Production of Lipopeptide Biosurfactant by a Hydrocarbon-Degrading Antarctic Rhodococcus
Rhodococci are renowned for their great metabolic repertoire partly because of their numerous putative pathways for large number of specialized metabolites such as biosurfactant. Screening and genome-based assessment for the capacity to produce surface-active molecules was conducted on Rhodococcus s...
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ftdoajarticles:oai:doaj.org/article:a6c52858f71a4e9dbc9f13360fba19c0 2023-05-15T13:52:41+02:00 Production of Lipopeptide Biosurfactant by a Hydrocarbon-Degrading Antarctic Rhodococcus Syahir Habib Siti Aqlima Ahmad Wan Lutfi Wan Johari Mohd Yunus Abd Shukor Siti Aisyah Alias Jerzy Smykla Nurul Hani Saruni Nur Syafiqah Abdul Razak Nur Adeela Yasid 2020-08-01T00:00:00Z https://doi.org/10.3390/ijms21176138 https://doaj.org/article/a6c52858f71a4e9dbc9f13360fba19c0 EN eng MDPI AG https://www.mdpi.com/1422-0067/21/17/6138 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms21176138 1422-0067 1661-6596 https://doaj.org/article/a6c52858f71a4e9dbc9f13360fba19c0 International Journal of Molecular Sciences, Vol 21, Iss 6138, p 6138 (2020) surface-active lipopeptide biosynthetic gene clusters fellfield soil Antarctica Biology (General) QH301-705.5 Chemistry QD1-999 article 2020 ftdoajarticles https://doi.org/10.3390/ijms21176138 2022-12-31T07:43:52Z Rhodococci are renowned for their great metabolic repertoire partly because of their numerous putative pathways for large number of specialized metabolites such as biosurfactant. Screening and genome-based assessment for the capacity to produce surface-active molecules was conducted on Rhodococcus sp. ADL36, a diesel-degrading Antarctic bacterium. The strain showed a positive bacterial adhesion to hydrocarbon (BATH) assay, drop collapse test, oil displacement activity, microplate assay, maximal emulsification index at 45% and ability to reduce water surface tension to < 30 mN/m. The evaluation of the cell-free supernatant demonstrated its high stability across the temperature, pH and salinity gradient although no correlation was found between the surface and emulsification activity. Based on the positive relationship between the assessment of macromolecules content and infrared analysis, the extracted biosurfactant synthesized was classified as a lipopeptide. Prediction of the secondary metabolites in the non-ribosomal peptide synthetase (NRPS) clusters suggested the likelihood of the surface-active lipopeptide production in the strain’s genomic data. This is the third report of surface-active lipopeptide producers from this phylotype and the first from the polar region. The lipopeptide synthesized by ADL36 has the prospect to be an Antarctic remediation tool while furnishing a distinctive natural product for biotechnological application and research. Article in Journal/Newspaper Antarc* Antarctic Antarctica Directory of Open Access Journals: DOAJ Articles Antarctic International Journal of Molecular Sciences 21 17 6138 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
surface-active lipopeptide biosynthetic gene clusters fellfield soil Antarctica Biology (General) QH301-705.5 Chemistry QD1-999 |
spellingShingle |
surface-active lipopeptide biosynthetic gene clusters fellfield soil Antarctica Biology (General) QH301-705.5 Chemistry QD1-999 Syahir Habib Siti Aqlima Ahmad Wan Lutfi Wan Johari Mohd Yunus Abd Shukor Siti Aisyah Alias Jerzy Smykla Nurul Hani Saruni Nur Syafiqah Abdul Razak Nur Adeela Yasid Production of Lipopeptide Biosurfactant by a Hydrocarbon-Degrading Antarctic Rhodococcus |
topic_facet |
surface-active lipopeptide biosynthetic gene clusters fellfield soil Antarctica Biology (General) QH301-705.5 Chemistry QD1-999 |
description |
Rhodococci are renowned for their great metabolic repertoire partly because of their numerous putative pathways for large number of specialized metabolites such as biosurfactant. Screening and genome-based assessment for the capacity to produce surface-active molecules was conducted on Rhodococcus sp. ADL36, a diesel-degrading Antarctic bacterium. The strain showed a positive bacterial adhesion to hydrocarbon (BATH) assay, drop collapse test, oil displacement activity, microplate assay, maximal emulsification index at 45% and ability to reduce water surface tension to < 30 mN/m. The evaluation of the cell-free supernatant demonstrated its high stability across the temperature, pH and salinity gradient although no correlation was found between the surface and emulsification activity. Based on the positive relationship between the assessment of macromolecules content and infrared analysis, the extracted biosurfactant synthesized was classified as a lipopeptide. Prediction of the secondary metabolites in the non-ribosomal peptide synthetase (NRPS) clusters suggested the likelihood of the surface-active lipopeptide production in the strain’s genomic data. This is the third report of surface-active lipopeptide producers from this phylotype and the first from the polar region. The lipopeptide synthesized by ADL36 has the prospect to be an Antarctic remediation tool while furnishing a distinctive natural product for biotechnological application and research. |
format |
Article in Journal/Newspaper |
author |
Syahir Habib Siti Aqlima Ahmad Wan Lutfi Wan Johari Mohd Yunus Abd Shukor Siti Aisyah Alias Jerzy Smykla Nurul Hani Saruni Nur Syafiqah Abdul Razak Nur Adeela Yasid |
author_facet |
Syahir Habib Siti Aqlima Ahmad Wan Lutfi Wan Johari Mohd Yunus Abd Shukor Siti Aisyah Alias Jerzy Smykla Nurul Hani Saruni Nur Syafiqah Abdul Razak Nur Adeela Yasid |
author_sort |
Syahir Habib |
title |
Production of Lipopeptide Biosurfactant by a Hydrocarbon-Degrading Antarctic Rhodococcus |
title_short |
Production of Lipopeptide Biosurfactant by a Hydrocarbon-Degrading Antarctic Rhodococcus |
title_full |
Production of Lipopeptide Biosurfactant by a Hydrocarbon-Degrading Antarctic Rhodococcus |
title_fullStr |
Production of Lipopeptide Biosurfactant by a Hydrocarbon-Degrading Antarctic Rhodococcus |
title_full_unstemmed |
Production of Lipopeptide Biosurfactant by a Hydrocarbon-Degrading Antarctic Rhodococcus |
title_sort |
production of lipopeptide biosurfactant by a hydrocarbon-degrading antarctic rhodococcus |
publisher |
MDPI AG |
publishDate |
2020 |
url |
https://doi.org/10.3390/ijms21176138 https://doaj.org/article/a6c52858f71a4e9dbc9f13360fba19c0 |
geographic |
Antarctic |
geographic_facet |
Antarctic |
genre |
Antarc* Antarctic Antarctica |
genre_facet |
Antarc* Antarctic Antarctica |
op_source |
International Journal of Molecular Sciences, Vol 21, Iss 6138, p 6138 (2020) |
op_relation |
https://www.mdpi.com/1422-0067/21/17/6138 https://doaj.org/toc/1661-6596 https://doaj.org/toc/1422-0067 doi:10.3390/ijms21176138 1422-0067 1661-6596 https://doaj.org/article/a6c52858f71a4e9dbc9f13360fba19c0 |
op_doi |
https://doi.org/10.3390/ijms21176138 |
container_title |
International Journal of Molecular Sciences |
container_volume |
21 |
container_issue |
17 |
container_start_page |
6138 |
_version_ |
1766257106832326656 |