Carbonic anhydrase is involved in calcification by the benthic foraminifer Amphistegina lessonii
Marine calcification is an important component of the global carbon cycle. The mechanism by which some organisms take up inorganic carbon for the production of their shells or skeletons, however, remains only partly known. Although foraminifera are responsible for a large part of the global calcium...
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ftdoajarticles:oai:doaj.org/article:a6b30453435046d8a0e4e409be31d57f 2023-05-15T17:51:31+02:00 Carbonic anhydrase is involved in calcification by the benthic foraminifer Amphistegina lessonii S. de Goeyse A. E. Webb G.-J. Reichart L. J. de Nooijer 2021-01-01T00:00:00Z https://doi.org/10.5194/bg-18-393-2021 https://doaj.org/article/a6b30453435046d8a0e4e409be31d57f EN eng Copernicus Publications https://bg.copernicus.org/articles/18/393/2021/bg-18-393-2021.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 doi:10.5194/bg-18-393-2021 1726-4170 1726-4189 https://doaj.org/article/a6b30453435046d8a0e4e409be31d57f Biogeosciences, Vol 18, Pp 393-401 (2021) Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 article 2021 ftdoajarticles https://doi.org/10.5194/bg-18-393-2021 2022-12-31T06:00:17Z Marine calcification is an important component of the global carbon cycle. The mechanism by which some organisms take up inorganic carbon for the production of their shells or skeletons, however, remains only partly known. Although foraminifera are responsible for a large part of the global calcium carbonate production, the process by which they concentrate inorganic carbon is debated. Some evidence suggests that seawater is taken up by vacuolization and participates relatively unaltered in the process of calcification, whereas other results suggest the involvement of transmembrane transport and the activity of enzymes like carbonic anhydrase. Here, we tested whether inorganic-carbon uptake relies on the activity of carbonic anhydrase using incubation experiments with the perforate, large benthic, symbiont-bearing foraminifer Amphistegina lessonii . Calcification rates, determined by the alkalinity anomaly method, showed that inhibition of carbonic anhydrase by acetazolamide (AZ) stopped most of the calcification process. Inhibition of photosynthesis either by 3-(3,4-Dichlorophenyl)-1,1-dimethylurea (DCMU) or by incubating the foraminifera in the dark also decreased calcification rates but to a lesser degree than with AZ. Results from this study show that carbonic anhydrase plays a key role in biomineralization of Amphistegina lessonii and indicates that calcification of those perforate, large benthic foraminifera might, to a certain extent, benefit from the extra dissolved inorganic carbon (DIC), which causes ocean acidification. Article in Journal/Newspaper Ocean acidification Directory of Open Access Journals: DOAJ Articles Biogeosciences 18 2 393 401 |
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Directory of Open Access Journals: DOAJ Articles |
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English |
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Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
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Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 S. de Goeyse A. E. Webb G.-J. Reichart L. J. de Nooijer Carbonic anhydrase is involved in calcification by the benthic foraminifer Amphistegina lessonii |
topic_facet |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
description |
Marine calcification is an important component of the global carbon cycle. The mechanism by which some organisms take up inorganic carbon for the production of their shells or skeletons, however, remains only partly known. Although foraminifera are responsible for a large part of the global calcium carbonate production, the process by which they concentrate inorganic carbon is debated. Some evidence suggests that seawater is taken up by vacuolization and participates relatively unaltered in the process of calcification, whereas other results suggest the involvement of transmembrane transport and the activity of enzymes like carbonic anhydrase. Here, we tested whether inorganic-carbon uptake relies on the activity of carbonic anhydrase using incubation experiments with the perforate, large benthic, symbiont-bearing foraminifer Amphistegina lessonii . Calcification rates, determined by the alkalinity anomaly method, showed that inhibition of carbonic anhydrase by acetazolamide (AZ) stopped most of the calcification process. Inhibition of photosynthesis either by 3-(3,4-Dichlorophenyl)-1,1-dimethylurea (DCMU) or by incubating the foraminifera in the dark also decreased calcification rates but to a lesser degree than with AZ. Results from this study show that carbonic anhydrase plays a key role in biomineralization of Amphistegina lessonii and indicates that calcification of those perforate, large benthic foraminifera might, to a certain extent, benefit from the extra dissolved inorganic carbon (DIC), which causes ocean acidification. |
format |
Article in Journal/Newspaper |
author |
S. de Goeyse A. E. Webb G.-J. Reichart L. J. de Nooijer |
author_facet |
S. de Goeyse A. E. Webb G.-J. Reichart L. J. de Nooijer |
author_sort |
S. de Goeyse |
title |
Carbonic anhydrase is involved in calcification by the benthic foraminifer Amphistegina lessonii |
title_short |
Carbonic anhydrase is involved in calcification by the benthic foraminifer Amphistegina lessonii |
title_full |
Carbonic anhydrase is involved in calcification by the benthic foraminifer Amphistegina lessonii |
title_fullStr |
Carbonic anhydrase is involved in calcification by the benthic foraminifer Amphistegina lessonii |
title_full_unstemmed |
Carbonic anhydrase is involved in calcification by the benthic foraminifer Amphistegina lessonii |
title_sort |
carbonic anhydrase is involved in calcification by the benthic foraminifer amphistegina lessonii |
publisher |
Copernicus Publications |
publishDate |
2021 |
url |
https://doi.org/10.5194/bg-18-393-2021 https://doaj.org/article/a6b30453435046d8a0e4e409be31d57f |
genre |
Ocean acidification |
genre_facet |
Ocean acidification |
op_source |
Biogeosciences, Vol 18, Pp 393-401 (2021) |
op_relation |
https://bg.copernicus.org/articles/18/393/2021/bg-18-393-2021.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 doi:10.5194/bg-18-393-2021 1726-4170 1726-4189 https://doaj.org/article/a6b30453435046d8a0e4e409be31d57f |
op_doi |
https://doi.org/10.5194/bg-18-393-2021 |
container_title |
Biogeosciences |
container_volume |
18 |
container_issue |
2 |
container_start_page |
393 |
op_container_end_page |
401 |
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1766158698301882368 |