Insight into protist diversity in Arctic sea ice and melt-pond aggregate obtained by pyrosequencing
Protists in the central Arctic Ocean are adapted to the harsh environmental conditions of its various habitats. During the Polarstern cruise ARK-XXVI/3 in 2011, at one sea-ice station, large aggregates accumulated at the bottom of the melt ponds. In this study, the protist assemblages of the bottom...
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Norwegian Polar Institute
2014
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ftdoajarticles:oai:doaj.org/article:a89d71c1604b47ee8ee182c8b44bd76c 2023-05-15T14:55:34+02:00 Insight into protist diversity in Arctic sea ice and melt-pond aggregate obtained by pyrosequencing Estelle Silvia Kilias Ilka Peeken Katja Metfies 2014-11-01T00:00:00Z https://doi.org/10.3402/polar.v33.23466 https://doaj.org/article/a89d71c1604b47ee8ee182c8b44bd76c EN eng Norwegian Polar Institute http://www.polarresearch.net/index.php/polar/article/download/23466/pdf_1 https://doaj.org/toc/1751-8369 1751-8369 doi:10.3402/polar.v33.23466 https://doaj.org/article/a89d71c1604b47ee8ee182c8b44bd76c Polar Research, Vol 33, Iss 0, Pp 1-10 (2014) 18S rDNA Arctic Ocean biodiversity next-generation sequencing flow cytometry Environmental sciences GE1-350 Oceanography GC1-1581 article 2014 ftdoajarticles https://doi.org/10.3402/polar.v33.23466 2022-12-31T02:19:05Z Protists in the central Arctic Ocean are adapted to the harsh environmental conditions of its various habitats. During the Polarstern cruise ARK-XXVI/3 in 2011, at one sea-ice station, large aggregates accumulated at the bottom of the melt ponds. In this study, the protist assemblages of the bottom layer of the sea-ice and melt-pond aggregate were investigated using flow cytometry and 454-pyrosequencing. The objective is to provide a first molecular overview of protist biodiversity in these habitats and to consider the overlaps and/or differences in the community compositions. Results of flow cytometry pointed to a cell size distribution that was dominated by 3–10 µm nanoflagellates. The phylogenetic classification of all sequences was conducted at a high taxonomic level, while a selection of abundant (≥1% of total reads) sequences was further classified at a lower level. At a high taxonomic level, both habitats showed very similar community structures, dominated by chrysophytes and chlorophytes. At a lower taxonomic level, dissimilarities in the diversity of both groups were encountered in the abundant biosphere. While sea-ice chlorophytes and chrysophytes were dominated by Chlamydomonas/Chloromonas spp. and Ochromonas spp., the melt-pond aggregate was dominated by Carteria sp., Ochromonas spp. and Dinobryon faculiferum. We suppose that the similarities in richness and community structure are a consequence of melt-pond freshwater seeping through porous sea ice in late summer. Differences in the abundant biosphere nevertheless indicate that environmental conditions in both habitats vary enough to select for different dominant species. Article in Journal/Newspaper Arctic Arctic Ocean Polar Research Sea ice Directory of Open Access Journals: DOAJ Articles Arctic Arctic Ocean Polar Research 33 1 23466 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
18S rDNA Arctic Ocean biodiversity next-generation sequencing flow cytometry Environmental sciences GE1-350 Oceanography GC1-1581 |
spellingShingle |
18S rDNA Arctic Ocean biodiversity next-generation sequencing flow cytometry Environmental sciences GE1-350 Oceanography GC1-1581 Estelle Silvia Kilias Ilka Peeken Katja Metfies Insight into protist diversity in Arctic sea ice and melt-pond aggregate obtained by pyrosequencing |
topic_facet |
18S rDNA Arctic Ocean biodiversity next-generation sequencing flow cytometry Environmental sciences GE1-350 Oceanography GC1-1581 |
description |
Protists in the central Arctic Ocean are adapted to the harsh environmental conditions of its various habitats. During the Polarstern cruise ARK-XXVI/3 in 2011, at one sea-ice station, large aggregates accumulated at the bottom of the melt ponds. In this study, the protist assemblages of the bottom layer of the sea-ice and melt-pond aggregate were investigated using flow cytometry and 454-pyrosequencing. The objective is to provide a first molecular overview of protist biodiversity in these habitats and to consider the overlaps and/or differences in the community compositions. Results of flow cytometry pointed to a cell size distribution that was dominated by 3–10 µm nanoflagellates. The phylogenetic classification of all sequences was conducted at a high taxonomic level, while a selection of abundant (≥1% of total reads) sequences was further classified at a lower level. At a high taxonomic level, both habitats showed very similar community structures, dominated by chrysophytes and chlorophytes. At a lower taxonomic level, dissimilarities in the diversity of both groups were encountered in the abundant biosphere. While sea-ice chlorophytes and chrysophytes were dominated by Chlamydomonas/Chloromonas spp. and Ochromonas spp., the melt-pond aggregate was dominated by Carteria sp., Ochromonas spp. and Dinobryon faculiferum. We suppose that the similarities in richness and community structure are a consequence of melt-pond freshwater seeping through porous sea ice in late summer. Differences in the abundant biosphere nevertheless indicate that environmental conditions in both habitats vary enough to select for different dominant species. |
format |
Article in Journal/Newspaper |
author |
Estelle Silvia Kilias Ilka Peeken Katja Metfies |
author_facet |
Estelle Silvia Kilias Ilka Peeken Katja Metfies |
author_sort |
Estelle Silvia Kilias |
title |
Insight into protist diversity in Arctic sea ice and melt-pond aggregate obtained by pyrosequencing |
title_short |
Insight into protist diversity in Arctic sea ice and melt-pond aggregate obtained by pyrosequencing |
title_full |
Insight into protist diversity in Arctic sea ice and melt-pond aggregate obtained by pyrosequencing |
title_fullStr |
Insight into protist diversity in Arctic sea ice and melt-pond aggregate obtained by pyrosequencing |
title_full_unstemmed |
Insight into protist diversity in Arctic sea ice and melt-pond aggregate obtained by pyrosequencing |
title_sort |
insight into protist diversity in arctic sea ice and melt-pond aggregate obtained by pyrosequencing |
publisher |
Norwegian Polar Institute |
publishDate |
2014 |
url |
https://doi.org/10.3402/polar.v33.23466 https://doaj.org/article/a89d71c1604b47ee8ee182c8b44bd76c |
geographic |
Arctic Arctic Ocean |
geographic_facet |
Arctic Arctic Ocean |
genre |
Arctic Arctic Ocean Polar Research Sea ice |
genre_facet |
Arctic Arctic Ocean Polar Research Sea ice |
op_source |
Polar Research, Vol 33, Iss 0, Pp 1-10 (2014) |
op_relation |
http://www.polarresearch.net/index.php/polar/article/download/23466/pdf_1 https://doaj.org/toc/1751-8369 1751-8369 doi:10.3402/polar.v33.23466 https://doaj.org/article/a89d71c1604b47ee8ee182c8b44bd76c |
op_doi |
https://doi.org/10.3402/polar.v33.23466 |
container_title |
Polar Research |
container_volume |
33 |
container_issue |
1 |
container_start_page |
23466 |
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1766327595809374208 |