Genetic polymorphism of the N-terminal region in circumsporozoite surface protein of Plasmodium falciparum field isolates from Sudan
Abstract Background Malaria caused by Plasmodium falciparum parasite is still known to be one of the most significant public health problems in sub-Saharan Africa. Genetic diversity of the Sudanese P. falciparum based on the diversity in the circumsporozoite surface protein (PfCSP) has not been prev...
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ftdoajarticles:oai:doaj.org/article:a8acdd886211470ab5559d7cdcdcbf83 2023-05-15T15:17:29+02:00 Genetic polymorphism of the N-terminal region in circumsporozoite surface protein of Plasmodium falciparum field isolates from Sudan Nouh S. Mohamed Musab M. Ali Albsheer Hanadi Abdelbagi Emanuel E. Siddig Mona A. Mohamed Abdallah E. Ahmed Rihab Ali Omer Mohamed S. Muneer Ayman Ahmed Hussam A. Osman Mohamed S. Ali Ibrahim M. Eisa Mohamed M. Elbasheir 2019-10-01T00:00:00Z https://doi.org/10.1186/s12936-019-2970-0 https://doaj.org/article/a8acdd886211470ab5559d7cdcdcbf83 EN eng BMC http://link.springer.com/article/10.1186/s12936-019-2970-0 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-019-2970-0 1475-2875 https://doaj.org/article/a8acdd886211470ab5559d7cdcdcbf83 Malaria Journal, Vol 18, Iss 1, Pp 1-10 (2019) Plasmodium falciparum Circumsporozoite protein N-terminal region Genetic polymorphism Sudan Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2019 ftdoajarticles https://doi.org/10.1186/s12936-019-2970-0 2022-12-31T02:25:33Z Abstract Background Malaria caused by Plasmodium falciparum parasite is still known to be one of the most significant public health problems in sub-Saharan Africa. Genetic diversity of the Sudanese P. falciparum based on the diversity in the circumsporozoite surface protein (PfCSP) has not been previously studied. Therefore, this study aimed to investigate the genetic diversity of the N-terminal region of the pfcsp gene. Methods A cross-sectional molecular study was conducted; 50 blood samples have been analysed from different regions in Sudan. Patients were recruited from the health facilities of Khartoum, New Halfa, Red Sea, White Nile, Al Qadarif, Gezira, River Nile, and Ad Damazin during malaria transmission seasons between June to October and December to February 2017–2018. Microscopic and nested PCR was performed for detection of P. falciparum. Merozoite surface protein-1 was performed to differentiate single and multiple clonal infections. The N-terminal of the pfcsp gene has been sequenced using PCR-Sanger dideoxy method and analysed to sequences polymorphism including the numbers of haplotypes (H), segregating sites (S), haplotypes diversity (Hd) and the average number of nucleotide differences between two sequences (Pi) were obtained using the software DnaSP v5.10. As well as neutrality testing, Tajima’s D test, Fu and Li’s D and F statistics. Results PCR amplification resulted in 1200 bp of the pfcsp gene. Only 21 PCR products were successfully sequenced while 29 were presenting multiple clonal P. falciparum parasite were not sequenced. The analysis of the N-terminal region of the PfCSP amino acids sequence compared to the reference strains showed five different haplotypes. H1 consisted of 3D7, NF54, HB3 and 13 isolates of the Sudanese pfcsp. H2 comprised of 7G8, Dd2, MAD20, RO33, Wellcome strain, and 5 isolates of the Sudanese pfcsp. H3, H4, and H5 were found in 3 distinct isolates. Hd was 0.594 ± 0.065, and S was 12. The most common polymorphic site was A98G; other sites were D82Y, N83H, N83M, K85L, ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 18 1 |
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Plasmodium falciparum Circumsporozoite protein N-terminal region Genetic polymorphism Sudan Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Plasmodium falciparum Circumsporozoite protein N-terminal region Genetic polymorphism Sudan Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Nouh S. Mohamed Musab M. Ali Albsheer Hanadi Abdelbagi Emanuel E. Siddig Mona A. Mohamed Abdallah E. Ahmed Rihab Ali Omer Mohamed S. Muneer Ayman Ahmed Hussam A. Osman Mohamed S. Ali Ibrahim M. Eisa Mohamed M. Elbasheir Genetic polymorphism of the N-terminal region in circumsporozoite surface protein of Plasmodium falciparum field isolates from Sudan |
topic_facet |
Plasmodium falciparum Circumsporozoite protein N-terminal region Genetic polymorphism Sudan Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Malaria caused by Plasmodium falciparum parasite is still known to be one of the most significant public health problems in sub-Saharan Africa. Genetic diversity of the Sudanese P. falciparum based on the diversity in the circumsporozoite surface protein (PfCSP) has not been previously studied. Therefore, this study aimed to investigate the genetic diversity of the N-terminal region of the pfcsp gene. Methods A cross-sectional molecular study was conducted; 50 blood samples have been analysed from different regions in Sudan. Patients were recruited from the health facilities of Khartoum, New Halfa, Red Sea, White Nile, Al Qadarif, Gezira, River Nile, and Ad Damazin during malaria transmission seasons between June to October and December to February 2017–2018. Microscopic and nested PCR was performed for detection of P. falciparum. Merozoite surface protein-1 was performed to differentiate single and multiple clonal infections. The N-terminal of the pfcsp gene has been sequenced using PCR-Sanger dideoxy method and analysed to sequences polymorphism including the numbers of haplotypes (H), segregating sites (S), haplotypes diversity (Hd) and the average number of nucleotide differences between two sequences (Pi) were obtained using the software DnaSP v5.10. As well as neutrality testing, Tajima’s D test, Fu and Li’s D and F statistics. Results PCR amplification resulted in 1200 bp of the pfcsp gene. Only 21 PCR products were successfully sequenced while 29 were presenting multiple clonal P. falciparum parasite were not sequenced. The analysis of the N-terminal region of the PfCSP amino acids sequence compared to the reference strains showed five different haplotypes. H1 consisted of 3D7, NF54, HB3 and 13 isolates of the Sudanese pfcsp. H2 comprised of 7G8, Dd2, MAD20, RO33, Wellcome strain, and 5 isolates of the Sudanese pfcsp. H3, H4, and H5 were found in 3 distinct isolates. Hd was 0.594 ± 0.065, and S was 12. The most common polymorphic site was A98G; other sites were D82Y, N83H, N83M, K85L, ... |
format |
Article in Journal/Newspaper |
author |
Nouh S. Mohamed Musab M. Ali Albsheer Hanadi Abdelbagi Emanuel E. Siddig Mona A. Mohamed Abdallah E. Ahmed Rihab Ali Omer Mohamed S. Muneer Ayman Ahmed Hussam A. Osman Mohamed S. Ali Ibrahim M. Eisa Mohamed M. Elbasheir |
author_facet |
Nouh S. Mohamed Musab M. Ali Albsheer Hanadi Abdelbagi Emanuel E. Siddig Mona A. Mohamed Abdallah E. Ahmed Rihab Ali Omer Mohamed S. Muneer Ayman Ahmed Hussam A. Osman Mohamed S. Ali Ibrahim M. Eisa Mohamed M. Elbasheir |
author_sort |
Nouh S. Mohamed |
title |
Genetic polymorphism of the N-terminal region in circumsporozoite surface protein of Plasmodium falciparum field isolates from Sudan |
title_short |
Genetic polymorphism of the N-terminal region in circumsporozoite surface protein of Plasmodium falciparum field isolates from Sudan |
title_full |
Genetic polymorphism of the N-terminal region in circumsporozoite surface protein of Plasmodium falciparum field isolates from Sudan |
title_fullStr |
Genetic polymorphism of the N-terminal region in circumsporozoite surface protein of Plasmodium falciparum field isolates from Sudan |
title_full_unstemmed |
Genetic polymorphism of the N-terminal region in circumsporozoite surface protein of Plasmodium falciparum field isolates from Sudan |
title_sort |
genetic polymorphism of the n-terminal region in circumsporozoite surface protein of plasmodium falciparum field isolates from sudan |
publisher |
BMC |
publishDate |
2019 |
url |
https://doi.org/10.1186/s12936-019-2970-0 https://doaj.org/article/a8acdd886211470ab5559d7cdcdcbf83 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 18, Iss 1, Pp 1-10 (2019) |
op_relation |
http://link.springer.com/article/10.1186/s12936-019-2970-0 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-019-2970-0 1475-2875 https://doaj.org/article/a8acdd886211470ab5559d7cdcdcbf83 |
op_doi |
https://doi.org/10.1186/s12936-019-2970-0 |
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Malaria Journal |
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18 |
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1766347721255419904 |