic krill was found to be ca. 0.63 ± 0.05 mg/g. The structure of DEHA in the Antarctic krill was subsequently determined by gas chromatography–mass spectrometry (GC-MS) and infrared chromatography, which verified the presence of this compound in the krill. The HPTLC method exhibited excellent accuracy, with a recovery of 97.1–101.6% and good precision with a relative standard deviation of 2.47–4.90%. The DEHA in Antarctic krill oil was extracted by n-hexane and detected using the same method described above, which verified that DEHA was also present in krill oil at a concentration of ca. 2.16 ± 0.08 mg/g. The presence of DEHA in kill oil is very concerning because of its demonstrated harmful ecotoxicity, and since Antarctic krill is the key link in the food chain in the Antarctic coastal marine ecosystem. The adverse effects of DEHA on Antarctic krill and the source of DEHA will be explored in future research. Article in Journal/Newspaper Antarc* Antarctic Antarctic Krill Polar Research Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic Polar Research 37 1 1457395 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
toxicity high-performance thin-layer chromatography (HPTLC) ecotoxins plastic particles marine pollution krill oil Environmental sciences GE1-350 Oceanography GC1-1581 |
spellingShingle |
toxicity high-performance thin-layer chromatography (HPTLC) ecotoxins plastic particles marine pollution krill oil Environmental sciences GE1-350 Oceanography GC1-1581 Xiangning Han Daicheng Liu Di(2-ethylhexyl) adipate (DEHA) detection in Antarctic krill (Euphasia superba Dana) |
topic_facet |
toxicity high-performance thin-layer chromatography (HPTLC) ecotoxins plastic particles marine pollution krill oil Environmental sciences GE1-350 Oceanography GC1-1581 |
description |
In this reported study, a novel high-performance thin-layer chromatography (HPTLC) method was developed for the detection and quantification of the toxic substance di(2-ethylhexyl) adipate (DEHA) in Antarctic krill. This procedure was based on the extraction of DEHA by ultrasonic solvent extraction with anhydrous ethanol, silica-gel column chromatographic separation, HPTLC detection and quantification using petroleum ether/ethyl acetate/ acetone/glacial acetic acid (29:1:0.5:2d*, v/v/v/v) as the developing solvent and bromine thymol blue solution as the chromogenic agent. The content of DEHA in freeze-dried Antarctic krill was found to be ca. 0.63 ± 0.05 mg/g. The structure of DEHA in the Antarctic krill was subsequently determined by gas chromatography–mass spectrometry (GC-MS) and infrared chromatography, which verified the presence of this compound in the krill. The HPTLC method exhibited excellent accuracy, with a recovery of 97.1–101.6% and good precision with a relative standard deviation of 2.47–4.90%. The DEHA in Antarctic krill oil was extracted by n-hexane and detected using the same method described above, which verified that DEHA was also present in krill oil at a concentration of ca. 2.16 ± 0.08 mg/g. The presence of DEHA in kill oil is very concerning because of its demonstrated harmful ecotoxicity, and since Antarctic krill is the key link in the food chain in the Antarctic coastal marine ecosystem. The adverse effects of DEHA on Antarctic krill and the source of DEHA will be explored in future research. |
format |
Article in Journal/Newspaper |
author |
Xiangning Han Daicheng Liu |
author_facet |
Xiangning Han Daicheng Liu |
author_sort |
Xiangning Han |
title |
Di(2-ethylhexyl) adipate (DEHA) detection in Antarctic krill (Euphasia superba Dana) |
title_short |
Di(2-ethylhexyl) adipate (DEHA) detection in Antarctic krill (Euphasia superba Dana) |
title_full |
Di(2-ethylhexyl) adipate (DEHA) detection in Antarctic krill (Euphasia superba Dana) |
title_fullStr |
Di(2-ethylhexyl) adipate (DEHA) detection in Antarctic krill (Euphasia superba Dana) |
title_full_unstemmed |
Di(2-ethylhexyl) adipate (DEHA) detection in Antarctic krill (Euphasia superba Dana) |
title_sort |
di(2-ethylhexyl) adipate (deha) detection in antarctic krill (euphasia superba dana) |
publisher |
Norwegian Polar Institute |
publishDate |
2018 |
url |
https://doi.org/10.1080/17518369.2018.1457395 https://doaj.org/article/a8ce9534c2b449638c8310f9a5c02d08 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic Antarctic Krill Polar Research |
genre_facet |
Antarc* Antarctic Antarctic Krill Polar Research |
op_source |
Polar Research, Vol 37, Iss 1 (2018) |
op_relation |
http://dx.doi.org/10.1080/17518369.2018.1457395 https://doaj.org/toc/1751-8369 1751-8369 doi:10.1080/17518369.2018.1457395 https://doaj.org/article/a8ce9534c2b449638c8310f9a5c02d08 |
op_doi |
https://doi.org/10.1080/17518369.2018.1457395 |
container_title |
Polar Research |
container_volume |
37 |
container_issue |
1 |
container_start_page |
1457395 |
_version_ |
1766059053990019072 |