Schistosomiasis mansoni: evidence for a milder response in germfree mice
Germfree (GF and conventional (CV) mice were infected intraperitoneally with GF cercariae of Schistosoma mansoni and kept for six weeks. Twenty four hours before killing, they were injected with [³H]-thymidine. Schistosoma worms, harvested after perfusion of portal system, were counted as well as eg...
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Universidade de São Paulo (USP)
1987
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ftdoajarticles:oai:doaj.org/article:a8c01a8ea8a24aab8800d33547f5ebb2 2024-09-09T19:26:06+00:00 Schistosomiasis mansoni: evidence for a milder response in germfree mice Leda Q. Viera Tasso Moraes-santos 1987-02-01T00:00:00Z https://doaj.org/article/a8c01a8ea8a24aab8800d33547f5ebb2 EN eng Universidade de São Paulo (USP) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651987000100006&tlng=en https://doaj.org/toc/1678-9946 1678-9946 https://doaj.org/article/a8c01a8ea8a24aab8800d33547f5ebb2 Revista do Instituto de Medicina Tropical de São Paulo, Vol 29, Iss 1, Pp 37-42 (1987) Schistosomiasis mansoni Axenic cercariae Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 1987 ftdoajarticles 2024-08-05T17:49:31Z Germfree (GF and conventional (CV) mice were infected intraperitoneally with GF cercariae of Schistosoma mansoni and kept for six weeks. Twenty four hours before killing, they were injected with [³H]-thymidine. Schistosoma worms, harvested after perfusion of portal system, were counted as well as eggs from liver and intestines. Liver was also used for DNA, protein, and collagen determinations. [³H] -Thymidine incorporation and collagen determinations were used to establish the indices given by the difference between their contents in infected and control animals and expressed per thousand eggs in liver. The recovery of worms in GF mice was around twice as much as in CV ones, and the total number of eggs was higher in the liver of GF animals. No hypertrophy of liver cells was observed by the ratio protein/DNA, but [³H]-thymidine incorporation into DNA was higher than in controls in both GF and CV infected animals. The [³H]-thymidine and collagen indices were lower in GF animals which indicate a more discrete cellular replication and smaller collagen content in relation to the number of eggs present in livers of these mice. It was concluded that the disease seems to be less severe in GF animals. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Schistosomiasis mansoni Axenic cercariae Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Schistosomiasis mansoni Axenic cercariae Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Leda Q. Viera Tasso Moraes-santos Schistosomiasis mansoni: evidence for a milder response in germfree mice |
topic_facet |
Schistosomiasis mansoni Axenic cercariae Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Germfree (GF and conventional (CV) mice were infected intraperitoneally with GF cercariae of Schistosoma mansoni and kept for six weeks. Twenty four hours before killing, they were injected with [³H]-thymidine. Schistosoma worms, harvested after perfusion of portal system, were counted as well as eggs from liver and intestines. Liver was also used for DNA, protein, and collagen determinations. [³H] -Thymidine incorporation and collagen determinations were used to establish the indices given by the difference between their contents in infected and control animals and expressed per thousand eggs in liver. The recovery of worms in GF mice was around twice as much as in CV ones, and the total number of eggs was higher in the liver of GF animals. No hypertrophy of liver cells was observed by the ratio protein/DNA, but [³H]-thymidine incorporation into DNA was higher than in controls in both GF and CV infected animals. The [³H]-thymidine and collagen indices were lower in GF animals which indicate a more discrete cellular replication and smaller collagen content in relation to the number of eggs present in livers of these mice. It was concluded that the disease seems to be less severe in GF animals. |
format |
Article in Journal/Newspaper |
author |
Leda Q. Viera Tasso Moraes-santos |
author_facet |
Leda Q. Viera Tasso Moraes-santos |
author_sort |
Leda Q. Viera |
title |
Schistosomiasis mansoni: evidence for a milder response in germfree mice |
title_short |
Schistosomiasis mansoni: evidence for a milder response in germfree mice |
title_full |
Schistosomiasis mansoni: evidence for a milder response in germfree mice |
title_fullStr |
Schistosomiasis mansoni: evidence for a milder response in germfree mice |
title_full_unstemmed |
Schistosomiasis mansoni: evidence for a milder response in germfree mice |
title_sort |
schistosomiasis mansoni: evidence for a milder response in germfree mice |
publisher |
Universidade de São Paulo (USP) |
publishDate |
1987 |
url |
https://doaj.org/article/a8c01a8ea8a24aab8800d33547f5ebb2 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista do Instituto de Medicina Tropical de São Paulo, Vol 29, Iss 1, Pp 37-42 (1987) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0036-46651987000100006&tlng=en https://doaj.org/toc/1678-9946 1678-9946 https://doaj.org/article/a8c01a8ea8a24aab8800d33547f5ebb2 |
_version_ |
1809895784884207616 |