Identification and partial purification of an anticoagulant factor from the venom of the Iranian snake Agkistrodon halys

• Main Authors: M Ghorbanpur, A Zare Mirakabadi, F Zokaee, H Zolfagarrian
• Format: Article in Journal/Newspaper
• Language: English
• Published: SciELO 2010
• Subjects: snake venom; Agkistrodon halys; anticoagulant factor; chromatography; Arctic medicine. Tropical medicine; RC955-962; Toxicology. Poisons; RA1190-1270; Zoology; QL1-991; Arctic
• Online Access: https://doaj.org/article/a8429dfe30e849b485a99f2fc39b0d4f

An anticoagulant factor was purified from the venom of the Iranian snake Agkistrodon halys by gel filtration on Sephadex G-50 and ion-exchange chromatography on DEAE-Sepharose. In the final stage of purification, the percentage recovery of purified anticoagulant factor was found to be 83%. The purified anticoagulant factor revealed a single protein band in SDS-polyacrylamide electrophoresis under reducing conditions and its molecular weight was about 22 kDa. The purified peptide did not show any effect on casein, BApNA or plasma.