Discovery of a novel bat lyssavirus in a Long-fingered bat (Myotis capaccinii) from Slovenia.
Lyssaviruses are the causative agents of rabies, a zoonotic, fatal disease that is thought to be ancestral to bats. In the last decade, the detection of bat associated lyssaviruses is increasing also in Europe. Within a retrospective bat associated lyssavirus surveillance study a total of 225 dead b...
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ftdoajarticles:oai:doaj.org/article:a6d190313afe4b018d5731c740c0d61e 2023-07-30T04:01:53+02:00 Discovery of a novel bat lyssavirus in a Long-fingered bat (Myotis capaccinii) from Slovenia. Danijela Černe Peter Hostnik Ivan Toplak Primož Presetnik Jedrt Maurer-Wernig Urška Kuhar 2023-06-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0011420 https://doaj.org/article/a6d190313afe4b018d5731c740c0d61e EN eng Public Library of Science (PLoS) https://doi.org/10.1371/journal.pntd.0011420 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011420 https://doaj.org/article/a6d190313afe4b018d5731c740c0d61e PLoS Neglected Tropical Diseases, Vol 17, Iss 6, p e0011420 (2023) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2023 ftdoajarticles https://doi.org/10.1371/journal.pntd.0011420 2023-07-09T00:36:33Z Lyssaviruses are the causative agents of rabies, a zoonotic, fatal disease that is thought to be ancestral to bats. In the last decade, the detection of bat associated lyssaviruses is increasing also in Europe. Within a retrospective bat associated lyssavirus surveillance study a total of 225 dead bats of 21 bat species were collected in Slovenia between 2012 and 2019 and tested by specific real-time RT-PCR method. The first lyssavirus positive sample in bats in Slovenia was detected using the real-time RT-PCR, the fluorescent antibody test, and next generation sequencing, while the rabies tissue culture inoculation test was unsuccessful due to sample degradation and storage conditions. The nearly complete genome of Divača bat lyssavirus from Slovenia consists of 11,871 nucleotides and reflects the characteristic gene organization known for lyssaviruses, encoding the five viral proteins. Phylogenetic analysis of Divača bat lyssavirus revealed that it belongs to phylogroup I lyssaviruses and is most closely related to Kotalahti bat lyssavirus (KBLV) with 87.20% nucleotide and 99.22% amino acid identity. Together with KBLV, Khujand virus, European bat lyssavirus 2, Bakeloh bat lyssavirus, and Aravan virus, Divača bat lyssavirus was detected in the genus Myotis suggesting its key role in the transmission and maintenance of certain lyssaviruses. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic Kotalahti ENVELOPE(25.103,25.103,65.570,65.570) PLOS Neglected Tropical Diseases 17 6 e0011420 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Danijela Černe Peter Hostnik Ivan Toplak Primož Presetnik Jedrt Maurer-Wernig Urška Kuhar Discovery of a novel bat lyssavirus in a Long-fingered bat (Myotis capaccinii) from Slovenia. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Lyssaviruses are the causative agents of rabies, a zoonotic, fatal disease that is thought to be ancestral to bats. In the last decade, the detection of bat associated lyssaviruses is increasing also in Europe. Within a retrospective bat associated lyssavirus surveillance study a total of 225 dead bats of 21 bat species were collected in Slovenia between 2012 and 2019 and tested by specific real-time RT-PCR method. The first lyssavirus positive sample in bats in Slovenia was detected using the real-time RT-PCR, the fluorescent antibody test, and next generation sequencing, while the rabies tissue culture inoculation test was unsuccessful due to sample degradation and storage conditions. The nearly complete genome of Divača bat lyssavirus from Slovenia consists of 11,871 nucleotides and reflects the characteristic gene organization known for lyssaviruses, encoding the five viral proteins. Phylogenetic analysis of Divača bat lyssavirus revealed that it belongs to phylogroup I lyssaviruses and is most closely related to Kotalahti bat lyssavirus (KBLV) with 87.20% nucleotide and 99.22% amino acid identity. Together with KBLV, Khujand virus, European bat lyssavirus 2, Bakeloh bat lyssavirus, and Aravan virus, Divača bat lyssavirus was detected in the genus Myotis suggesting its key role in the transmission and maintenance of certain lyssaviruses. |
format |
Article in Journal/Newspaper |
author |
Danijela Černe Peter Hostnik Ivan Toplak Primož Presetnik Jedrt Maurer-Wernig Urška Kuhar |
author_facet |
Danijela Černe Peter Hostnik Ivan Toplak Primož Presetnik Jedrt Maurer-Wernig Urška Kuhar |
author_sort |
Danijela Černe |
title |
Discovery of a novel bat lyssavirus in a Long-fingered bat (Myotis capaccinii) from Slovenia. |
title_short |
Discovery of a novel bat lyssavirus in a Long-fingered bat (Myotis capaccinii) from Slovenia. |
title_full |
Discovery of a novel bat lyssavirus in a Long-fingered bat (Myotis capaccinii) from Slovenia. |
title_fullStr |
Discovery of a novel bat lyssavirus in a Long-fingered bat (Myotis capaccinii) from Slovenia. |
title_full_unstemmed |
Discovery of a novel bat lyssavirus in a Long-fingered bat (Myotis capaccinii) from Slovenia. |
title_sort |
discovery of a novel bat lyssavirus in a long-fingered bat (myotis capaccinii) from slovenia. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2023 |
url |
https://doi.org/10.1371/journal.pntd.0011420 https://doaj.org/article/a6d190313afe4b018d5731c740c0d61e |
long_lat |
ENVELOPE(25.103,25.103,65.570,65.570) |
geographic |
Arctic Kotalahti |
geographic_facet |
Arctic Kotalahti |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 17, Iss 6, p e0011420 (2023) |
op_relation |
https://doi.org/10.1371/journal.pntd.0011420 https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0011420 https://doaj.org/article/a6d190313afe4b018d5731c740c0d61e |
op_doi |
https://doi.org/10.1371/journal.pntd.0011420 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
17 |
container_issue |
6 |
container_start_page |
e0011420 |
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1772812627323387904 |