Investigation into Cryptosporidium and Giardia in bivalve mollusks farmed in Sardinia region and destined for human consumption
Cryptosporidium and Giardia are protozoan parasites transmitted by fecal-oral ingestion of (oo)cysts, and are responsible for enteritis in several animal species and humans worldwide. These (oo)cysts can survive for over a year in aquatic environments and can accumulate in bivalve mollusks, which fi...
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ftdoajarticles:oai:doaj.org/article:a5cd4575ee574375b361df59eeb6ed08 2023-05-15T15:58:01+02:00 Investigation into Cryptosporidium and Giardia in bivalve mollusks farmed in Sardinia region and destined for human consumption Tiziana Tedde Gabriella Piras Sara Salza Rosa Maria Nives Giovanna Sanna Sebastiana Tola Jacopo Culurgioni Cristina Piras Paolo Merella Giovanni Garippa Sebastiano Virgilio 2013-06-01T00:00:00Z https://doi.org/10.4081/ijfs.2013.e26 https://doaj.org/article/a5cd4575ee574375b361df59eeb6ed08 EN IT eng ita PAGEPress Publications http://www.pagepressjournals.org/index.php/ijfs/article/view/998 https://doaj.org/toc/2239-7132 2239-7132 doi:10.4081/ijfs.2013.e26 https://doaj.org/article/a5cd4575ee574375b361df59eeb6ed08 Italian Journal of Food Safety, Vol 2, Iss 2, Pp e26-e26 (2013) Cryptosporidium sp. Giardia sp. Bivalve Staining PCR Food processing and manufacture TP368-456 article 2013 ftdoajarticles https://doi.org/10.4081/ijfs.2013.e26 2022-12-31T03:31:03Z Cryptosporidium and Giardia are protozoan parasites transmitted by fecal-oral ingestion of (oo)cysts, and are responsible for enteritis in several animal species and humans worldwide. These (oo)cysts can survive for over a year in aquatic environments and can accumulate in bivalve mollusks, which filter large volumes of water. The aim of this study is to evaluate the natural occurrence of Cryptosporidium and Giardia contamination in different specimens of edible bivalves mollusks from farming sites of the western and north-eastern coasts of Sardinia. From April 2011 to February 2012, 1095 specimens of Mytilus galloprovincialis and 240 of Crassostrea gigas were sampled from Olbia and Oristano gulf and San Teodoro pond. Hepatopancreas and gills, including the labial palp, were examined for oocysts and cysts after pooling and homogenisation using different techniques: i) staining for light and fluorescence microscopy; ii) direct immunofluorescence (IF) Merifluor® test Cryptosporidium/ Giardia (Meridian Bioscience Inc., Cincinnati, OH, USA); and iii) molecular procedures. However, in the context under study, all mollusks examined with the three main diagnostic techniques were negative for both parasites pointing out the hypothetically low zoonotic risk related to Cryptosporidium and Giardia in bivalves, especially Mytilus galloprovincialis and Crassostrea gigas. Article in Journal/Newspaper Crassostrea gigas Directory of Open Access Journals: DOAJ Articles Italian Journal of Food Safety 2 2 26 |
institution |
Open Polar |
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Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English Italian |
topic |
Cryptosporidium sp. Giardia sp. Bivalve Staining PCR Food processing and manufacture TP368-456 |
spellingShingle |
Cryptosporidium sp. Giardia sp. Bivalve Staining PCR Food processing and manufacture TP368-456 Tiziana Tedde Gabriella Piras Sara Salza Rosa Maria Nives Giovanna Sanna Sebastiana Tola Jacopo Culurgioni Cristina Piras Paolo Merella Giovanni Garippa Sebastiano Virgilio Investigation into Cryptosporidium and Giardia in bivalve mollusks farmed in Sardinia region and destined for human consumption |
topic_facet |
Cryptosporidium sp. Giardia sp. Bivalve Staining PCR Food processing and manufacture TP368-456 |
description |
Cryptosporidium and Giardia are protozoan parasites transmitted by fecal-oral ingestion of (oo)cysts, and are responsible for enteritis in several animal species and humans worldwide. These (oo)cysts can survive for over a year in aquatic environments and can accumulate in bivalve mollusks, which filter large volumes of water. The aim of this study is to evaluate the natural occurrence of Cryptosporidium and Giardia contamination in different specimens of edible bivalves mollusks from farming sites of the western and north-eastern coasts of Sardinia. From April 2011 to February 2012, 1095 specimens of Mytilus galloprovincialis and 240 of Crassostrea gigas were sampled from Olbia and Oristano gulf and San Teodoro pond. Hepatopancreas and gills, including the labial palp, were examined for oocysts and cysts after pooling and homogenisation using different techniques: i) staining for light and fluorescence microscopy; ii) direct immunofluorescence (IF) Merifluor® test Cryptosporidium/ Giardia (Meridian Bioscience Inc., Cincinnati, OH, USA); and iii) molecular procedures. However, in the context under study, all mollusks examined with the three main diagnostic techniques were negative for both parasites pointing out the hypothetically low zoonotic risk related to Cryptosporidium and Giardia in bivalves, especially Mytilus galloprovincialis and Crassostrea gigas. |
format |
Article in Journal/Newspaper |
author |
Tiziana Tedde Gabriella Piras Sara Salza Rosa Maria Nives Giovanna Sanna Sebastiana Tola Jacopo Culurgioni Cristina Piras Paolo Merella Giovanni Garippa Sebastiano Virgilio |
author_facet |
Tiziana Tedde Gabriella Piras Sara Salza Rosa Maria Nives Giovanna Sanna Sebastiana Tola Jacopo Culurgioni Cristina Piras Paolo Merella Giovanni Garippa Sebastiano Virgilio |
author_sort |
Tiziana Tedde |
title |
Investigation into Cryptosporidium and Giardia in bivalve mollusks farmed in Sardinia region and destined for human consumption |
title_short |
Investigation into Cryptosporidium and Giardia in bivalve mollusks farmed in Sardinia region and destined for human consumption |
title_full |
Investigation into Cryptosporidium and Giardia in bivalve mollusks farmed in Sardinia region and destined for human consumption |
title_fullStr |
Investigation into Cryptosporidium and Giardia in bivalve mollusks farmed in Sardinia region and destined for human consumption |
title_full_unstemmed |
Investigation into Cryptosporidium and Giardia in bivalve mollusks farmed in Sardinia region and destined for human consumption |
title_sort |
investigation into cryptosporidium and giardia in bivalve mollusks farmed in sardinia region and destined for human consumption |
publisher |
PAGEPress Publications |
publishDate |
2013 |
url |
https://doi.org/10.4081/ijfs.2013.e26 https://doaj.org/article/a5cd4575ee574375b361df59eeb6ed08 |
genre |
Crassostrea gigas |
genre_facet |
Crassostrea gigas |
op_source |
Italian Journal of Food Safety, Vol 2, Iss 2, Pp e26-e26 (2013) |
op_relation |
http://www.pagepressjournals.org/index.php/ijfs/article/view/998 https://doaj.org/toc/2239-7132 2239-7132 doi:10.4081/ijfs.2013.e26 https://doaj.org/article/a5cd4575ee574375b361df59eeb6ed08 |
op_doi |
https://doi.org/10.4081/ijfs.2013.e26 |
container_title |
Italian Journal of Food Safety |
container_volume |
2 |
container_issue |
2 |
container_start_page |
26 |
_version_ |
1766393733600772096 |