Functional characterization of bacteria isolated from ancient arctic soil exposes diverse resistance mechanisms to modern antibiotics.
Using functional metagenomics to study the resistomes of bacterial communities isolated from different layers of the Canadian high Arctic permafrost, we show that microbial communities harbored diverse resistance mechanisms at least 5,000 years ago. Among bacteria sampled from the ancient layers of...
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ftdoajarticles:oai:doaj.org/article:a5eaa9009e454752a1813fc793fbbace 2023-05-15T14:57:07+02:00 Functional characterization of bacteria isolated from ancient arctic soil exposes diverse resistance mechanisms to modern antibiotics. Gabriel G Perron Lyle Whyte Peter J Turnbaugh Jacqueline Goordial William P Hanage Gautam Dantas Michael M Desai 2015-01-01T00:00:00Z https://doi.org/10.1371/journal.pone.0069533 https://doaj.org/article/a5eaa9009e454752a1813fc793fbbace EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4373940?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0069533 https://doaj.org/article/a5eaa9009e454752a1813fc793fbbace PLoS ONE, Vol 10, Iss 3, p e0069533 (2015) Medicine R Science Q article 2015 ftdoajarticles https://doi.org/10.1371/journal.pone.0069533 2022-12-31T13:49:35Z Using functional metagenomics to study the resistomes of bacterial communities isolated from different layers of the Canadian high Arctic permafrost, we show that microbial communities harbored diverse resistance mechanisms at least 5,000 years ago. Among bacteria sampled from the ancient layers of a permafrost core, we isolated eight genes conferring clinical levels of resistance against aminoglycoside, β-lactam and tetracycline antibiotics that are naturally produced by microorganisms. Among these resistance genes, four also conferred resistance against amikacin, a modern semi-synthetic antibiotic that does not naturally occur in microorganisms. In bacteria sampled from the overlaying active layer, we isolated ten different genes conferring resistance to all six antibiotics tested in this study, including aminoglycoside, β-lactam and tetracycline variants that are naturally produced by microorganisms as well as semi-synthetic variants produced in the laboratory. On average, we found that resistance genes found in permafrost bacteria conferred lower levels of resistance against clinically relevant antibiotics than resistance genes sampled from the active layer. Our results demonstrate that antibiotic resistance genes were functionally diverse prior to the anthropogenic use of antibiotics, contributing to the evolution of natural reservoirs of resistance genes. Article in Journal/Newspaper Arctic permafrost Directory of Open Access Journals: DOAJ Articles Arctic PLOS ONE 10 3 e0069533 |
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Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Medicine R Science Q |
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Medicine R Science Q Gabriel G Perron Lyle Whyte Peter J Turnbaugh Jacqueline Goordial William P Hanage Gautam Dantas Michael M Desai Functional characterization of bacteria isolated from ancient arctic soil exposes diverse resistance mechanisms to modern antibiotics. |
topic_facet |
Medicine R Science Q |
description |
Using functional metagenomics to study the resistomes of bacterial communities isolated from different layers of the Canadian high Arctic permafrost, we show that microbial communities harbored diverse resistance mechanisms at least 5,000 years ago. Among bacteria sampled from the ancient layers of a permafrost core, we isolated eight genes conferring clinical levels of resistance against aminoglycoside, β-lactam and tetracycline antibiotics that are naturally produced by microorganisms. Among these resistance genes, four also conferred resistance against amikacin, a modern semi-synthetic antibiotic that does not naturally occur in microorganisms. In bacteria sampled from the overlaying active layer, we isolated ten different genes conferring resistance to all six antibiotics tested in this study, including aminoglycoside, β-lactam and tetracycline variants that are naturally produced by microorganisms as well as semi-synthetic variants produced in the laboratory. On average, we found that resistance genes found in permafrost bacteria conferred lower levels of resistance against clinically relevant antibiotics than resistance genes sampled from the active layer. Our results demonstrate that antibiotic resistance genes were functionally diverse prior to the anthropogenic use of antibiotics, contributing to the evolution of natural reservoirs of resistance genes. |
format |
Article in Journal/Newspaper |
author |
Gabriel G Perron Lyle Whyte Peter J Turnbaugh Jacqueline Goordial William P Hanage Gautam Dantas Michael M Desai |
author_facet |
Gabriel G Perron Lyle Whyte Peter J Turnbaugh Jacqueline Goordial William P Hanage Gautam Dantas Michael M Desai |
author_sort |
Gabriel G Perron |
title |
Functional characterization of bacteria isolated from ancient arctic soil exposes diverse resistance mechanisms to modern antibiotics. |
title_short |
Functional characterization of bacteria isolated from ancient arctic soil exposes diverse resistance mechanisms to modern antibiotics. |
title_full |
Functional characterization of bacteria isolated from ancient arctic soil exposes diverse resistance mechanisms to modern antibiotics. |
title_fullStr |
Functional characterization of bacteria isolated from ancient arctic soil exposes diverse resistance mechanisms to modern antibiotics. |
title_full_unstemmed |
Functional characterization of bacteria isolated from ancient arctic soil exposes diverse resistance mechanisms to modern antibiotics. |
title_sort |
functional characterization of bacteria isolated from ancient arctic soil exposes diverse resistance mechanisms to modern antibiotics. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pone.0069533 https://doaj.org/article/a5eaa9009e454752a1813fc793fbbace |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic permafrost |
genre_facet |
Arctic permafrost |
op_source |
PLoS ONE, Vol 10, Iss 3, p e0069533 (2015) |
op_relation |
http://europepmc.org/articles/PMC4373940?pdf=render https://doaj.org/toc/1932-6203 1932-6203 doi:10.1371/journal.pone.0069533 https://doaj.org/article/a5eaa9009e454752a1813fc793fbbace |
op_doi |
https://doi.org/10.1371/journal.pone.0069533 |
container_title |
PLOS ONE |
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10 |
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3 |
container_start_page |
e0069533 |
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1766329208231952384 |