Bioactivity-Guided Screening of Antimicrobial Secondary Metabolites from Antarctic Cultivable Fungus Acrostalagmus luteoalbus CH-6 Combined with Molecular Networking
With the increasingly serious antimicrobial resistance, discovering novel antibiotics has grown impendency. The Antarctic abundant microbial resources, especially fungi, can produce unique bioactive compounds for adapting to the hostile environment. In this study, three Antarctic fungi, Chrysosporiu...
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ftdoajarticles:oai:doaj.org/article:a70676f9346743a2b53cb26ffbd7c367 2023-05-15T13:38:09+02:00 Bioactivity-Guided Screening of Antimicrobial Secondary Metabolites from Antarctic Cultivable Fungus Acrostalagmus luteoalbus CH-6 Combined with Molecular Networking Ting Shi Xiang-Qian Li Ze-Min Wang Li Zheng Yan-Yan Yu Jia-Jia Dai Da-Yong Shi 2022-05-01T00:00:00Z https://doi.org/10.3390/md20050334 https://doaj.org/article/a70676f9346743a2b53cb26ffbd7c367 EN eng MDPI AG https://www.mdpi.com/1660-3397/20/5/334 https://doaj.org/toc/1660-3397 doi:10.3390/md20050334 1660-3397 https://doaj.org/article/a70676f9346743a2b53cb26ffbd7c367 Marine Drugs, Vol 20, Iss 334, p 334 (2022) Antarctic fungi bioactivity-guided screening molecular networking antimicrobial activities secondary metabolites Acrostalagmus luteoalbus Biology (General) QH301-705.5 article 2022 ftdoajarticles https://doi.org/10.3390/md20050334 2022-12-30T22:33:36Z With the increasingly serious antimicrobial resistance, discovering novel antibiotics has grown impendency. The Antarctic abundant microbial resources, especially fungi, can produce unique bioactive compounds for adapting to the hostile environment. In this study, three Antarctic fungi, Chrysosporium sp. HSXSD-11-1, Cladosporium sp. HSXSD-12 and Acrostalagmus luteoalbus CH-6, were found to have the potential to produce antimicrobial compounds. Furthermore, the crude extracts of CH-6 displayed the strongest antimicrobial activities with 72.3–84.8% growth inhibition against C. albicans and Aeromonas salmonicida . The secondary metabolites of CH-6 were researched by bioactivity tracking combined with molecular networking and led to the isolation of two new α -pyrones, acrostalapyrones A ( 1 ) and B ( 2 ), along with one known analog ( 3 ), and three known indole diketopiperazines ( 4 – 6 ). The absolute configurations of 1 and 2 were identified through modified Mosher’s method. Compounds 4 and 6 showed strong antimicrobial activities. Remarkably, the antibacterial activity of 6 against A. salmonicida displayed two times higher than that of the positive drug Ciprofloxacin. This is the first report to discover α -pyrones from the genus Acrostalagmus , and the significant antimicrobial activities of 4 and 6 against C. albicans and A. salmonicida . This study further demonstrates the great potential of Antarctic fungi in the development of new compounds and antibiotics. Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic Marine Drugs 20 5 334 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Antarctic fungi bioactivity-guided screening molecular networking antimicrobial activities secondary metabolites Acrostalagmus luteoalbus Biology (General) QH301-705.5 |
spellingShingle |
Antarctic fungi bioactivity-guided screening molecular networking antimicrobial activities secondary metabolites Acrostalagmus luteoalbus Biology (General) QH301-705.5 Ting Shi Xiang-Qian Li Ze-Min Wang Li Zheng Yan-Yan Yu Jia-Jia Dai Da-Yong Shi Bioactivity-Guided Screening of Antimicrobial Secondary Metabolites from Antarctic Cultivable Fungus Acrostalagmus luteoalbus CH-6 Combined with Molecular Networking |
topic_facet |
Antarctic fungi bioactivity-guided screening molecular networking antimicrobial activities secondary metabolites Acrostalagmus luteoalbus Biology (General) QH301-705.5 |
description |
With the increasingly serious antimicrobial resistance, discovering novel antibiotics has grown impendency. The Antarctic abundant microbial resources, especially fungi, can produce unique bioactive compounds for adapting to the hostile environment. In this study, three Antarctic fungi, Chrysosporium sp. HSXSD-11-1, Cladosporium sp. HSXSD-12 and Acrostalagmus luteoalbus CH-6, were found to have the potential to produce antimicrobial compounds. Furthermore, the crude extracts of CH-6 displayed the strongest antimicrobial activities with 72.3–84.8% growth inhibition against C. albicans and Aeromonas salmonicida . The secondary metabolites of CH-6 were researched by bioactivity tracking combined with molecular networking and led to the isolation of two new α -pyrones, acrostalapyrones A ( 1 ) and B ( 2 ), along with one known analog ( 3 ), and three known indole diketopiperazines ( 4 – 6 ). The absolute configurations of 1 and 2 were identified through modified Mosher’s method. Compounds 4 and 6 showed strong antimicrobial activities. Remarkably, the antibacterial activity of 6 against A. salmonicida displayed two times higher than that of the positive drug Ciprofloxacin. This is the first report to discover α -pyrones from the genus Acrostalagmus , and the significant antimicrobial activities of 4 and 6 against C. albicans and A. salmonicida . This study further demonstrates the great potential of Antarctic fungi in the development of new compounds and antibiotics. |
format |
Article in Journal/Newspaper |
author |
Ting Shi Xiang-Qian Li Ze-Min Wang Li Zheng Yan-Yan Yu Jia-Jia Dai Da-Yong Shi |
author_facet |
Ting Shi Xiang-Qian Li Ze-Min Wang Li Zheng Yan-Yan Yu Jia-Jia Dai Da-Yong Shi |
author_sort |
Ting Shi |
title |
Bioactivity-Guided Screening of Antimicrobial Secondary Metabolites from Antarctic Cultivable Fungus Acrostalagmus luteoalbus CH-6 Combined with Molecular Networking |
title_short |
Bioactivity-Guided Screening of Antimicrobial Secondary Metabolites from Antarctic Cultivable Fungus Acrostalagmus luteoalbus CH-6 Combined with Molecular Networking |
title_full |
Bioactivity-Guided Screening of Antimicrobial Secondary Metabolites from Antarctic Cultivable Fungus Acrostalagmus luteoalbus CH-6 Combined with Molecular Networking |
title_fullStr |
Bioactivity-Guided Screening of Antimicrobial Secondary Metabolites from Antarctic Cultivable Fungus Acrostalagmus luteoalbus CH-6 Combined with Molecular Networking |
title_full_unstemmed |
Bioactivity-Guided Screening of Antimicrobial Secondary Metabolites from Antarctic Cultivable Fungus Acrostalagmus luteoalbus CH-6 Combined with Molecular Networking |
title_sort |
bioactivity-guided screening of antimicrobial secondary metabolites from antarctic cultivable fungus acrostalagmus luteoalbus ch-6 combined with molecular networking |
publisher |
MDPI AG |
publishDate |
2022 |
url |
https://doi.org/10.3390/md20050334 https://doaj.org/article/a70676f9346743a2b53cb26ffbd7c367 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
Marine Drugs, Vol 20, Iss 334, p 334 (2022) |
op_relation |
https://www.mdpi.com/1660-3397/20/5/334 https://doaj.org/toc/1660-3397 doi:10.3390/md20050334 1660-3397 https://doaj.org/article/a70676f9346743a2b53cb26ffbd7c367 |
op_doi |
https://doi.org/10.3390/md20050334 |
container_title |
Marine Drugs |
container_volume |
20 |
container_issue |
5 |
container_start_page |
334 |
_version_ |
1766101977353158656 |