Tracing of the Bile-chemotactic migration of juvenile Clonorchis sinensis in rabbits by PET-CT.
BACKGROUND: Adult Clonorchis sinensis live in the bile duct and cause clonorchiasis. It is known that the C. sinensis metacercariae excyst in the duodenum and migrate up to the bile duct through the common bile duct. However, no direct evidence is available on the in vivo migration of newly excysted...
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ftdoajarticles:oai:doaj.org/article:a66a9af1f1b64892b6a5fc37d7cd2b20 2023-05-15T15:14:29+02:00 Tracing of the Bile-chemotactic migration of juvenile Clonorchis sinensis in rabbits by PET-CT. Tae Im Kim Won Gi Yoo Byung Kook Kwak Ju-Won Seok Sung-Jong Hong 2011-12-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0001414 https://doaj.org/article/a66a9af1f1b64892b6a5fc37d7cd2b20 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC3236719?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001414 https://doaj.org/article/a66a9af1f1b64892b6a5fc37d7cd2b20 PLoS Neglected Tropical Diseases, Vol 5, Iss 12, p e1414 (2011) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2011 ftdoajarticles https://doi.org/10.1371/journal.pntd.0001414 2022-12-31T02:09:11Z BACKGROUND: Adult Clonorchis sinensis live in the bile duct and cause clonorchiasis. It is known that the C. sinensis metacercariae excyst in the duodenum and migrate up to the bile duct through the common bile duct. However, no direct evidence is available on the in vivo migration of newly excysted C. sinensis juveniles (CsNEJs). Advanced imaging technologies now allow the in vivo migration and localization to be visualized. In the present study, we sought to determine how sensitively CsNEJs respond to bile and how fast they migrate to the intrahepatic bile duct using PET-CT. METHODOLOGY/PRINCIPAL FINDINGS: CsNEJs were radiolabeled with (18)F-fluorodeoxyglucose ((18)F-FDG). Rabbits with a gallbladder contraction response to cholecystokinin-8 (CCK-8) injection were pre-screened using cholescintigraphy. In these rabbits, gallbladders contracted by 50% in volume at an average of 11.5 min post-injection. The four rabbits examined were kept anesthetized and a catheter inserted into the mid duodenum. Gallbladder contraction was stimulated by injecting CCK-8 (20 ng/kg every minute) over the experiment. Anatomical images were acquired by CT initially and dynamic PET was then carried out for 90 min with a 3-min acquisition per frame. Twelve minutes after CCK-8 injection, about 3,000 (18)F-FDG-labeled CsNEJs were inoculated into the mid duodenum through the catheter. Photon signals were detected in the liver 7-9 min after CsNEJs inoculation, and these then increased in the whole liver with stronger intensity in the central area, presenting that the CsNEJs were arriving at the intrahepatic bile ducts. CONCLUSION: In the duodenum, CsNEJs immediately sense bile and migrate quickly with bile-chemotaxis to reach the intrahepatic bile ducts by way of the ampulla of Vater. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 5 12 e1414 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
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English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Tae Im Kim Won Gi Yoo Byung Kook Kwak Ju-Won Seok Sung-Jong Hong Tracing of the Bile-chemotactic migration of juvenile Clonorchis sinensis in rabbits by PET-CT. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND: Adult Clonorchis sinensis live in the bile duct and cause clonorchiasis. It is known that the C. sinensis metacercariae excyst in the duodenum and migrate up to the bile duct through the common bile duct. However, no direct evidence is available on the in vivo migration of newly excysted C. sinensis juveniles (CsNEJs). Advanced imaging technologies now allow the in vivo migration and localization to be visualized. In the present study, we sought to determine how sensitively CsNEJs respond to bile and how fast they migrate to the intrahepatic bile duct using PET-CT. METHODOLOGY/PRINCIPAL FINDINGS: CsNEJs were radiolabeled with (18)F-fluorodeoxyglucose ((18)F-FDG). Rabbits with a gallbladder contraction response to cholecystokinin-8 (CCK-8) injection were pre-screened using cholescintigraphy. In these rabbits, gallbladders contracted by 50% in volume at an average of 11.5 min post-injection. The four rabbits examined were kept anesthetized and a catheter inserted into the mid duodenum. Gallbladder contraction was stimulated by injecting CCK-8 (20 ng/kg every minute) over the experiment. Anatomical images were acquired by CT initially and dynamic PET was then carried out for 90 min with a 3-min acquisition per frame. Twelve minutes after CCK-8 injection, about 3,000 (18)F-FDG-labeled CsNEJs were inoculated into the mid duodenum through the catheter. Photon signals were detected in the liver 7-9 min after CsNEJs inoculation, and these then increased in the whole liver with stronger intensity in the central area, presenting that the CsNEJs were arriving at the intrahepatic bile ducts. CONCLUSION: In the duodenum, CsNEJs immediately sense bile and migrate quickly with bile-chemotaxis to reach the intrahepatic bile ducts by way of the ampulla of Vater. |
format |
Article in Journal/Newspaper |
author |
Tae Im Kim Won Gi Yoo Byung Kook Kwak Ju-Won Seok Sung-Jong Hong |
author_facet |
Tae Im Kim Won Gi Yoo Byung Kook Kwak Ju-Won Seok Sung-Jong Hong |
author_sort |
Tae Im Kim |
title |
Tracing of the Bile-chemotactic migration of juvenile Clonorchis sinensis in rabbits by PET-CT. |
title_short |
Tracing of the Bile-chemotactic migration of juvenile Clonorchis sinensis in rabbits by PET-CT. |
title_full |
Tracing of the Bile-chemotactic migration of juvenile Clonorchis sinensis in rabbits by PET-CT. |
title_fullStr |
Tracing of the Bile-chemotactic migration of juvenile Clonorchis sinensis in rabbits by PET-CT. |
title_full_unstemmed |
Tracing of the Bile-chemotactic migration of juvenile Clonorchis sinensis in rabbits by PET-CT. |
title_sort |
tracing of the bile-chemotactic migration of juvenile clonorchis sinensis in rabbits by pet-ct. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2011 |
url |
https://doi.org/10.1371/journal.pntd.0001414 https://doaj.org/article/a66a9af1f1b64892b6a5fc37d7cd2b20 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 5, Iss 12, p e1414 (2011) |
op_relation |
http://europepmc.org/articles/PMC3236719?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0001414 https://doaj.org/article/a66a9af1f1b64892b6a5fc37d7cd2b20 |
op_doi |
https://doi.org/10.1371/journal.pntd.0001414 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
5 |
container_issue |
12 |
container_start_page |
e1414 |
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1766344932049551360 |