Effect of chaotropes in Chagas disease and leishmaniasis cross-reacting serology assays for epidemiological surveys
Abstract INTRODUCTION: Serological cross-reactivity between leishmaniasis and Chagas disease, especially at low titers, leads to difficulties of the seroepidemiological interpretation. METHODS: We have studied the ability of urea as a chaotrope to select high-avidity antibodies in IgG ELISA, thus re...
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ftdoajarticles:oai:doaj.org/article:a6d633656b88483a9a49786b4dfa4aa4 2023-05-15T15:01:19+02:00 Effect of chaotropes in Chagas disease and leishmaniasis cross-reacting serology assays for epidemiological surveys Anderson Pacheco dos Santos Maria Esther de Carvalho Luciana Regina Meirelles Heitor Franco de Andrade Junior https://doi.org/10.1590/0037-8682-0391-2017 https://doaj.org/article/a6d633656b88483a9a49786b4dfa4aa4 EN eng Sociedade Brasileira de Medicina Tropical (SBMT) http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000500665&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0391-2017 https://doaj.org/article/a6d633656b88483a9a49786b4dfa4aa4 Revista da Sociedade Brasileira de Medicina Tropical, Vol 51, Iss 5, Pp 665-669 Serology Chagas disease Leishmaniasis ELISA IFAT IgG antibody avidity Arctic medicine. Tropical medicine RC955-962 article ftdoajarticles https://doi.org/10.1590/0037-8682-0391-2017 2022-12-30T23:22:44Z Abstract INTRODUCTION: Serological cross-reactivity between leishmaniasis and Chagas disease, especially at low titers, leads to difficulties of the seroepidemiological interpretation. METHODS: We have studied the ability of urea as a chaotrope to select high-avidity antibodies in IgG ELISA, thus reducing low-avidity IgG cross-reactivity in serologically positive samples in both assays. RESULTS: Using 0.5M urea for diluting the sample efficiently defined leishmaniasis or double infections in high-avidity IgG ELISA and eliminated false-positive results. CONCLUSIONS: The use of a chaotropic diluting agent is useful for improving the specificity of Chagas disease and leishmaniasis immunoassays. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Revista da Sociedade Brasileira de Medicina Tropical 51 5 665 669 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
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Serology Chagas disease Leishmaniasis ELISA IFAT IgG antibody avidity Arctic medicine. Tropical medicine RC955-962 |
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Serology Chagas disease Leishmaniasis ELISA IFAT IgG antibody avidity Arctic medicine. Tropical medicine RC955-962 Anderson Pacheco dos Santos Maria Esther de Carvalho Luciana Regina Meirelles Heitor Franco de Andrade Junior Effect of chaotropes in Chagas disease and leishmaniasis cross-reacting serology assays for epidemiological surveys |
topic_facet |
Serology Chagas disease Leishmaniasis ELISA IFAT IgG antibody avidity Arctic medicine. Tropical medicine RC955-962 |
description |
Abstract INTRODUCTION: Serological cross-reactivity between leishmaniasis and Chagas disease, especially at low titers, leads to difficulties of the seroepidemiological interpretation. METHODS: We have studied the ability of urea as a chaotrope to select high-avidity antibodies in IgG ELISA, thus reducing low-avidity IgG cross-reactivity in serologically positive samples in both assays. RESULTS: Using 0.5M urea for diluting the sample efficiently defined leishmaniasis or double infections in high-avidity IgG ELISA and eliminated false-positive results. CONCLUSIONS: The use of a chaotropic diluting agent is useful for improving the specificity of Chagas disease and leishmaniasis immunoassays. |
format |
Article in Journal/Newspaper |
author |
Anderson Pacheco dos Santos Maria Esther de Carvalho Luciana Regina Meirelles Heitor Franco de Andrade Junior |
author_facet |
Anderson Pacheco dos Santos Maria Esther de Carvalho Luciana Regina Meirelles Heitor Franco de Andrade Junior |
author_sort |
Anderson Pacheco dos Santos |
title |
Effect of chaotropes in Chagas disease and leishmaniasis cross-reacting serology assays for epidemiological surveys |
title_short |
Effect of chaotropes in Chagas disease and leishmaniasis cross-reacting serology assays for epidemiological surveys |
title_full |
Effect of chaotropes in Chagas disease and leishmaniasis cross-reacting serology assays for epidemiological surveys |
title_fullStr |
Effect of chaotropes in Chagas disease and leishmaniasis cross-reacting serology assays for epidemiological surveys |
title_full_unstemmed |
Effect of chaotropes in Chagas disease and leishmaniasis cross-reacting serology assays for epidemiological surveys |
title_sort |
effect of chaotropes in chagas disease and leishmaniasis cross-reacting serology assays for epidemiological surveys |
publisher |
Sociedade Brasileira de Medicina Tropical (SBMT) |
url |
https://doi.org/10.1590/0037-8682-0391-2017 https://doaj.org/article/a6d633656b88483a9a49786b4dfa4aa4 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
Revista da Sociedade Brasileira de Medicina Tropical, Vol 51, Iss 5, Pp 665-669 |
op_relation |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0037-86822018000500665&lng=en&tlng=en https://doaj.org/toc/1678-9849 1678-9849 doi:10.1590/0037-8682-0391-2017 https://doaj.org/article/a6d633656b88483a9a49786b4dfa4aa4 |
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https://doi.org/10.1590/0037-8682-0391-2017 |
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