Faecal Parasitology: Concentration Methodology Needs to be Better Standardised.
To determine whether variation in the preservative, pore size of the sieve, solvent, centrifugal force and centrifugation time used in the Ridley-Allen Concentration method for examining faecal specimens for parasite stages had any effect on their recovery in faecal specimens.A questionnaire was sen...
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ftdoajarticles:oai:doaj.org/article:a7ca9544fd0743c28acc476a495b8a47 2023-05-15T15:06:17+02:00 Faecal Parasitology: Concentration Methodology Needs to be Better Standardised. Monika M Manser Agatha Christie Santos Saez Peter L Chiodini 2016-04-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0004579 https://doaj.org/article/a7ca9544fd0743c28acc476a495b8a47 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4830587?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004579 https://doaj.org/article/a7ca9544fd0743c28acc476a495b8a47 PLoS Neglected Tropical Diseases, Vol 10, Iss 4, p e0004579 (2016) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2016 ftdoajarticles https://doi.org/10.1371/journal.pntd.0004579 2022-12-31T01:35:42Z To determine whether variation in the preservative, pore size of the sieve, solvent, centrifugal force and centrifugation time used in the Ridley-Allen Concentration method for examining faecal specimens for parasite stages had any effect on their recovery in faecal specimens.A questionnaire was sent to all participants in the UK NEQAS Faecal Parasitology Scheme. The recovery of parasite stages was compared using formalin diluted in water or formalin diluted in saline as the fixative, 3 different pore sizes of sieve, ether or ethyl acetate as a solvent, 7 different centrifugal forces and 6 different centrifugation times according to the methods described by participants completing the questionnaire.The number of parasite stages recovered was higher when formalin diluted in water was used as fixative, a smaller pore size of sieve was used, ethyl acetate along with Triton X 100 was used as a solvent and a centrifugal force of 3,000 rpm for 3 minutes were employed.This study showed that differences in methodology at various stages of the concentration process affect the recovery of parasites from a faecal specimen and parasites present in small numbers could be missed if the recommended methodology is not followed. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Ridley ENVELOPE(-130.315,-130.315,54.250,54.250) Triton ENVELOPE(-55.615,-55.615,49.517,49.517) PLOS Neglected Tropical Diseases 10 4 e0004579 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Monika M Manser Agatha Christie Santos Saez Peter L Chiodini Faecal Parasitology: Concentration Methodology Needs to be Better Standardised. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
To determine whether variation in the preservative, pore size of the sieve, solvent, centrifugal force and centrifugation time used in the Ridley-Allen Concentration method for examining faecal specimens for parasite stages had any effect on their recovery in faecal specimens.A questionnaire was sent to all participants in the UK NEQAS Faecal Parasitology Scheme. The recovery of parasite stages was compared using formalin diluted in water or formalin diluted in saline as the fixative, 3 different pore sizes of sieve, ether or ethyl acetate as a solvent, 7 different centrifugal forces and 6 different centrifugation times according to the methods described by participants completing the questionnaire.The number of parasite stages recovered was higher when formalin diluted in water was used as fixative, a smaller pore size of sieve was used, ethyl acetate along with Triton X 100 was used as a solvent and a centrifugal force of 3,000 rpm for 3 minutes were employed.This study showed that differences in methodology at various stages of the concentration process affect the recovery of parasites from a faecal specimen and parasites present in small numbers could be missed if the recommended methodology is not followed. |
format |
Article in Journal/Newspaper |
author |
Monika M Manser Agatha Christie Santos Saez Peter L Chiodini |
author_facet |
Monika M Manser Agatha Christie Santos Saez Peter L Chiodini |
author_sort |
Monika M Manser |
title |
Faecal Parasitology: Concentration Methodology Needs to be Better Standardised. |
title_short |
Faecal Parasitology: Concentration Methodology Needs to be Better Standardised. |
title_full |
Faecal Parasitology: Concentration Methodology Needs to be Better Standardised. |
title_fullStr |
Faecal Parasitology: Concentration Methodology Needs to be Better Standardised. |
title_full_unstemmed |
Faecal Parasitology: Concentration Methodology Needs to be Better Standardised. |
title_sort |
faecal parasitology: concentration methodology needs to be better standardised. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pntd.0004579 https://doaj.org/article/a7ca9544fd0743c28acc476a495b8a47 |
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ENVELOPE(-130.315,-130.315,54.250,54.250) ENVELOPE(-55.615,-55.615,49.517,49.517) |
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Arctic Ridley Triton |
geographic_facet |
Arctic Ridley Triton |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 10, Iss 4, p e0004579 (2016) |
op_relation |
http://europepmc.org/articles/PMC4830587?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004579 https://doaj.org/article/a7ca9544fd0743c28acc476a495b8a47 |
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https://doi.org/10.1371/journal.pntd.0004579 |
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PLOS Neglected Tropical Diseases |
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e0004579 |
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