Technical viability of the YF MAC-HD ELISA kit for use in yellow fever-endemic regions.
Yellow fever (YF), an arboviral disease, affects an estimated 200,000 people and causes 30,000 deaths per year and recently has caused major epidemics in Africa and South America. Timely and accurate diagnosis of YF is critical for managing outbreaks and implementing vaccination campaigns. A YF immu...
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ftdoajarticles:oai:doaj.org/article:a73cadbd193c46158aee4c7fb0f13253 2023-05-15T15:07:12+02:00 Technical viability of the YF MAC-HD ELISA kit for use in yellow fever-endemic regions. Christin H Goodman Maurice Demanou Mick Mulders Jairo Mendez-Rico Alison Jane Basile 2021-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0009417 https://doaj.org/article/a73cadbd193c46158aee4c7fb0f13253 EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0009417 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009417 https://doaj.org/article/a73cadbd193c46158aee4c7fb0f13253 PLoS Neglected Tropical Diseases, Vol 15, Iss 6, p e0009417 (2021) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2021 ftdoajarticles https://doi.org/10.1371/journal.pntd.0009417 2022-12-31T09:16:08Z Yellow fever (YF), an arboviral disease, affects an estimated 200,000 people and causes 30,000 deaths per year and recently has caused major epidemics in Africa and South America. Timely and accurate diagnosis of YF is critical for managing outbreaks and implementing vaccination campaigns. A YF immunoglobulin M (IgM) antibody-capture (MAC) enzyme-linked immunosorbent assay (ELISA) kit, the YF MAC-HD, was successfully introduced starting in 2018 to laboratories in Africa and South America. The YF MAC-HD kit can be performed in 3.5 hours, test up to 24 samples, and includes all reagents necessary to perform the test, except for water used to dilute wash buffer. In 2018 and 2019, a total of 56 laboratory personnel from 39 countries in Africa and South America were trained to use the kit during workshops, followed by take-home YF IgM proficiency testing (PT) exercises. Participants received either a 10- or 20-sample YF PT panel and performed testing using the YF MAC-HD kit. All countries obtained 90% or higher correct results. These results verified the technical viability and transferability of YF MAC-HD kit use for laboratories in YF-endemic countries. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 15 6 e0009417 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Christin H Goodman Maurice Demanou Mick Mulders Jairo Mendez-Rico Alison Jane Basile Technical viability of the YF MAC-HD ELISA kit for use in yellow fever-endemic regions. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Yellow fever (YF), an arboviral disease, affects an estimated 200,000 people and causes 30,000 deaths per year and recently has caused major epidemics in Africa and South America. Timely and accurate diagnosis of YF is critical for managing outbreaks and implementing vaccination campaigns. A YF immunoglobulin M (IgM) antibody-capture (MAC) enzyme-linked immunosorbent assay (ELISA) kit, the YF MAC-HD, was successfully introduced starting in 2018 to laboratories in Africa and South America. The YF MAC-HD kit can be performed in 3.5 hours, test up to 24 samples, and includes all reagents necessary to perform the test, except for water used to dilute wash buffer. In 2018 and 2019, a total of 56 laboratory personnel from 39 countries in Africa and South America were trained to use the kit during workshops, followed by take-home YF IgM proficiency testing (PT) exercises. Participants received either a 10- or 20-sample YF PT panel and performed testing using the YF MAC-HD kit. All countries obtained 90% or higher correct results. These results verified the technical viability and transferability of YF MAC-HD kit use for laboratories in YF-endemic countries. |
format |
Article in Journal/Newspaper |
author |
Christin H Goodman Maurice Demanou Mick Mulders Jairo Mendez-Rico Alison Jane Basile |
author_facet |
Christin H Goodman Maurice Demanou Mick Mulders Jairo Mendez-Rico Alison Jane Basile |
author_sort |
Christin H Goodman |
title |
Technical viability of the YF MAC-HD ELISA kit for use in yellow fever-endemic regions. |
title_short |
Technical viability of the YF MAC-HD ELISA kit for use in yellow fever-endemic regions. |
title_full |
Technical viability of the YF MAC-HD ELISA kit for use in yellow fever-endemic regions. |
title_fullStr |
Technical viability of the YF MAC-HD ELISA kit for use in yellow fever-endemic regions. |
title_full_unstemmed |
Technical viability of the YF MAC-HD ELISA kit for use in yellow fever-endemic regions. |
title_sort |
technical viability of the yf mac-hd elisa kit for use in yellow fever-endemic regions. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2021 |
url |
https://doi.org/10.1371/journal.pntd.0009417 https://doaj.org/article/a73cadbd193c46158aee4c7fb0f13253 |
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Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 15, Iss 6, p e0009417 (2021) |
op_relation |
https://doi.org/10.1371/journal.pntd.0009417 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0009417 https://doaj.org/article/a73cadbd193c46158aee4c7fb0f13253 |
op_doi |
https://doi.org/10.1371/journal.pntd.0009417 |
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PLOS Neglected Tropical Diseases |
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15 |
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6 |
container_start_page |
e0009417 |
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