Effects of in vitro exposure to diarrheic toxin producer Prorocentrum lima on gene expressions related to cell cycle regulation and immune response in Crassostrea gigas.
BACKGROUND: Crassostrea gigas accumulates diarrheic shellfish toxins (DSP) associated to Prorocentrum lima of which Okadaic acid (OA) causes specific inhibitions of serine and threonine phosphatases 1 and 2A. Its toxic effects have been extensively reported in bivalve mollusks at cellular and physio...
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ftdoajarticles:oai:doaj.org/article:a7c56c6538ab4460bd4d77c317c039a9 2023-05-15T15:58:25+02:00 Effects of in vitro exposure to diarrheic toxin producer Prorocentrum lima on gene expressions related to cell cycle regulation and immune response in Crassostrea gigas. Reyna de Jesús Romero-Geraldo Norma García-Lagunas Norma Yolanda Hernández-Saavedra 2014-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0097181 https://doaj.org/article/a7c56c6538ab4460bd4d77c317c039a9 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4019545?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0097181 https://doaj.org/article/a7c56c6538ab4460bd4d77c317c039a9 PLoS ONE, Vol 9, Iss 5, p e97181 (2014) Medicine R Science Q article 2014 ftdoajarticles https://doi.org/10.1371/journal.pone.0097181 2022-12-31T10:16:02Z BACKGROUND: Crassostrea gigas accumulates diarrheic shellfish toxins (DSP) associated to Prorocentrum lima of which Okadaic acid (OA) causes specific inhibitions of serine and threonine phosphatases 1 and 2A. Its toxic effects have been extensively reported in bivalve mollusks at cellular and physiological levels, but genomic approaches have been scarcely studied. METHODOLOGY/PRINCIPAL FINDINGS: Acute and sub-chronic exposure effects of P. lima were investigated on farmed juvenile C. gigas (3-5 mm). The Pacific oysters were fed with three dinoflagellate concentrations: 0.3, 3, and 30 ×10(3) cells mL-1 along with a nontoxic control diet of Isochrysis galbana. The effects of P. lima on C. gigas were followed by analyzing expression levels of a total of four genes, three involved in cell cycle regulation and one in immune response by polymerase chain reaction and real time quantitative PCR, where changes in time and cell concentration were found. The highest expression levels were found in oysters fed 3 × 10(3) cells mL-1 at 168 h for the cycle regulator p21 protein (9 fold), chromatin assembly factor 1 p55 subunit (8 fold), elongation factor 2 (2 fold), and lipopolysaccharide/β-1, 3 glucan binding protein (13 fold above base line). Additionally, the transcript level of all the genes decreased in oysters fed wich the mixed diet 30 × 10(3) cells mL-1 of dinoflagellate after 72 h and was lowest in the chromatin assembly factor 1 p55 subunit (0.9 fold below baseline). CONCLUSIONS: On C. gigas the whole cell ingestion of P lima caused a clear mRNA modulation expression of the genes involved in cell cycle regulation and immune system. Over-expression could be related to DNA damage, disturbances in cell cycle continuity, probably a genotoxic effect, as well as an activation of its innate immune system as first line of defense. Article in Journal/Newspaper Crassostrea gigas Directory of Open Access Journals: DOAJ Articles Pacific PLoS ONE 9 5 e97181 |
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English |
topic |
Medicine R Science Q |
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Medicine R Science Q Reyna de Jesús Romero-Geraldo Norma García-Lagunas Norma Yolanda Hernández-Saavedra Effects of in vitro exposure to diarrheic toxin producer Prorocentrum lima on gene expressions related to cell cycle regulation and immune response in Crassostrea gigas. |
topic_facet |
Medicine R Science Q |
description |
BACKGROUND: Crassostrea gigas accumulates diarrheic shellfish toxins (DSP) associated to Prorocentrum lima of which Okadaic acid (OA) causes specific inhibitions of serine and threonine phosphatases 1 and 2A. Its toxic effects have been extensively reported in bivalve mollusks at cellular and physiological levels, but genomic approaches have been scarcely studied. METHODOLOGY/PRINCIPAL FINDINGS: Acute and sub-chronic exposure effects of P. lima were investigated on farmed juvenile C. gigas (3-5 mm). The Pacific oysters were fed with three dinoflagellate concentrations: 0.3, 3, and 30 ×10(3) cells mL-1 along with a nontoxic control diet of Isochrysis galbana. The effects of P. lima on C. gigas were followed by analyzing expression levels of a total of four genes, three involved in cell cycle regulation and one in immune response by polymerase chain reaction and real time quantitative PCR, where changes in time and cell concentration were found. The highest expression levels were found in oysters fed 3 × 10(3) cells mL-1 at 168 h for the cycle regulator p21 protein (9 fold), chromatin assembly factor 1 p55 subunit (8 fold), elongation factor 2 (2 fold), and lipopolysaccharide/β-1, 3 glucan binding protein (13 fold above base line). Additionally, the transcript level of all the genes decreased in oysters fed wich the mixed diet 30 × 10(3) cells mL-1 of dinoflagellate after 72 h and was lowest in the chromatin assembly factor 1 p55 subunit (0.9 fold below baseline). CONCLUSIONS: On C. gigas the whole cell ingestion of P lima caused a clear mRNA modulation expression of the genes involved in cell cycle regulation and immune system. Over-expression could be related to DNA damage, disturbances in cell cycle continuity, probably a genotoxic effect, as well as an activation of its innate immune system as first line of defense. |
format |
Article in Journal/Newspaper |
author |
Reyna de Jesús Romero-Geraldo Norma García-Lagunas Norma Yolanda Hernández-Saavedra |
author_facet |
Reyna de Jesús Romero-Geraldo Norma García-Lagunas Norma Yolanda Hernández-Saavedra |
author_sort |
Reyna de Jesús Romero-Geraldo |
title |
Effects of in vitro exposure to diarrheic toxin producer Prorocentrum lima on gene expressions related to cell cycle regulation and immune response in Crassostrea gigas. |
title_short |
Effects of in vitro exposure to diarrheic toxin producer Prorocentrum lima on gene expressions related to cell cycle regulation and immune response in Crassostrea gigas. |
title_full |
Effects of in vitro exposure to diarrheic toxin producer Prorocentrum lima on gene expressions related to cell cycle regulation and immune response in Crassostrea gigas. |
title_fullStr |
Effects of in vitro exposure to diarrheic toxin producer Prorocentrum lima on gene expressions related to cell cycle regulation and immune response in Crassostrea gigas. |
title_full_unstemmed |
Effects of in vitro exposure to diarrheic toxin producer Prorocentrum lima on gene expressions related to cell cycle regulation and immune response in Crassostrea gigas. |
title_sort |
effects of in vitro exposure to diarrheic toxin producer prorocentrum lima on gene expressions related to cell cycle regulation and immune response in crassostrea gigas. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2014 |
url |
https://doi.org/10.1371/journal.pone.0097181 https://doaj.org/article/a7c56c6538ab4460bd4d77c317c039a9 |
geographic |
Pacific |
geographic_facet |
Pacific |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
PLoS ONE, Vol 9, Iss 5, p e97181 (2014) |
op_relation |
http://europepmc.org/articles/PMC4019545?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0097181 https://doaj.org/article/a7c56c6538ab4460bd4d77c317c039a9 |
op_doi |
https://doi.org/10.1371/journal.pone.0097181 |
container_title |
PLoS ONE |
container_volume |
9 |
container_issue |
5 |
container_start_page |
e97181 |
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