Molecular surveillance of piroplasms in ticks from small and medium-sized urban and peri-urban mammals in Australia
Natural landscape alterations as a consequence of urbanisation are one of the main drivers in the movements of wildlife into metropolitan and peri-urban areas. Worldwide, these wildlife species are highly adaptable and may be responsible for the transmission of tick-borne pathogens including piropla...
Published in: | International Journal for Parasitology: Parasites and Wildlife |
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Online Access: | https://doi.org/10.1016/j.ijppaw.2018.05.005 https://doaj.org/article/a7c11bc51d124245a6390a742f21eac3 |
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ftdoajarticles:oai:doaj.org/article:a7c11bc51d124245a6390a742f21eac3 2023-05-15T18:05:39+02:00 Molecular surveillance of piroplasms in ticks from small and medium-sized urban and peri-urban mammals in Australia Siew-May Loh Siobhon Egan Amber Gillett Peter B. Banks Una M. Ryan Peter J. Irwin Charlotte L. Oskam 2018-08-01T00:00:00Z https://doi.org/10.1016/j.ijppaw.2018.05.005 https://doaj.org/article/a7c11bc51d124245a6390a742f21eac3 EN eng Elsevier http://www.sciencedirect.com/science/article/pii/S2213224418300166 https://doaj.org/toc/2213-2244 2213-2244 doi:10.1016/j.ijppaw.2018.05.005 https://doaj.org/article/a7c11bc51d124245a6390a742f21eac3 International Journal for Parasitology: Parasites and Wildlife, Vol 7, Iss 2, Pp 197-203 (2018) Zoology QL1-991 article 2018 ftdoajarticles https://doi.org/10.1016/j.ijppaw.2018.05.005 2022-12-31T01:49:25Z Natural landscape alterations as a consequence of urbanisation are one of the main drivers in the movements of wildlife into metropolitan and peri-urban areas. Worldwide, these wildlife species are highly adaptable and may be responsible for the transmission of tick-borne pathogens including piroplasms (Babesia, Theileria and Cytauxzoon spp.) that cause piroplasmosis in animals and occasionally in humans. Little is known about piroplasms in the ticks of urban wildlife in Australia. Ticks from long-nosed bandicoots (Perameles nasuta; n = 71), eastern-barred bandicoots (Perameles gunnii; n = 41), northern-brown bandicoots (Isoodon macrourus; n = 19), southern-brown bandicoots (Isoodon obesulus; n = 4), bandicoot sp. (n = 2), flying foxes (Pteropus sp.; n = 3), black rats (Rattus rattus; n = 7), bush rats (Rattus fuscipes; n = 4), brushtail possums (Trichosurus vulpecula; n = 19), ringtail possums (Pseudocheirus peregrinus; n = 12), short-eared possums (Trichosurus caninus; n = 6), possum sp. (Trichosurus sp.; n = 8), and red foxes (Vulpes vulpes; n = 12) were analysed using piroplasm-specific 18S primers and Sanger sequencing. Seven Ixodes tasmani ticks from long-nosed bandicoots and bandicoots sp., three I. tasmani ticks and one Ixodes holocyclus tick from brushtail possums, and one Haemaphysalis longicornis tick from a red fox were positive for piroplasms. New genotypes, with sequences sharing 98% nucleotide similarities with Theileria sp. K1 detected in a burrowing bettong (Bettongia lesueur), were identified from bandicoot ticks. New genotypes were detected in ticks from brushtail possums, which shared 98% similarity with a Babesia sp. (JQ682877) previously identified in marsupials. Theileria orientalis was identified in the H. longicornis tick from the red fox. Babesia and Theileria spp. in the ticks parasitizing bandicoots and brushtail possums clustered closely with respective Babesia and Theileria clades derived from Australian marsupials. This represents the first detection of piroplasms in ticks ... Article in Journal/Newspaper Rattus rattus Directory of Open Access Journals: DOAJ Articles International Journal for Parasitology: Parasites and Wildlife 7 2 197 203 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Zoology QL1-991 |
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Zoology QL1-991 Siew-May Loh Siobhon Egan Amber Gillett Peter B. Banks Una M. Ryan Peter J. Irwin Charlotte L. Oskam Molecular surveillance of piroplasms in ticks from small and medium-sized urban and peri-urban mammals in Australia |
topic_facet |
Zoology QL1-991 |
description |
Natural landscape alterations as a consequence of urbanisation are one of the main drivers in the movements of wildlife into metropolitan and peri-urban areas. Worldwide, these wildlife species are highly adaptable and may be responsible for the transmission of tick-borne pathogens including piroplasms (Babesia, Theileria and Cytauxzoon spp.) that cause piroplasmosis in animals and occasionally in humans. Little is known about piroplasms in the ticks of urban wildlife in Australia. Ticks from long-nosed bandicoots (Perameles nasuta; n = 71), eastern-barred bandicoots (Perameles gunnii; n = 41), northern-brown bandicoots (Isoodon macrourus; n = 19), southern-brown bandicoots (Isoodon obesulus; n = 4), bandicoot sp. (n = 2), flying foxes (Pteropus sp.; n = 3), black rats (Rattus rattus; n = 7), bush rats (Rattus fuscipes; n = 4), brushtail possums (Trichosurus vulpecula; n = 19), ringtail possums (Pseudocheirus peregrinus; n = 12), short-eared possums (Trichosurus caninus; n = 6), possum sp. (Trichosurus sp.; n = 8), and red foxes (Vulpes vulpes; n = 12) were analysed using piroplasm-specific 18S primers and Sanger sequencing. Seven Ixodes tasmani ticks from long-nosed bandicoots and bandicoots sp., three I. tasmani ticks and one Ixodes holocyclus tick from brushtail possums, and one Haemaphysalis longicornis tick from a red fox were positive for piroplasms. New genotypes, with sequences sharing 98% nucleotide similarities with Theileria sp. K1 detected in a burrowing bettong (Bettongia lesueur), were identified from bandicoot ticks. New genotypes were detected in ticks from brushtail possums, which shared 98% similarity with a Babesia sp. (JQ682877) previously identified in marsupials. Theileria orientalis was identified in the H. longicornis tick from the red fox. Babesia and Theileria spp. in the ticks parasitizing bandicoots and brushtail possums clustered closely with respective Babesia and Theileria clades derived from Australian marsupials. This represents the first detection of piroplasms in ticks ... |
format |
Article in Journal/Newspaper |
author |
Siew-May Loh Siobhon Egan Amber Gillett Peter B. Banks Una M. Ryan Peter J. Irwin Charlotte L. Oskam |
author_facet |
Siew-May Loh Siobhon Egan Amber Gillett Peter B. Banks Una M. Ryan Peter J. Irwin Charlotte L. Oskam |
author_sort |
Siew-May Loh |
title |
Molecular surveillance of piroplasms in ticks from small and medium-sized urban and peri-urban mammals in Australia |
title_short |
Molecular surveillance of piroplasms in ticks from small and medium-sized urban and peri-urban mammals in Australia |
title_full |
Molecular surveillance of piroplasms in ticks from small and medium-sized urban and peri-urban mammals in Australia |
title_fullStr |
Molecular surveillance of piroplasms in ticks from small and medium-sized urban and peri-urban mammals in Australia |
title_full_unstemmed |
Molecular surveillance of piroplasms in ticks from small and medium-sized urban and peri-urban mammals in Australia |
title_sort |
molecular surveillance of piroplasms in ticks from small and medium-sized urban and peri-urban mammals in australia |
publisher |
Elsevier |
publishDate |
2018 |
url |
https://doi.org/10.1016/j.ijppaw.2018.05.005 https://doaj.org/article/a7c11bc51d124245a6390a742f21eac3 |
genre |
Rattus rattus |
genre_facet |
Rattus rattus |
op_source |
International Journal for Parasitology: Parasites and Wildlife, Vol 7, Iss 2, Pp 197-203 (2018) |
op_relation |
http://www.sciencedirect.com/science/article/pii/S2213224418300166 https://doaj.org/toc/2213-2244 2213-2244 doi:10.1016/j.ijppaw.2018.05.005 https://doaj.org/article/a7c11bc51d124245a6390a742f21eac3 |
op_doi |
https://doi.org/10.1016/j.ijppaw.2018.05.005 |
container_title |
International Journal for Parasitology: Parasites and Wildlife |
container_volume |
7 |
container_issue |
2 |
container_start_page |
197 |
op_container_end_page |
203 |
_version_ |
1766177138478678016 |