Therapeutic DNA vaccine encoding peptide P10 against experimental paracoccidioidomycosis.
Paracoccidioidomycosis (PCM), caused by Paracoccidioides brasiliensis, is the most prevalent invasive fungal disease in South America. Systemic mycoses are the 10th most common cause of death among infectious diseases in Brazil and PCM is responsible for more than 50% of deaths due to fungal infecti...
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ftdoajarticles:oai:doaj.org/article:a782b25d2a38448587e4a374f6dc4ffd 2023-05-15T15:11:02+02:00 Therapeutic DNA vaccine encoding peptide P10 against experimental paracoccidioidomycosis. Glauce M G Rittner Julián E Muñoz Alexandre F Marques Joshua D Nosanchuk Carlos P Taborda Luiz R Travassos 2012-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001519 https://doaj.org/article/a782b25d2a38448587e4a374f6dc4ffd EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3289603?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001519 https://doaj.org/article/a782b25d2a38448587e4a374f6dc4ffd PLoS Neglected Tropical Diseases, Vol 6, Iss 2, p e1519 (2012) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2012 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001519 2022-12-31T03:22:24Z Paracoccidioidomycosis (PCM), caused by Paracoccidioides brasiliensis, is the most prevalent invasive fungal disease in South America. Systemic mycoses are the 10th most common cause of death among infectious diseases in Brazil and PCM is responsible for more than 50% of deaths due to fungal infections. PCM is typically treated with sulfonamides, amphotericin B or azoles, although complete eradication of the fungus may not occur and relapsing disease is frequently reported. A 15-mer peptide from the major diagnostic antigen gp43, named P10, can induce a strong T-CD4+ helper-1 immune response in mice. The TEPITOPE algorithm and experimental data have confirmed that most HLA-DR molecules can present P10, which suggests that P10 is a candidate antigen for a PCM vaccine. In the current work, the therapeutic efficacy of plasmid immunization with P10 and/or IL-12 inserts was tested in murine models of PCM. When given prior to or after infection with P. brasiliensis virulent Pb 18 isolate, plasmid-vaccination with P10 and/or IL-12 inserts successfully reduced the fungal burden in lungs of infected mice. In fact, intramuscular administration of a combination of plasmids expressing P10 and IL-12 given weekly for one month, followed by single injections every month for 3 months restored normal lung architecture and eradicated the fungus in mice that were infected one month prior to treatment. The data indicate that immunization with these plasmids is a powerful procedure for prevention and treatment of experimental PCM, with the perspective of being also effective in human patients. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 6 2 e1519 |
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Directory of Open Access Journals: DOAJ Articles |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Glauce M G Rittner Julián E Muñoz Alexandre F Marques Joshua D Nosanchuk Carlos P Taborda Luiz R Travassos Therapeutic DNA vaccine encoding peptide P10 against experimental paracoccidioidomycosis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Paracoccidioidomycosis (PCM), caused by Paracoccidioides brasiliensis, is the most prevalent invasive fungal disease in South America. Systemic mycoses are the 10th most common cause of death among infectious diseases in Brazil and PCM is responsible for more than 50% of deaths due to fungal infections. PCM is typically treated with sulfonamides, amphotericin B or azoles, although complete eradication of the fungus may not occur and relapsing disease is frequently reported. A 15-mer peptide from the major diagnostic antigen gp43, named P10, can induce a strong T-CD4+ helper-1 immune response in mice. The TEPITOPE algorithm and experimental data have confirmed that most HLA-DR molecules can present P10, which suggests that P10 is a candidate antigen for a PCM vaccine. In the current work, the therapeutic efficacy of plasmid immunization with P10 and/or IL-12 inserts was tested in murine models of PCM. When given prior to or after infection with P. brasiliensis virulent Pb 18 isolate, plasmid-vaccination with P10 and/or IL-12 inserts successfully reduced the fungal burden in lungs of infected mice. In fact, intramuscular administration of a combination of plasmids expressing P10 and IL-12 given weekly for one month, followed by single injections every month for 3 months restored normal lung architecture and eradicated the fungus in mice that were infected one month prior to treatment. The data indicate that immunization with these plasmids is a powerful procedure for prevention and treatment of experimental PCM, with the perspective of being also effective in human patients. |
format |
Article in Journal/Newspaper |
author |
Glauce M G Rittner Julián E Muñoz Alexandre F Marques Joshua D Nosanchuk Carlos P Taborda Luiz R Travassos |
author_facet |
Glauce M G Rittner Julián E Muñoz Alexandre F Marques Joshua D Nosanchuk Carlos P Taborda Luiz R Travassos |
author_sort |
Glauce M G Rittner |
title |
Therapeutic DNA vaccine encoding peptide P10 against experimental paracoccidioidomycosis. |
title_short |
Therapeutic DNA vaccine encoding peptide P10 against experimental paracoccidioidomycosis. |
title_full |
Therapeutic DNA vaccine encoding peptide P10 against experimental paracoccidioidomycosis. |
title_fullStr |
Therapeutic DNA vaccine encoding peptide P10 against experimental paracoccidioidomycosis. |
title_full_unstemmed |
Therapeutic DNA vaccine encoding peptide P10 against experimental paracoccidioidomycosis. |
title_sort |
therapeutic dna vaccine encoding peptide p10 against experimental paracoccidioidomycosis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2012 |
url |
https://doi.org/10.1371/journal.pntd.0001519 https://doaj.org/article/a782b25d2a38448587e4a374f6dc4ffd |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 6, Iss 2, p e1519 (2012) |
op_relation |
http://europepmc.org/articles/PMC3289603?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001519 https://doaj.org/article/a782b25d2a38448587e4a374f6dc4ffd |
op_doi |
https://doi.org/10.1371/journal.pntd.0001519 |
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PLoS Neglected Tropical Diseases |
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6 |
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2 |
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e1519 |
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1766341947424768000 |