Infection of the malaria vector Anopheles coluzzii with the entomopathogenic bacteria Chromobacterium anophelis sp. nov. IRSSSOUMB001 reduces larval survival and adult reproductive potential
Abstract Background Vector control tools are urgently needed to control malaria transmission in Africa. A native strain of Chromobacterium sp. from Burkina Faso was recently isolated and preliminarily named Chromobacterium anophelis sp. nov. IRSSSOUMB001. In bioassays, this bacterium showed a promis...
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ftdoajarticles:oai:doaj.org/article:a722020e404b430bb31efaca6b2bfdc8 2023-06-11T04:09:54+02:00 Infection of the malaria vector Anopheles coluzzii with the entomopathogenic bacteria Chromobacterium anophelis sp. nov. IRSSSOUMB001 reduces larval survival and adult reproductive potential Edounou Jacques Gnambani Etienne Bilgo Roch K. Dabiré Adrien Marie Gaston Belem Abdoulaye Diabaté 2023-04-01T00:00:00Z https://doi.org/10.1186/s12936-023-04551-0 https://doaj.org/article/a722020e404b430bb31efaca6b2bfdc8 EN eng BMC https://doi.org/10.1186/s12936-023-04551-0 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-023-04551-0 1475-2875 https://doaj.org/article/a722020e404b430bb31efaca6b2bfdc8 Malaria Journal, Vol 22, Iss 1, Pp 1-7 (2023) Chromobacterium anophelis sp. nov IRSSSOUMB001 Mosquito larvae Insemination rate Wings Anopheles coluzzii Malaria Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2023 ftdoajarticles https://doi.org/10.1186/s12936-023-04551-0 2023-04-23T00:36:47Z Abstract Background Vector control tools are urgently needed to control malaria transmission in Africa. A native strain of Chromobacterium sp. from Burkina Faso was recently isolated and preliminarily named Chromobacterium anophelis sp. nov. IRSSSOUMB001. In bioassays, this bacterium showed a promising virulence against adult mosquitoes and reduces their blood feeding propensity and fecundity. The current study assessed the entomopathogenic effects of C. anophelis IRSSSOUMB001 on larval stages of mosquitoes, as well as its impacts on infected mosquitoes reproductive capacity and trans-generational effects. Methods Virulence on larvae and interference with insemination were assayed by co-incubation with C. anophelis IRSSSOUMB001 at a range of 104 to 108 cfu/ml. Trans-generational effects were determined by measuring body size differences of progeny from infected vs. uninfected parent mosquitoes using wing size as a proxy. Results Chromobacterium anophelis IRSSSOUMB001 killed larvae of the pyrethroid-resistant Anopheles coluzzii with LT80 of ~ 1.75 ± 0.14 days at 108 cfu/ml in larval breeding trays. Reproductive success was reduced as a measure of insemination rate from 95 ± 1.99% to 21 ± 3.76% for the infected females. There was a difference in wing sizes between control and infected mosquito offsprings from 2.55 ± 0.17 mm to 2.1 ± 0.21 mm in infected females, and from 2.43 ± 0.13 mm to 1.99 ± 0.15 mm in infected males. Conclusions This study showed that C. anophelis IRSSSOUMB001 was highly virulent against larvae of insecticide-resistant Anopheles coluzzii, and reduced both mosquito reproduction capacity and offspring fitness. Additional laboratory, field, safety and social acceptance studies are needed to draw firm conclusions about the practical utility of this bacterial strain for malaria vector control. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Malaria Journal 22 1 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
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Chromobacterium anophelis sp. nov IRSSSOUMB001 Mosquito larvae Insemination rate Wings Anopheles coluzzii Malaria Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
spellingShingle |
Chromobacterium anophelis sp. nov IRSSSOUMB001 Mosquito larvae Insemination rate Wings Anopheles coluzzii Malaria Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Edounou Jacques Gnambani Etienne Bilgo Roch K. Dabiré Adrien Marie Gaston Belem Abdoulaye Diabaté Infection of the malaria vector Anopheles coluzzii with the entomopathogenic bacteria Chromobacterium anophelis sp. nov. IRSSSOUMB001 reduces larval survival and adult reproductive potential |
topic_facet |
Chromobacterium anophelis sp. nov IRSSSOUMB001 Mosquito larvae Insemination rate Wings Anopheles coluzzii Malaria Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Vector control tools are urgently needed to control malaria transmission in Africa. A native strain of Chromobacterium sp. from Burkina Faso was recently isolated and preliminarily named Chromobacterium anophelis sp. nov. IRSSSOUMB001. In bioassays, this bacterium showed a promising virulence against adult mosquitoes and reduces their blood feeding propensity and fecundity. The current study assessed the entomopathogenic effects of C. anophelis IRSSSOUMB001 on larval stages of mosquitoes, as well as its impacts on infected mosquitoes reproductive capacity and trans-generational effects. Methods Virulence on larvae and interference with insemination were assayed by co-incubation with C. anophelis IRSSSOUMB001 at a range of 104 to 108 cfu/ml. Trans-generational effects were determined by measuring body size differences of progeny from infected vs. uninfected parent mosquitoes using wing size as a proxy. Results Chromobacterium anophelis IRSSSOUMB001 killed larvae of the pyrethroid-resistant Anopheles coluzzii with LT80 of ~ 1.75 ± 0.14 days at 108 cfu/ml in larval breeding trays. Reproductive success was reduced as a measure of insemination rate from 95 ± 1.99% to 21 ± 3.76% for the infected females. There was a difference in wing sizes between control and infected mosquito offsprings from 2.55 ± 0.17 mm to 2.1 ± 0.21 mm in infected females, and from 2.43 ± 0.13 mm to 1.99 ± 0.15 mm in infected males. Conclusions This study showed that C. anophelis IRSSSOUMB001 was highly virulent against larvae of insecticide-resistant Anopheles coluzzii, and reduced both mosquito reproduction capacity and offspring fitness. Additional laboratory, field, safety and social acceptance studies are needed to draw firm conclusions about the practical utility of this bacterial strain for malaria vector control. |
format |
Article in Journal/Newspaper |
author |
Edounou Jacques Gnambani Etienne Bilgo Roch K. Dabiré Adrien Marie Gaston Belem Abdoulaye Diabaté |
author_facet |
Edounou Jacques Gnambani Etienne Bilgo Roch K. Dabiré Adrien Marie Gaston Belem Abdoulaye Diabaté |
author_sort |
Edounou Jacques Gnambani |
title |
Infection of the malaria vector Anopheles coluzzii with the entomopathogenic bacteria Chromobacterium anophelis sp. nov. IRSSSOUMB001 reduces larval survival and adult reproductive potential |
title_short |
Infection of the malaria vector Anopheles coluzzii with the entomopathogenic bacteria Chromobacterium anophelis sp. nov. IRSSSOUMB001 reduces larval survival and adult reproductive potential |
title_full |
Infection of the malaria vector Anopheles coluzzii with the entomopathogenic bacteria Chromobacterium anophelis sp. nov. IRSSSOUMB001 reduces larval survival and adult reproductive potential |
title_fullStr |
Infection of the malaria vector Anopheles coluzzii with the entomopathogenic bacteria Chromobacterium anophelis sp. nov. IRSSSOUMB001 reduces larval survival and adult reproductive potential |
title_full_unstemmed |
Infection of the malaria vector Anopheles coluzzii with the entomopathogenic bacteria Chromobacterium anophelis sp. nov. IRSSSOUMB001 reduces larval survival and adult reproductive potential |
title_sort |
infection of the malaria vector anopheles coluzzii with the entomopathogenic bacteria chromobacterium anophelis sp. nov. irsssoumb001 reduces larval survival and adult reproductive potential |
publisher |
BMC |
publishDate |
2023 |
url |
https://doi.org/10.1186/s12936-023-04551-0 https://doaj.org/article/a722020e404b430bb31efaca6b2bfdc8 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 22, Iss 1, Pp 1-7 (2023) |
op_relation |
https://doi.org/10.1186/s12936-023-04551-0 https://doaj.org/toc/1475-2875 doi:10.1186/s12936-023-04551-0 1475-2875 https://doaj.org/article/a722020e404b430bb31efaca6b2bfdc8 |
op_doi |
https://doi.org/10.1186/s12936-023-04551-0 |
container_title |
Malaria Journal |
container_volume |
22 |
container_issue |
1 |
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1768383918650687488 |