BMP7 Functions to Regulate Proliferation of Dermal Papilla Cells in Hu Sheep
Bone morphogenetic proteins (BMPs) are the structurally similar and highly conserved type of functional proteins that play an important role in hair follicle growth and development. BMP7 was a differentially expressed gene in different patterns of Hu sheep lambskin identified using Agilent microarra...
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ftdoajarticles:oai:doaj.org/article:a7d724c275a14b25a81b0e2a324d2b73 2023-05-15T15:51:21+02:00 BMP7 Functions to Regulate Proliferation of Dermal Papilla Cells in Hu Sheep Yue Li Xiaoyang Lv Shanhe Wang Xiukai Cao Zehu Yuan Tesfaye Getachew Joram M. Mwacharo Aynalem Haile Wei Sun 2022-01-01T00:00:00Z https://doi.org/10.3390/genes13020201 https://doaj.org/article/a7d724c275a14b25a81b0e2a324d2b73 EN eng MDPI AG https://www.mdpi.com/2073-4425/13/2/201 https://doaj.org/toc/2073-4425 doi:10.3390/genes13020201 2073-4425 https://doaj.org/article/a7d724c275a14b25a81b0e2a324d2b73 Genes, Vol 13, Iss 201, p 201 (2022) BMP7 Hu sheep dermal papilla cells cell proliferation Genetics QH426-470 article 2022 ftdoajarticles https://doi.org/10.3390/genes13020201 2022-12-31T16:02:03Z Bone morphogenetic proteins (BMPs) are the structurally similar and highly conserved type of functional proteins that play an important role in hair follicle growth and development. BMP7 was a differentially expressed gene in different patterns of Hu sheep lambskin identified using Agilent microarray. Since hair follicle is the basis of pattern formation of lambskin, and its growth and development is governed by dermal papilla cells (DPCs), to clarify the role of BMP7 and hair follicle, our study was designed to investigate the regulation between BMP7 and DPCs. Firstly, the CDS region of BMP7 was cloned by 3’Race and PCR in Hu sheep and performed serious of bioinformatic analysis. Then, the effects of BMP7 on DPCs were analyzed after overexpression and interference of BMP7 in dermal papilla cells by CCK8, EdU, and PI assay. Additionally, qPCR was also conducted to clarify the relationship between BMP7 and the TGF-β/Smad signaling pathway. A total of 1296 bp of the BMP7 CDS region sequence was sucessfully cloned in Hu sheep, encoding a signal peptide of 431 amino acids, molecular weight was 49,316.9 Da and the isoelectric point (Pi) was 7.75. Nucleotide sequencing analysis of BMP7 revealed that Hu sheep had high homology with Bos taurus, Homo sapiens, and Canis lupus familiaris . Structure domain prediction showed that TGF-β superfamily domain exist between 330th–431th amino acid, BMP7 protein is a secreted protein. In BMP7 up-regulated DPCs, DPCs proliferation rate and cell cycle were significantly higher than that of NC group ( p < 0.05). Meanwhile, the expression level of Smad3 , Smad4 , Samd6 , and TGF-β1 in TGF-β/Smad signaling pathway were significantly lower than that in NC group ( p < 0.05). In BMP7 down-regulated DPCs, it presented the opposite result. In conclusion, our study showed that BMP7 had a positive effect on DPCs by accelerating the proliferation and cell cycle of DPCs, and hypothesized that regulate hair follicles growth and development via TGF-β/Smad signaling pathway. These findings ... Article in Journal/Newspaper Canis lupus Directory of Open Access Journals: DOAJ Articles Genes 13 2 201 |
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ftdoajarticles |
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English |
topic |
BMP7 Hu sheep dermal papilla cells cell proliferation Genetics QH426-470 |
spellingShingle |
BMP7 Hu sheep dermal papilla cells cell proliferation Genetics QH426-470 Yue Li Xiaoyang Lv Shanhe Wang Xiukai Cao Zehu Yuan Tesfaye Getachew Joram M. Mwacharo Aynalem Haile Wei Sun BMP7 Functions to Regulate Proliferation of Dermal Papilla Cells in Hu Sheep |
topic_facet |
BMP7 Hu sheep dermal papilla cells cell proliferation Genetics QH426-470 |
description |
Bone morphogenetic proteins (BMPs) are the structurally similar and highly conserved type of functional proteins that play an important role in hair follicle growth and development. BMP7 was a differentially expressed gene in different patterns of Hu sheep lambskin identified using Agilent microarray. Since hair follicle is the basis of pattern formation of lambskin, and its growth and development is governed by dermal papilla cells (DPCs), to clarify the role of BMP7 and hair follicle, our study was designed to investigate the regulation between BMP7 and DPCs. Firstly, the CDS region of BMP7 was cloned by 3’Race and PCR in Hu sheep and performed serious of bioinformatic analysis. Then, the effects of BMP7 on DPCs were analyzed after overexpression and interference of BMP7 in dermal papilla cells by CCK8, EdU, and PI assay. Additionally, qPCR was also conducted to clarify the relationship between BMP7 and the TGF-β/Smad signaling pathway. A total of 1296 bp of the BMP7 CDS region sequence was sucessfully cloned in Hu sheep, encoding a signal peptide of 431 amino acids, molecular weight was 49,316.9 Da and the isoelectric point (Pi) was 7.75. Nucleotide sequencing analysis of BMP7 revealed that Hu sheep had high homology with Bos taurus, Homo sapiens, and Canis lupus familiaris . Structure domain prediction showed that TGF-β superfamily domain exist between 330th–431th amino acid, BMP7 protein is a secreted protein. In BMP7 up-regulated DPCs, DPCs proliferation rate and cell cycle were significantly higher than that of NC group ( p < 0.05). Meanwhile, the expression level of Smad3 , Smad4 , Samd6 , and TGF-β1 in TGF-β/Smad signaling pathway were significantly lower than that in NC group ( p < 0.05). In BMP7 down-regulated DPCs, it presented the opposite result. In conclusion, our study showed that BMP7 had a positive effect on DPCs by accelerating the proliferation and cell cycle of DPCs, and hypothesized that regulate hair follicles growth and development via TGF-β/Smad signaling pathway. These findings ... |
format |
Article in Journal/Newspaper |
author |
Yue Li Xiaoyang Lv Shanhe Wang Xiukai Cao Zehu Yuan Tesfaye Getachew Joram M. Mwacharo Aynalem Haile Wei Sun |
author_facet |
Yue Li Xiaoyang Lv Shanhe Wang Xiukai Cao Zehu Yuan Tesfaye Getachew Joram M. Mwacharo Aynalem Haile Wei Sun |
author_sort |
Yue Li |
title |
BMP7 Functions to Regulate Proliferation of Dermal Papilla Cells in Hu Sheep |
title_short |
BMP7 Functions to Regulate Proliferation of Dermal Papilla Cells in Hu Sheep |
title_full |
BMP7 Functions to Regulate Proliferation of Dermal Papilla Cells in Hu Sheep |
title_fullStr |
BMP7 Functions to Regulate Proliferation of Dermal Papilla Cells in Hu Sheep |
title_full_unstemmed |
BMP7 Functions to Regulate Proliferation of Dermal Papilla Cells in Hu Sheep |
title_sort |
bmp7 functions to regulate proliferation of dermal papilla cells in hu sheep |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/genes13020201 https://doaj.org/article/a7d724c275a14b25a81b0e2a324d2b73 |
genre |
Canis lupus |
genre_facet |
Canis lupus |
op_source |
Genes, Vol 13, Iss 201, p 201 (2022) |
op_relation |
https://www.mdpi.com/2073-4425/13/2/201 https://doaj.org/toc/2073-4425 doi:10.3390/genes13020201 2073-4425 https://doaj.org/article/a7d724c275a14b25a81b0e2a324d2b73 |
op_doi |
https://doi.org/10.3390/genes13020201 |
container_title |
Genes |
container_volume |
13 |
container_issue |
2 |
container_start_page |
201 |
_version_ |
1766386536295694336 |