Taxon-specific responses of Southern Ocean diatoms to Fe enrichment revealed by synchrotron radiation FTIR microspectroscopy
Photosynthesis by marine diatoms contributes substantially to global biogeochemical cycling and ecosystem productivity. It is widely accepted that diatoms are extremely sensitive to changes in Fe availability, with numerous in situ experiments demonstrating rapid growth and increased export of eleme...
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ftdoajarticles:oai:doaj.org/article:a5d62fdfa60d4cd4b7fe4cf162a963a8 2023-05-15T18:25:16+02:00 Taxon-specific responses of Southern Ocean diatoms to Fe enrichment revealed by synchrotron radiation FTIR microspectroscopy O. Sackett L. Armand J. Beardall R. Hill M. Doblin C. Connelly J. Howes B. Stuart P. Ralph P. Heraud 2014-10-01T00:00:00Z https://doi.org/10.5194/bg-11-5795-2014 https://doaj.org/article/a5d62fdfa60d4cd4b7fe4cf162a963a8 EN eng Copernicus Publications http://www.biogeosciences.net/11/5795/2014/bg-11-5795-2014.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 1726-4170 1726-4189 doi:10.5194/bg-11-5795-2014 https://doaj.org/article/a5d62fdfa60d4cd4b7fe4cf162a963a8 Biogeosciences, Vol 11, Iss 20, Pp 5795-5808 (2014) Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 article 2014 ftdoajarticles https://doi.org/10.5194/bg-11-5795-2014 2022-12-30T23:38:34Z Photosynthesis by marine diatoms contributes substantially to global biogeochemical cycling and ecosystem productivity. It is widely accepted that diatoms are extremely sensitive to changes in Fe availability, with numerous in situ experiments demonstrating rapid growth and increased export of elements (e.g. C, Si and Fe) from surface waters as a result of Fe addition. Less is known about the effects of Fe enrichment on the phenotypes of diatoms, such as associated changes in nutritional value – furthermore, data on taxon-specific responses are almost non-existent. Enhanced supply of nutrient-rich waters along the coast of the subantarctic Kerguelen Island provide a valuable opportunity to examine the responses of phytoplankton to natural Fe enrichment. Here we demonstrate the use of synchrotron radiation Fourier Transform Infrared (SR-FTIR) microspectroscopy to analyse changes in the macromolecular composition of diatoms collected along the coast and plateau of Kerguelen Island, Southern Ocean. SR-FTIR microspectroscopy enabled the analysis of individual diatom cells from mixed communities of field-collected samples, thereby providing insight into in situ taxon-specific responses in relation to changes in Fe availability. Phenotypic responses were taxon-specific in terms of intraspecific variability and changes in proteins, amino acids, phosphorylated molecules, silicate/silicic acid and carbohydrates. In contrast to some previous studies, silicate/silicic acid levels increased under Fe enrichment, in conjunction with increases in carbohydrate stores. The highly abundant taxon Fragilariopsis kerguelensis displayed a higher level of phenotypic plasticity than Pseudo-nitzschia spp., while analysis of the data pooled across all measured taxa showed different patterns in macromolecular composition compared to those for individual taxon. This study demonstrates that taxon-specific responses to Fe enrichment may not always be accurately reflected by bulk community measurements, highlighting the need for further ... Article in Journal/Newspaper Southern Ocean Directory of Open Access Journals: DOAJ Articles Kerguelen Kerguelen Island ENVELOPE(69.500,69.500,-49.250,-49.250) Southern Ocean Biogeosciences 11 20 5795 5808 |
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Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
spellingShingle |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 O. Sackett L. Armand J. Beardall R. Hill M. Doblin C. Connelly J. Howes B. Stuart P. Ralph P. Heraud Taxon-specific responses of Southern Ocean diatoms to Fe enrichment revealed by synchrotron radiation FTIR microspectroscopy |
topic_facet |
Ecology QH540-549.5 Life QH501-531 Geology QE1-996.5 |
description |
Photosynthesis by marine diatoms contributes substantially to global biogeochemical cycling and ecosystem productivity. It is widely accepted that diatoms are extremely sensitive to changes in Fe availability, with numerous in situ experiments demonstrating rapid growth and increased export of elements (e.g. C, Si and Fe) from surface waters as a result of Fe addition. Less is known about the effects of Fe enrichment on the phenotypes of diatoms, such as associated changes in nutritional value – furthermore, data on taxon-specific responses are almost non-existent. Enhanced supply of nutrient-rich waters along the coast of the subantarctic Kerguelen Island provide a valuable opportunity to examine the responses of phytoplankton to natural Fe enrichment. Here we demonstrate the use of synchrotron radiation Fourier Transform Infrared (SR-FTIR) microspectroscopy to analyse changes in the macromolecular composition of diatoms collected along the coast and plateau of Kerguelen Island, Southern Ocean. SR-FTIR microspectroscopy enabled the analysis of individual diatom cells from mixed communities of field-collected samples, thereby providing insight into in situ taxon-specific responses in relation to changes in Fe availability. Phenotypic responses were taxon-specific in terms of intraspecific variability and changes in proteins, amino acids, phosphorylated molecules, silicate/silicic acid and carbohydrates. In contrast to some previous studies, silicate/silicic acid levels increased under Fe enrichment, in conjunction with increases in carbohydrate stores. The highly abundant taxon Fragilariopsis kerguelensis displayed a higher level of phenotypic plasticity than Pseudo-nitzschia spp., while analysis of the data pooled across all measured taxa showed different patterns in macromolecular composition compared to those for individual taxon. This study demonstrates that taxon-specific responses to Fe enrichment may not always be accurately reflected by bulk community measurements, highlighting the need for further ... |
format |
Article in Journal/Newspaper |
author |
O. Sackett L. Armand J. Beardall R. Hill M. Doblin C. Connelly J. Howes B. Stuart P. Ralph P. Heraud |
author_facet |
O. Sackett L. Armand J. Beardall R. Hill M. Doblin C. Connelly J. Howes B. Stuart P. Ralph P. Heraud |
author_sort |
O. Sackett |
title |
Taxon-specific responses of Southern Ocean diatoms to Fe enrichment revealed by synchrotron radiation FTIR microspectroscopy |
title_short |
Taxon-specific responses of Southern Ocean diatoms to Fe enrichment revealed by synchrotron radiation FTIR microspectroscopy |
title_full |
Taxon-specific responses of Southern Ocean diatoms to Fe enrichment revealed by synchrotron radiation FTIR microspectroscopy |
title_fullStr |
Taxon-specific responses of Southern Ocean diatoms to Fe enrichment revealed by synchrotron radiation FTIR microspectroscopy |
title_full_unstemmed |
Taxon-specific responses of Southern Ocean diatoms to Fe enrichment revealed by synchrotron radiation FTIR microspectroscopy |
title_sort |
taxon-specific responses of southern ocean diatoms to fe enrichment revealed by synchrotron radiation ftir microspectroscopy |
publisher |
Copernicus Publications |
publishDate |
2014 |
url |
https://doi.org/10.5194/bg-11-5795-2014 https://doaj.org/article/a5d62fdfa60d4cd4b7fe4cf162a963a8 |
long_lat |
ENVELOPE(69.500,69.500,-49.250,-49.250) |
geographic |
Kerguelen Kerguelen Island Southern Ocean |
geographic_facet |
Kerguelen Kerguelen Island Southern Ocean |
genre |
Southern Ocean |
genre_facet |
Southern Ocean |
op_source |
Biogeosciences, Vol 11, Iss 20, Pp 5795-5808 (2014) |
op_relation |
http://www.biogeosciences.net/11/5795/2014/bg-11-5795-2014.pdf https://doaj.org/toc/1726-4170 https://doaj.org/toc/1726-4189 1726-4170 1726-4189 doi:10.5194/bg-11-5795-2014 https://doaj.org/article/a5d62fdfa60d4cd4b7fe4cf162a963a8 |
op_doi |
https://doi.org/10.5194/bg-11-5795-2014 |
container_title |
Biogeosciences |
container_volume |
11 |
container_issue |
20 |
container_start_page |
5795 |
op_container_end_page |
5808 |
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1766206594066939904 |