Detection of 1014F kdr mutation in four major Anopheline malaria vectors in Indonesia
Abstract Background Malaria is a serious public health problem in Indonesia, particularly in areas outside Java and Bali. The spread of resistance to the currently available anti-malarial drugs or insecticides used for mosquito control would cause an increase in malaria transmission. To better under...
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ftdoajarticles:oai:doaj.org/article:a71c8531fa4849e0b6845ff6057fb0d8 2023-05-15T15:10:00+02:00 Detection of 1014F kdr mutation in four major Anopheline malaria vectors in Indonesia Sukowati Supratman Hawley William A Asih Puji BS Hidayati Anggi PN Syafruddin Din Lobo Neil F 2010-11-01T00:00:00Z https://doi.org/10.1186/1475-2875-9-315 https://doaj.org/article/a71c8531fa4849e0b6845ff6057fb0d8 EN eng BMC http://www.malariajournal.com/content/9/1/315 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-9-315 1475-2875 https://doaj.org/article/a71c8531fa4849e0b6845ff6057fb0d8 Malaria Journal, Vol 9, Iss 1, p 315 (2010) Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 article 2010 ftdoajarticles https://doi.org/10.1186/1475-2875-9-315 2022-12-31T08:50:52Z Abstract Background Malaria is a serious public health problem in Indonesia, particularly in areas outside Java and Bali. The spread of resistance to the currently available anti-malarial drugs or insecticides used for mosquito control would cause an increase in malaria transmission. To better understand patterns of transmission and resistance in Indonesia, an integrated mosquito survey was conducted in three areas with different malaria endemicities, Purworejo in Central Java, South Lampung District in Sumatera and South Halmahera District in North Mollucca. Methods Mosquitoes were collected from the three areas through indoor and outdoor human landing catches (HLC) and indoor restinging catches. Specimens were identified morphologically by species and kept individually in 1.5 ml Eppendorf microtube. A fragment of the VGSC gene from 95 mosquito samples was sequenced and kdr allelic variation determined. Results The molecular analysis of these anopheline mosquitoes revealed the existence of the 1014F allele in 4 major malaria vectors from South Lampung. These species include, Anopheles sundaicus, Anopheles aconitus, Anopheles subpictus and Anopheles vagus . The 1014F allele was not found in the other areas. Conclusion The finding documents the presence of this mutant allele in Indonesia, and implies that selection pressure on the Anopheles population in this area has occurred. Further studies to determine the impact of the resistance allele on the efficacy of pyrethroids in control programmes are needed. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Bali ENVELOPE(-20.233,-20.233,64.067,64.067) Malaria Journal 9 1 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
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Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 Sukowati Supratman Hawley William A Asih Puji BS Hidayati Anggi PN Syafruddin Din Lobo Neil F Detection of 1014F kdr mutation in four major Anopheline malaria vectors in Indonesia |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Infectious and parasitic diseases RC109-216 |
description |
Abstract Background Malaria is a serious public health problem in Indonesia, particularly in areas outside Java and Bali. The spread of resistance to the currently available anti-malarial drugs or insecticides used for mosquito control would cause an increase in malaria transmission. To better understand patterns of transmission and resistance in Indonesia, an integrated mosquito survey was conducted in three areas with different malaria endemicities, Purworejo in Central Java, South Lampung District in Sumatera and South Halmahera District in North Mollucca. Methods Mosquitoes were collected from the three areas through indoor and outdoor human landing catches (HLC) and indoor restinging catches. Specimens were identified morphologically by species and kept individually in 1.5 ml Eppendorf microtube. A fragment of the VGSC gene from 95 mosquito samples was sequenced and kdr allelic variation determined. Results The molecular analysis of these anopheline mosquitoes revealed the existence of the 1014F allele in 4 major malaria vectors from South Lampung. These species include, Anopheles sundaicus, Anopheles aconitus, Anopheles subpictus and Anopheles vagus . The 1014F allele was not found in the other areas. Conclusion The finding documents the presence of this mutant allele in Indonesia, and implies that selection pressure on the Anopheles population in this area has occurred. Further studies to determine the impact of the resistance allele on the efficacy of pyrethroids in control programmes are needed. |
format |
Article in Journal/Newspaper |
author |
Sukowati Supratman Hawley William A Asih Puji BS Hidayati Anggi PN Syafruddin Din Lobo Neil F |
author_facet |
Sukowati Supratman Hawley William A Asih Puji BS Hidayati Anggi PN Syafruddin Din Lobo Neil F |
author_sort |
Sukowati Supratman |
title |
Detection of 1014F kdr mutation in four major Anopheline malaria vectors in Indonesia |
title_short |
Detection of 1014F kdr mutation in four major Anopheline malaria vectors in Indonesia |
title_full |
Detection of 1014F kdr mutation in four major Anopheline malaria vectors in Indonesia |
title_fullStr |
Detection of 1014F kdr mutation in four major Anopheline malaria vectors in Indonesia |
title_full_unstemmed |
Detection of 1014F kdr mutation in four major Anopheline malaria vectors in Indonesia |
title_sort |
detection of 1014f kdr mutation in four major anopheline malaria vectors in indonesia |
publisher |
BMC |
publishDate |
2010 |
url |
https://doi.org/10.1186/1475-2875-9-315 https://doaj.org/article/a71c8531fa4849e0b6845ff6057fb0d8 |
long_lat |
ENVELOPE(-20.233,-20.233,64.067,64.067) |
geographic |
Arctic Bali |
geographic_facet |
Arctic Bali |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Malaria Journal, Vol 9, Iss 1, p 315 (2010) |
op_relation |
http://www.malariajournal.com/content/9/1/315 https://doaj.org/toc/1475-2875 doi:10.1186/1475-2875-9-315 1475-2875 https://doaj.org/article/a71c8531fa4849e0b6845ff6057fb0d8 |
op_doi |
https://doi.org/10.1186/1475-2875-9-315 |
container_title |
Malaria Journal |
container_volume |
9 |
container_issue |
1 |
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1766341077421260800 |