Outer-inner membrane vesicles naturally secreted by gram-negative pathogenic bacteria.
Outer-inner membrane vesicles (O-IMVs) were recently described as a new type of membrane vesicle secreted by the Antarctic bacterium Shewanella vesiculosa M7T. Their formation is characterized by the protrusion of both outer and plasma membranes, which pulls cytoplasmic components into the vesicles....
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ftdoajarticles:oai:doaj.org/article:a6ec39363ffe41beb71a1db212030522 2023-05-15T14:02:02+02:00 Outer-inner membrane vesicles naturally secreted by gram-negative pathogenic bacteria. Carla Pérez-Cruz Lidia Delgado Carmen López-Iglesias Elena Mercade 2015-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0116896 https://doaj.org/article/a6ec39363ffe41beb71a1db212030522 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4291224?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0116896 https://doaj.org/article/a6ec39363ffe41beb71a1db212030522 PLoS ONE, Vol 10, Iss 1, p e0116896 (2015) Medicine R Science Q article 2015 ftdoajarticles https://doi.org/10.1371/journal.pone.0116896 2022-12-31T00:13:07Z Outer-inner membrane vesicles (O-IMVs) were recently described as a new type of membrane vesicle secreted by the Antarctic bacterium Shewanella vesiculosa M7T. Their formation is characterized by the protrusion of both outer and plasma membranes, which pulls cytoplasmic components into the vesicles. To demonstrate that this is not a singular phenomenon in a bacterium occurring in an extreme environment, the identification of O-IMVs in pathogenic bacteria was undertaken. With this aim, a structural study by Transmission Electron Microscopy (TEM) and Cryo-transmission electron microscopy (Cryo-TEM) was carried out, confirming that O-IMVs are also secreted by Gram-negative pathogenic bacteria such as Neisseria gonorrhoeae, Pseudomonas aeruginosa PAO1 and Acinetobacter baumannii AB41, in which they represent between 0.23% and 1.2% of total vesicles produced. DNA and ATP, which are components solely found in the cell cytoplasm, were identified within membrane vesicles of these strains. The presence of DNA inside the O-IMVs produced by N. gonorrhoeae was confirmed by gold DNA immunolabeling with a specific monoclonal IgM against double-stranded DNA. A proteomic analysis of N. gonorrhoeae-derived membrane vesicles identified proteins from the cytoplasm and plasma membrane. This confirmation of O-IMV extends the hitherto uniform definition of membrane vesicles in Gram-negative bacteria and explains the presence of components in membrane vesicles such as DNA, cytoplasmic and inner membrane proteins, as well as ATP, detected for the first time. The production of these O-IMVs by pathogenic Gram-negative bacteria opens up new areas of study related to their involvement in lateral gene transfer, the transfer of cytoplasmic proteins, as well as the functionality and role of ATP detected in these new vesicles. Article in Journal/Newspaper Antarc* Antarctic Directory of Open Access Journals: DOAJ Articles Antarctic The Antarctic PLOS ONE 10 1 e0116896 |
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Directory of Open Access Journals: DOAJ Articles |
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Medicine R Science Q |
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Medicine R Science Q Carla Pérez-Cruz Lidia Delgado Carmen López-Iglesias Elena Mercade Outer-inner membrane vesicles naturally secreted by gram-negative pathogenic bacteria. |
topic_facet |
Medicine R Science Q |
description |
Outer-inner membrane vesicles (O-IMVs) were recently described as a new type of membrane vesicle secreted by the Antarctic bacterium Shewanella vesiculosa M7T. Their formation is characterized by the protrusion of both outer and plasma membranes, which pulls cytoplasmic components into the vesicles. To demonstrate that this is not a singular phenomenon in a bacterium occurring in an extreme environment, the identification of O-IMVs in pathogenic bacteria was undertaken. With this aim, a structural study by Transmission Electron Microscopy (TEM) and Cryo-transmission electron microscopy (Cryo-TEM) was carried out, confirming that O-IMVs are also secreted by Gram-negative pathogenic bacteria such as Neisseria gonorrhoeae, Pseudomonas aeruginosa PAO1 and Acinetobacter baumannii AB41, in which they represent between 0.23% and 1.2% of total vesicles produced. DNA and ATP, which are components solely found in the cell cytoplasm, were identified within membrane vesicles of these strains. The presence of DNA inside the O-IMVs produced by N. gonorrhoeae was confirmed by gold DNA immunolabeling with a specific monoclonal IgM against double-stranded DNA. A proteomic analysis of N. gonorrhoeae-derived membrane vesicles identified proteins from the cytoplasm and plasma membrane. This confirmation of O-IMV extends the hitherto uniform definition of membrane vesicles in Gram-negative bacteria and explains the presence of components in membrane vesicles such as DNA, cytoplasmic and inner membrane proteins, as well as ATP, detected for the first time. The production of these O-IMVs by pathogenic Gram-negative bacteria opens up new areas of study related to their involvement in lateral gene transfer, the transfer of cytoplasmic proteins, as well as the functionality and role of ATP detected in these new vesicles. |
format |
Article in Journal/Newspaper |
author |
Carla Pérez-Cruz Lidia Delgado Carmen López-Iglesias Elena Mercade |
author_facet |
Carla Pérez-Cruz Lidia Delgado Carmen López-Iglesias Elena Mercade |
author_sort |
Carla Pérez-Cruz |
title |
Outer-inner membrane vesicles naturally secreted by gram-negative pathogenic bacteria. |
title_short |
Outer-inner membrane vesicles naturally secreted by gram-negative pathogenic bacteria. |
title_full |
Outer-inner membrane vesicles naturally secreted by gram-negative pathogenic bacteria. |
title_fullStr |
Outer-inner membrane vesicles naturally secreted by gram-negative pathogenic bacteria. |
title_full_unstemmed |
Outer-inner membrane vesicles naturally secreted by gram-negative pathogenic bacteria. |
title_sort |
outer-inner membrane vesicles naturally secreted by gram-negative pathogenic bacteria. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pone.0116896 https://doaj.org/article/a6ec39363ffe41beb71a1db212030522 |
geographic |
Antarctic The Antarctic |
geographic_facet |
Antarctic The Antarctic |
genre |
Antarc* Antarctic |
genre_facet |
Antarc* Antarctic |
op_source |
PLoS ONE, Vol 10, Iss 1, p e0116896 (2015) |
op_relation |
http://europepmc.org/articles/PMC4291224?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0116896 https://doaj.org/article/a6ec39363ffe41beb71a1db212030522 |
op_doi |
https://doi.org/10.1371/journal.pone.0116896 |
container_title |
PLOS ONE |
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10 |
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1 |
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e0116896 |
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