Fine analysis of genetic diversity of the tpr gene family among treponemal species, subspecies and strains.
The pathogenic non-cultivable treponemes include three subspecies of Treponema pallidum (pallidum, pertenue, endemicum), T. carateum, T. paraluiscuniculi, and the unclassified Fribourg-Blanc treponeme (Simian isolate). These treponemes are morphologically indistinguishable and antigenically and gene...
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ftdoajarticles:oai:doaj.org/article:a7e499ca084349a89c8f0a2d6aef692b 2023-05-15T15:14:21+02:00 Fine analysis of genetic diversity of the tpr gene family among treponemal species, subspecies and strains. Arturo Centurion-Lara Lorenzo Giacani Charmie Godornes Barbara J Molini Tara Brinck Reid Sheila A Lukehart 2013-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0002222 https://doaj.org/article/a7e499ca084349a89c8f0a2d6aef692b EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3656149?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002222 https://doaj.org/article/a7e499ca084349a89c8f0a2d6aef692b PLoS Neglected Tropical Diseases, Vol 7, Iss 5, p e2222 (2013) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2013 ftdoajarticles https://doi.org/10.1371/journal.pntd.0002222 2022-12-31T01:32:52Z The pathogenic non-cultivable treponemes include three subspecies of Treponema pallidum (pallidum, pertenue, endemicum), T. carateum, T. paraluiscuniculi, and the unclassified Fribourg-Blanc treponeme (Simian isolate). These treponemes are morphologically indistinguishable and antigenically and genetically highly similar, yet cross-immunity is variable or non-existent. Although all of these organisms cause chronic, multistage skin and systemic disease, they have historically been classified by mode of transmission, clinical presentations and host ranges. Whole genome studies underscore the high degree of sequence identity among species, subspecies and strains, pinpointing a limited number of genomic regions for variation. Many of these "hot spots" include members of the tpr gene family, composed of 12 paralogs encoding candidate virulence factors. We hypothesize that the distinct clinical presentations, host specificity, and variable cross-immunity might reside on virulence factors such as the tpr genes.Sequence analysis of 11 tpr loci (excluding tprK) from 12 strains demonstrated an impressive heterogeneity, including SNPs, indels, chimeric genes, truncated gene products and large deletions. Comparative analyses of sequences and 3D models of predicted proteins in Subfamily I highlight the striking co-localization of discrete variable regions with predicted surface-exposed loops. A hallmark of Subfamily II is the presence of chimeric genes in the tprG and J loci. Diversity in Subfamily III is limited to tprA and tprL.An impressive sequence variability was found in tpr sequences among the Treponema isolates examined in this study, with most of the variation being consistent within subspecies or species, or between syphilis vs. non-syphilis strains. Variability was seen in the pallidum subspecies, which can be divided into 5 genogroups. These findings support a genetic basis for the classification of these organisms into their respective subspecies and species. Future functional studies will determine whether the ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 7 5 e2222 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Arturo Centurion-Lara Lorenzo Giacani Charmie Godornes Barbara J Molini Tara Brinck Reid Sheila A Lukehart Fine analysis of genetic diversity of the tpr gene family among treponemal species, subspecies and strains. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
The pathogenic non-cultivable treponemes include three subspecies of Treponema pallidum (pallidum, pertenue, endemicum), T. carateum, T. paraluiscuniculi, and the unclassified Fribourg-Blanc treponeme (Simian isolate). These treponemes are morphologically indistinguishable and antigenically and genetically highly similar, yet cross-immunity is variable or non-existent. Although all of these organisms cause chronic, multistage skin and systemic disease, they have historically been classified by mode of transmission, clinical presentations and host ranges. Whole genome studies underscore the high degree of sequence identity among species, subspecies and strains, pinpointing a limited number of genomic regions for variation. Many of these "hot spots" include members of the tpr gene family, composed of 12 paralogs encoding candidate virulence factors. We hypothesize that the distinct clinical presentations, host specificity, and variable cross-immunity might reside on virulence factors such as the tpr genes.Sequence analysis of 11 tpr loci (excluding tprK) from 12 strains demonstrated an impressive heterogeneity, including SNPs, indels, chimeric genes, truncated gene products and large deletions. Comparative analyses of sequences and 3D models of predicted proteins in Subfamily I highlight the striking co-localization of discrete variable regions with predicted surface-exposed loops. A hallmark of Subfamily II is the presence of chimeric genes in the tprG and J loci. Diversity in Subfamily III is limited to tprA and tprL.An impressive sequence variability was found in tpr sequences among the Treponema isolates examined in this study, with most of the variation being consistent within subspecies or species, or between syphilis vs. non-syphilis strains. Variability was seen in the pallidum subspecies, which can be divided into 5 genogroups. These findings support a genetic basis for the classification of these organisms into their respective subspecies and species. Future functional studies will determine whether the ... |
format |
Article in Journal/Newspaper |
author |
Arturo Centurion-Lara Lorenzo Giacani Charmie Godornes Barbara J Molini Tara Brinck Reid Sheila A Lukehart |
author_facet |
Arturo Centurion-Lara Lorenzo Giacani Charmie Godornes Barbara J Molini Tara Brinck Reid Sheila A Lukehart |
author_sort |
Arturo Centurion-Lara |
title |
Fine analysis of genetic diversity of the tpr gene family among treponemal species, subspecies and strains. |
title_short |
Fine analysis of genetic diversity of the tpr gene family among treponemal species, subspecies and strains. |
title_full |
Fine analysis of genetic diversity of the tpr gene family among treponemal species, subspecies and strains. |
title_fullStr |
Fine analysis of genetic diversity of the tpr gene family among treponemal species, subspecies and strains. |
title_full_unstemmed |
Fine analysis of genetic diversity of the tpr gene family among treponemal species, subspecies and strains. |
title_sort |
fine analysis of genetic diversity of the tpr gene family among treponemal species, subspecies and strains. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2013 |
url |
https://doi.org/10.1371/journal.pntd.0002222 https://doaj.org/article/a7e499ca084349a89c8f0a2d6aef692b |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 7, Iss 5, p e2222 (2013) |
op_relation |
http://europepmc.org/articles/PMC3656149?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0002222 https://doaj.org/article/a7e499ca084349a89c8f0a2d6aef692b |
op_doi |
https://doi.org/10.1371/journal.pntd.0002222 |
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PLoS Neglected Tropical Diseases |
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7 |
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5 |
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e2222 |
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1766344811395153920 |