Evaluation of six commercial kits for the serological diagnosis of Mediterranean visceral leishmaniasis.
Background Zoonotic visceral leishmaniasis (VL) is endemic in the Mediterranean basin. However, large-scale comparative analyses of the commercial kits for the serological diagnosis of this neglected disease are lacking. This study compared the performances of four enzyme-linked immunosorbent assays...
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ftdoajarticles:oai:doaj.org/article:a7773021820e42a8962245fb943a0be6 2023-05-15T15:12:08+02:00 Evaluation of six commercial kits for the serological diagnosis of Mediterranean visceral leishmaniasis. Maude F Lévêque Emilie Battery Pascal Delaunay Badre Eddine Lmimouni Karim Aoun Coralie L'Ollivier Patrick Bastien Charles Mary Christelle Pomares Judith Fillaux Laurence Lachaud 2020-03-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0008139 https://doaj.org/article/a7773021820e42a8962245fb943a0be6 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0008139 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008139 https://doaj.org/article/a7773021820e42a8962245fb943a0be6 PLoS Neglected Tropical Diseases, Vol 14, Iss 3, p e0008139 (2020) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2020 ftdoajarticles https://doi.org/10.1371/journal.pntd.0008139 2022-12-31T11:44:55Z Background Zoonotic visceral leishmaniasis (VL) is endemic in the Mediterranean basin. However, large-scale comparative analyses of the commercial kits for the serological diagnosis of this neglected disease are lacking. This study compared the performances of four enzyme-linked immunosorbent assays (ELISA) and two immunochromatographic tests (ICT) as screening tests for the serodiagnosis of human VL in the Mediterranean region. Methodology/principal findings Serum samples from 319 patients living in France, Tunisia or Morocco were tested using two ICT (IT LEISH and TruQuick LEISH IgG/IgM Meridian) and four ELISA reagents (NovaLisa Leishmania infantum IgG, Bordier Leishmania infantum, Ridascreen Leishmania IgG, and Vircell Leishmania). The population with proven VL (n = 181) included 65 immunocompromised patients. Significantly higher percentages of false-negative results were obtained with all assays in immunocompromised patients, compared with the immunocompetent population. In the whole population, sensitivity and specificity ranged from 80.7% to 93.9% and from 95.7% to 100%, respectively. The maximum accuracy was observed with the Bordier and Vircell ELISA kits (96.2%), and the lowest accuracy with Ridascreen reagent (88.7%). New thresholds of positivity are proposed for the Bordier, Vircell and NovaLisa ELISA kits to achieve 95% sensitivity with the highest possible specificity. Western blot (WB), used as a confirmation method, showed 100% sensitivity and identified 10.1% of asymptomatic carriers among the control population from the South of France. Conclusions/significance This is the first study that compared commercially available kits for VL serodiagnosis in the endemic region of the Mediterranean basin. It provides specific information about the tests' performance to help clinicians and biologists to select the right assay for VL screening. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 14 3 e0008139 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Maude F Lévêque Emilie Battery Pascal Delaunay Badre Eddine Lmimouni Karim Aoun Coralie L'Ollivier Patrick Bastien Charles Mary Christelle Pomares Judith Fillaux Laurence Lachaud Evaluation of six commercial kits for the serological diagnosis of Mediterranean visceral leishmaniasis. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Background Zoonotic visceral leishmaniasis (VL) is endemic in the Mediterranean basin. However, large-scale comparative analyses of the commercial kits for the serological diagnosis of this neglected disease are lacking. This study compared the performances of four enzyme-linked immunosorbent assays (ELISA) and two immunochromatographic tests (ICT) as screening tests for the serodiagnosis of human VL in the Mediterranean region. Methodology/principal findings Serum samples from 319 patients living in France, Tunisia or Morocco were tested using two ICT (IT LEISH and TruQuick LEISH IgG/IgM Meridian) and four ELISA reagents (NovaLisa Leishmania infantum IgG, Bordier Leishmania infantum, Ridascreen Leishmania IgG, and Vircell Leishmania). The population with proven VL (n = 181) included 65 immunocompromised patients. Significantly higher percentages of false-negative results were obtained with all assays in immunocompromised patients, compared with the immunocompetent population. In the whole population, sensitivity and specificity ranged from 80.7% to 93.9% and from 95.7% to 100%, respectively. The maximum accuracy was observed with the Bordier and Vircell ELISA kits (96.2%), and the lowest accuracy with Ridascreen reagent (88.7%). New thresholds of positivity are proposed for the Bordier, Vircell and NovaLisa ELISA kits to achieve 95% sensitivity with the highest possible specificity. Western blot (WB), used as a confirmation method, showed 100% sensitivity and identified 10.1% of asymptomatic carriers among the control population from the South of France. Conclusions/significance This is the first study that compared commercially available kits for VL serodiagnosis in the endemic region of the Mediterranean basin. It provides specific information about the tests' performance to help clinicians and biologists to select the right assay for VL screening. |
format |
Article in Journal/Newspaper |
author |
Maude F Lévêque Emilie Battery Pascal Delaunay Badre Eddine Lmimouni Karim Aoun Coralie L'Ollivier Patrick Bastien Charles Mary Christelle Pomares Judith Fillaux Laurence Lachaud |
author_facet |
Maude F Lévêque Emilie Battery Pascal Delaunay Badre Eddine Lmimouni Karim Aoun Coralie L'Ollivier Patrick Bastien Charles Mary Christelle Pomares Judith Fillaux Laurence Lachaud |
author_sort |
Maude F Lévêque |
title |
Evaluation of six commercial kits for the serological diagnosis of Mediterranean visceral leishmaniasis. |
title_short |
Evaluation of six commercial kits for the serological diagnosis of Mediterranean visceral leishmaniasis. |
title_full |
Evaluation of six commercial kits for the serological diagnosis of Mediterranean visceral leishmaniasis. |
title_fullStr |
Evaluation of six commercial kits for the serological diagnosis of Mediterranean visceral leishmaniasis. |
title_full_unstemmed |
Evaluation of six commercial kits for the serological diagnosis of Mediterranean visceral leishmaniasis. |
title_sort |
evaluation of six commercial kits for the serological diagnosis of mediterranean visceral leishmaniasis. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2020 |
url |
https://doi.org/10.1371/journal.pntd.0008139 https://doaj.org/article/a7773021820e42a8962245fb943a0be6 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 14, Iss 3, p e0008139 (2020) |
op_relation |
https://doi.org/10.1371/journal.pntd.0008139 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0008139 https://doaj.org/article/a7773021820e42a8962245fb943a0be6 |
op_doi |
https://doi.org/10.1371/journal.pntd.0008139 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
14 |
container_issue |
3 |
container_start_page |
e0008139 |
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1766342864198959104 |