Identification of Algerian Field-Caught Phlebotomine Sand Fly Vectors by MALDI-TOF MS.
BACKGROUND:Phlebotomine sand flies are known to transmit Leishmania parasites, bacteria and viruses that affect humans and animals in many countries worldwide. Precise sand fly identification is essential to prevent phlebotomine-borne diseases. Over the past two decades, progress in matrix-assisted...
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ftdoajarticles:oai:doaj.org/article:a5d5dca2192542de9f06e4fb326f35c6 2023-05-15T15:14:40+02:00 Identification of Algerian Field-Caught Phlebotomine Sand Fly Vectors by MALDI-TOF MS. Ismail Lafri Lionel Almeras Idir Bitam Aurelia Caputo Amina Yssouf Claire-Lise Forestier Arezki Izri Didier Raoult Philippe Parola 2016-01-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0004351 https://doaj.org/article/a5d5dca2192542de9f06e4fb326f35c6 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC4714931?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004351 https://doaj.org/article/a5d5dca2192542de9f06e4fb326f35c6 PLoS Neglected Tropical Diseases, Vol 10, Iss 1, p e0004351 (2016) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2016 ftdoajarticles https://doi.org/10.1371/journal.pntd.0004351 2022-12-31T16:28:11Z BACKGROUND:Phlebotomine sand flies are known to transmit Leishmania parasites, bacteria and viruses that affect humans and animals in many countries worldwide. Precise sand fly identification is essential to prevent phlebotomine-borne diseases. Over the past two decades, progress in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has emerged as an accurate tool for arthropod identification. The objective of the present study was to investigate the usefulness of MALDI-TOF MS as a tool for identifying field-caught phlebotomine. METHODOLOGY/PRINCIPAL FINDINGS:Sand flies were captured in four sites in north Algeria. A subset was morphologically and genetically identified. Six species were found in these areas and a total of 28 stored frozen specimens were used for the creation of the reference spectrum database. The relevance of this original method for sand fly identification was validated by two successive blind tests including the morphological identification of 80 new specimens which were stored at -80°C, and 292 unknown specimens, including engorged specimens, which were preserved under different conditions. Intra-species reproducibility and inter-species specificity of the protein profiles were obtained, allowing us to distinguish specimens at the gender level. Querying of the sand fly database using the MS spectra from the blind test groups revealed concordant results between morphological and MALDI-TOF MS identification. However, MS identification results were less efficient for specimens which were engorged or stored in alcohol. Identification of 362 phlebotomine sand flies, captured at four Algerian sites, by MALDI-TOF MS, revealed that the subgenus Larroussius was predominant at all the study sites, except for in M'sila where P. (Phlebotomus) papatasi was the only sand fly species detected. CONCLUSION:The present study highlights the application of MALDI-TOF MS for monitoring sand fly fauna captured in the field. The low cost, reliability and rapidity of ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLOS Neglected Tropical Diseases 10 1 e0004351 |
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Open Polar |
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ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Ismail Lafri Lionel Almeras Idir Bitam Aurelia Caputo Amina Yssouf Claire-Lise Forestier Arezki Izri Didier Raoult Philippe Parola Identification of Algerian Field-Caught Phlebotomine Sand Fly Vectors by MALDI-TOF MS. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
BACKGROUND:Phlebotomine sand flies are known to transmit Leishmania parasites, bacteria and viruses that affect humans and animals in many countries worldwide. Precise sand fly identification is essential to prevent phlebotomine-borne diseases. Over the past two decades, progress in matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has emerged as an accurate tool for arthropod identification. The objective of the present study was to investigate the usefulness of MALDI-TOF MS as a tool for identifying field-caught phlebotomine. METHODOLOGY/PRINCIPAL FINDINGS:Sand flies were captured in four sites in north Algeria. A subset was morphologically and genetically identified. Six species were found in these areas and a total of 28 stored frozen specimens were used for the creation of the reference spectrum database. The relevance of this original method for sand fly identification was validated by two successive blind tests including the morphological identification of 80 new specimens which were stored at -80°C, and 292 unknown specimens, including engorged specimens, which were preserved under different conditions. Intra-species reproducibility and inter-species specificity of the protein profiles were obtained, allowing us to distinguish specimens at the gender level. Querying of the sand fly database using the MS spectra from the blind test groups revealed concordant results between morphological and MALDI-TOF MS identification. However, MS identification results were less efficient for specimens which were engorged or stored in alcohol. Identification of 362 phlebotomine sand flies, captured at four Algerian sites, by MALDI-TOF MS, revealed that the subgenus Larroussius was predominant at all the study sites, except for in M'sila where P. (Phlebotomus) papatasi was the only sand fly species detected. CONCLUSION:The present study highlights the application of MALDI-TOF MS for monitoring sand fly fauna captured in the field. The low cost, reliability and rapidity of ... |
format |
Article in Journal/Newspaper |
author |
Ismail Lafri Lionel Almeras Idir Bitam Aurelia Caputo Amina Yssouf Claire-Lise Forestier Arezki Izri Didier Raoult Philippe Parola |
author_facet |
Ismail Lafri Lionel Almeras Idir Bitam Aurelia Caputo Amina Yssouf Claire-Lise Forestier Arezki Izri Didier Raoult Philippe Parola |
author_sort |
Ismail Lafri |
title |
Identification of Algerian Field-Caught Phlebotomine Sand Fly Vectors by MALDI-TOF MS. |
title_short |
Identification of Algerian Field-Caught Phlebotomine Sand Fly Vectors by MALDI-TOF MS. |
title_full |
Identification of Algerian Field-Caught Phlebotomine Sand Fly Vectors by MALDI-TOF MS. |
title_fullStr |
Identification of Algerian Field-Caught Phlebotomine Sand Fly Vectors by MALDI-TOF MS. |
title_full_unstemmed |
Identification of Algerian Field-Caught Phlebotomine Sand Fly Vectors by MALDI-TOF MS. |
title_sort |
identification of algerian field-caught phlebotomine sand fly vectors by maldi-tof ms. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2016 |
url |
https://doi.org/10.1371/journal.pntd.0004351 https://doaj.org/article/a5d5dca2192542de9f06e4fb326f35c6 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 10, Iss 1, p e0004351 (2016) |
op_relation |
http://europepmc.org/articles/PMC4714931?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0004351 https://doaj.org/article/a5d5dca2192542de9f06e4fb326f35c6 |
op_doi |
https://doi.org/10.1371/journal.pntd.0004351 |
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PLOS Neglected Tropical Diseases |
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10 |
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1 |
container_start_page |
e0004351 |
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