Toxinology in the proteomics era: a review on arachnid venom proteomics
Abstract The word venomics was coined to acknowledge the studies that use omics to investigate venom proteins and peptides. Venomics has evolved considerably over the last 20 years. The first works on scorpion or spider venomics were published in the early 2000’s. Such studies relied on peptide mass...
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ftdoajarticles:oai:doaj.org/article:a8bf5744f60c4679aa78cf4bcdfd6f25 2023-05-15T15:13:21+02:00 Toxinology in the proteomics era: a review on arachnid venom proteomics Filipi Calbaizer Marchi Edneia Mendes-Silva Lucas Rodrigues-Ribeiro Lucas Gabriel Bolais-Ramos Thiago Verano-Braga 2022-02-01T00:00:00Z https://doi.org/10.1590/1678-9199-jvatitd-2021-0034 https://doaj.org/article/a8bf5744f60c4679aa78cf4bcdfd6f25 EN eng SciELO http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992022000100201&lng=en&tlng=en http://www.scielo.br/pdf/jvatitd/v28/1678-9199-jvatitd-28-20210034.pdf https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2021-0034 https://doaj.org/article/a8bf5744f60c4679aa78cf4bcdfd6f25 Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 28 (2022) Proteomics Scorpions Spiders Venomics Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 article 2022 ftdoajarticles https://doi.org/10.1590/1678-9199-jvatitd-2021-0034 2022-12-31T07:32:24Z Abstract The word venomics was coined to acknowledge the studies that use omics to investigate venom proteins and peptides. Venomics has evolved considerably over the last 20 years. The first works on scorpion or spider venomics were published in the early 2000’s. Such studies relied on peptide mass fingerprinting (PMF) to characterize venom complexity. After the introduction of new mass spectrometers with higher resolution, sensitivity and mass accuracy, and the next-generation nucleotide sequencing, the complexity of data reported in research on scorpion and spider venomics increased exponentially, which allowed more comprehensive studies. In the present review article, we covered key publications on scorpion venomics and spider venomics, presenting historical grounds and implemented technologies over the last years. The literature presented in this review was selected after searching the PubMed database using the terms “(scorpion venom) AND (proteome)” for scorpion venomics, and “(spider venom) AND (proteome)” for publications on spider venomics. We presented the key aspects related to proteomics in the covered papers including, but not restricted to, the employed proteomic strategy (i.e., PMF, two-dimensional gel electrophoresis, shotgun/bottom-up and/or top-down/peptidome), and the type of mass spectrometer used. Some conclusions can be drawn from the present study. For example, the scorpion genus Tityus is the most studied concerning venomics, followed by Centruroides; whereas for spiders the studied genera were found more equally distributed. Another interesting conclusion is the lack of high throughput studies on post-translational modifications (PTMs) of scorpion and spider proteins. In our opinion, PTMs should be more studied as they can modulate the activity of scorpion and spider toxins. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Journal of Venomous Animals and Toxins including Tropical Diseases 28 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Proteomics Scorpions Spiders Venomics Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
spellingShingle |
Proteomics Scorpions Spiders Venomics Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 Filipi Calbaizer Marchi Edneia Mendes-Silva Lucas Rodrigues-Ribeiro Lucas Gabriel Bolais-Ramos Thiago Verano-Braga Toxinology in the proteomics era: a review on arachnid venom proteomics |
topic_facet |
Proteomics Scorpions Spiders Venomics Arctic medicine. Tropical medicine RC955-962 Toxicology. Poisons RA1190-1270 Zoology QL1-991 |
description |
Abstract The word venomics was coined to acknowledge the studies that use omics to investigate venom proteins and peptides. Venomics has evolved considerably over the last 20 years. The first works on scorpion or spider venomics were published in the early 2000’s. Such studies relied on peptide mass fingerprinting (PMF) to characterize venom complexity. After the introduction of new mass spectrometers with higher resolution, sensitivity and mass accuracy, and the next-generation nucleotide sequencing, the complexity of data reported in research on scorpion and spider venomics increased exponentially, which allowed more comprehensive studies. In the present review article, we covered key publications on scorpion venomics and spider venomics, presenting historical grounds and implemented technologies over the last years. The literature presented in this review was selected after searching the PubMed database using the terms “(scorpion venom) AND (proteome)” for scorpion venomics, and “(spider venom) AND (proteome)” for publications on spider venomics. We presented the key aspects related to proteomics in the covered papers including, but not restricted to, the employed proteomic strategy (i.e., PMF, two-dimensional gel electrophoresis, shotgun/bottom-up and/or top-down/peptidome), and the type of mass spectrometer used. Some conclusions can be drawn from the present study. For example, the scorpion genus Tityus is the most studied concerning venomics, followed by Centruroides; whereas for spiders the studied genera were found more equally distributed. Another interesting conclusion is the lack of high throughput studies on post-translational modifications (PTMs) of scorpion and spider proteins. In our opinion, PTMs should be more studied as they can modulate the activity of scorpion and spider toxins. |
format |
Article in Journal/Newspaper |
author |
Filipi Calbaizer Marchi Edneia Mendes-Silva Lucas Rodrigues-Ribeiro Lucas Gabriel Bolais-Ramos Thiago Verano-Braga |
author_facet |
Filipi Calbaizer Marchi Edneia Mendes-Silva Lucas Rodrigues-Ribeiro Lucas Gabriel Bolais-Ramos Thiago Verano-Braga |
author_sort |
Filipi Calbaizer Marchi |
title |
Toxinology in the proteomics era: a review on arachnid venom proteomics |
title_short |
Toxinology in the proteomics era: a review on arachnid venom proteomics |
title_full |
Toxinology in the proteomics era: a review on arachnid venom proteomics |
title_fullStr |
Toxinology in the proteomics era: a review on arachnid venom proteomics |
title_full_unstemmed |
Toxinology in the proteomics era: a review on arachnid venom proteomics |
title_sort |
toxinology in the proteomics era: a review on arachnid venom proteomics |
publisher |
SciELO |
publishDate |
2022 |
url |
https://doi.org/10.1590/1678-9199-jvatitd-2021-0034 https://doaj.org/article/a8bf5744f60c4679aa78cf4bcdfd6f25 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Journal of Venomous Animals and Toxins including Tropical Diseases, Vol 28 (2022) |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992022000100201&lng=en&tlng=en http://www.scielo.br/pdf/jvatitd/v28/1678-9199-jvatitd-28-20210034.pdf https://doaj.org/toc/1678-9199 1678-9199 doi:10.1590/1678-9199-jvatitd-2021-0034 https://doaj.org/article/a8bf5744f60c4679aa78cf4bcdfd6f25 |
op_doi |
https://doi.org/10.1590/1678-9199-jvatitd-2021-0034 |
container_title |
Journal of Venomous Animals and Toxins including Tropical Diseases |
container_volume |
28 |
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1766343908808196096 |