Biodistribution and trafficking of hydrogel nanoparticles in adult mosquitoes.
BACKGROUND:Nanotechnology offers great potential for molecular genetic investigations and potential control of medically important arthropods. Major advances have been made in mammalian systems to define nanoparticle (NP) characteristics that condition trafficking and biodistribution of NPs in the h...
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ftdoajarticles:oai:doaj.org/article:a5da1606b18b440580ffa96192e587c8 2023-05-15T15:12:54+02:00 Biodistribution and trafficking of hydrogel nanoparticles in adult mosquitoes. Cynthia C H Paquette Yashdeep Phanse Jillian L Perry Irma Sanchez-Vargas Paul M Airs Brendan M Dunphy Jing Xu Jonathan O Carlson J Christopher Luft Joseph M DeSimone Lyric C Bartholomay Barry J Beaty 2015-05-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0003745 https://doaj.org/article/a5da1606b18b440580ffa96192e587c8 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4440717?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003745 https://doaj.org/article/a5da1606b18b440580ffa96192e587c8 PLoS Neglected Tropical Diseases, Vol 9, Iss 5, p e0003745 (2015) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2015 ftdoajarticles https://doi.org/10.1371/journal.pntd.0003745 2022-12-31T03:22:24Z BACKGROUND:Nanotechnology offers great potential for molecular genetic investigations and potential control of medically important arthropods. Major advances have been made in mammalian systems to define nanoparticle (NP) characteristics that condition trafficking and biodistribution of NPs in the host. Such information is critical for effective delivery of therapeutics and molecules to cells and organs, but little is known about biodistribution of NPs in mosquitoes. METHODOLOGY/PRINCIPAL FINDINGS:PRINT technology was used to construct a library of fluorescently labeled hydrogel NPs of defined size, shape, and surface charge. The biodistribution (organ, tissue, and cell tropisms and trafficking kinetics) of positively and negatively charged 200 nm x 200 nm, 80 nm x 320 nm, and 80 nm x 5000 nm NPs was determined in adult Anopheles gambiae mosquitoes as a function of the route of challenge (ingestion, injection or contact) using whole body imaging and fluorescence microscopy. Mosquitoes readily ingested NPs in sugar solution. Whole body fluorescence imaging revealed substantial NP accumulation (load) in the alimentary tracts of the adult mosquitoes, with the greatest loads in the diverticula, cardia and foregut. Positively and negatively charged NPs differed in their biodistribution and trafficking. Following oral challenge, negatively charged NPs transited the alimentary tract more rapidly than positively charged NPs. Following contact challenge, negatively charged NPs trafficked more efficiently in alimentary tract tissues. Following parenteral challenge, positively and negatively charged NPs differed in tissue tropisms and trafficking in the hemocoel. Injected NPs were also detected in cardia/foregut, suggesting trafficking of NPs from the hemocoel into the alimentary tract. CONCLUSIONS/SIGNIFICANCE:Herein we have developed a tool box of NPs with the biodistribution and tissue tropism characteristics for gene structure/function studies and for delivery of vector lethal cargoes for mosquito control. Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 9 5 e0003745 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
spellingShingle |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Cynthia C H Paquette Yashdeep Phanse Jillian L Perry Irma Sanchez-Vargas Paul M Airs Brendan M Dunphy Jing Xu Jonathan O Carlson J Christopher Luft Joseph M DeSimone Lyric C Bartholomay Barry J Beaty Biodistribution and trafficking of hydrogel nanoparticles in adult mosquitoes. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:Nanotechnology offers great potential for molecular genetic investigations and potential control of medically important arthropods. Major advances have been made in mammalian systems to define nanoparticle (NP) characteristics that condition trafficking and biodistribution of NPs in the host. Such information is critical for effective delivery of therapeutics and molecules to cells and organs, but little is known about biodistribution of NPs in mosquitoes. METHODOLOGY/PRINCIPAL FINDINGS:PRINT technology was used to construct a library of fluorescently labeled hydrogel NPs of defined size, shape, and surface charge. The biodistribution (organ, tissue, and cell tropisms and trafficking kinetics) of positively and negatively charged 200 nm x 200 nm, 80 nm x 320 nm, and 80 nm x 5000 nm NPs was determined in adult Anopheles gambiae mosquitoes as a function of the route of challenge (ingestion, injection or contact) using whole body imaging and fluorescence microscopy. Mosquitoes readily ingested NPs in sugar solution. Whole body fluorescence imaging revealed substantial NP accumulation (load) in the alimentary tracts of the adult mosquitoes, with the greatest loads in the diverticula, cardia and foregut. Positively and negatively charged NPs differed in their biodistribution and trafficking. Following oral challenge, negatively charged NPs transited the alimentary tract more rapidly than positively charged NPs. Following contact challenge, negatively charged NPs trafficked more efficiently in alimentary tract tissues. Following parenteral challenge, positively and negatively charged NPs differed in tissue tropisms and trafficking in the hemocoel. Injected NPs were also detected in cardia/foregut, suggesting trafficking of NPs from the hemocoel into the alimentary tract. CONCLUSIONS/SIGNIFICANCE:Herein we have developed a tool box of NPs with the biodistribution and tissue tropism characteristics for gene structure/function studies and for delivery of vector lethal cargoes for mosquito control. |
format |
Article in Journal/Newspaper |
author |
Cynthia C H Paquette Yashdeep Phanse Jillian L Perry Irma Sanchez-Vargas Paul M Airs Brendan M Dunphy Jing Xu Jonathan O Carlson J Christopher Luft Joseph M DeSimone Lyric C Bartholomay Barry J Beaty |
author_facet |
Cynthia C H Paquette Yashdeep Phanse Jillian L Perry Irma Sanchez-Vargas Paul M Airs Brendan M Dunphy Jing Xu Jonathan O Carlson J Christopher Luft Joseph M DeSimone Lyric C Bartholomay Barry J Beaty |
author_sort |
Cynthia C H Paquette |
title |
Biodistribution and trafficking of hydrogel nanoparticles in adult mosquitoes. |
title_short |
Biodistribution and trafficking of hydrogel nanoparticles in adult mosquitoes. |
title_full |
Biodistribution and trafficking of hydrogel nanoparticles in adult mosquitoes. |
title_fullStr |
Biodistribution and trafficking of hydrogel nanoparticles in adult mosquitoes. |
title_full_unstemmed |
Biodistribution and trafficking of hydrogel nanoparticles in adult mosquitoes. |
title_sort |
biodistribution and trafficking of hydrogel nanoparticles in adult mosquitoes. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2015 |
url |
https://doi.org/10.1371/journal.pntd.0003745 https://doaj.org/article/a5da1606b18b440580ffa96192e587c8 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 9, Iss 5, p e0003745 (2015) |
op_relation |
http://europepmc.org/articles/PMC4440717?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0003745 https://doaj.org/article/a5da1606b18b440580ffa96192e587c8 |
op_doi |
https://doi.org/10.1371/journal.pntd.0003745 |
container_title |
PLOS Neglected Tropical Diseases |
container_volume |
9 |
container_issue |
5 |
container_start_page |
e0003745 |
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