Anti-schistosomal intervention targets identified by lifecycle transcriptomic analyses.
Novel methods to identify anthelmintic drug and vaccine targets are urgently needed, especially for those parasite species currently being controlled by singular, often limited strategies. A clearer understanding of the transcriptional components underpinning helminth development will enable identif...
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ftdoajarticles:oai:doaj.org/article:a5ab8a53c17540caaf325d8da17762f9 2023-05-15T15:10:37+02:00 Anti-schistosomal intervention targets identified by lifecycle transcriptomic analyses. Jennifer M Fitzpatrick Emily Peak Samirah Perally Iain W Chalmers John Barrett Timothy P Yoshino Alasdair C Ivens Karl F Hoffmann 2009-11-01T00:00:00Z https://doi.org/10.1371/journal.pntd.0000543 https://doaj.org/article/a5ab8a53c17540caaf325d8da17762f9 EN eng Public Library of Science (PLoS) http://europepmc.org/articles/PMC2764848?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000543 https://doaj.org/article/a5ab8a53c17540caaf325d8da17762f9 PLoS Neglected Tropical Diseases, Vol 3, Iss 11, p e543 (2009) Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 article 2009 ftdoajarticles https://doi.org/10.1371/journal.pntd.0000543 2022-12-31T00:46:27Z Novel methods to identify anthelmintic drug and vaccine targets are urgently needed, especially for those parasite species currently being controlled by singular, often limited strategies. A clearer understanding of the transcriptional components underpinning helminth development will enable identification of exploitable molecules essential for successful parasite/host interactions. Towards this end, we present a combinatorial, bioinformatics-led approach, employing both statistical and network analyses of transcriptomic data, for identifying new immunoprophylactic and therapeutic lead targets to combat schistosomiasis.Utilisation of a Schistosoma mansoni oligonucleotide DNA microarray consisting of 37,632 elements enabled gene expression profiling from 15 distinct parasite lifecycle stages, spanning three unique ecological niches. Statistical approaches of data analysis revealed differential expression of 973 gene products that minimally describe the three major characteristics of schistosome development: asexual processes within intermediate snail hosts, sexual maturation within definitive vertebrate hosts and sexual dimorphism amongst adult male and female worms. Furthermore, we identified a group of 338 constitutively expressed schistosome gene products (including 41 transcripts sharing no sequence similarity outside the Platyhelminthes), which are likely to be essential for schistosome lifecycle progression. While highly informative, statistics-led bioinformatics mining of the transcriptional dataset has limitations, including the inability to identify higher order relationships between differentially expressed transcripts and lifecycle stages. Network analysis, coupled to Gene Ontology enrichment investigations, facilitated a re-examination of the dataset and identified 387 clusters (containing 12,132 gene products) displaying novel examples of developmentally regulated classes (including 294 schistosomula and/or adult transcripts with no known sequence similarity outside the Platyhelminthes), which were ... Article in Journal/Newspaper Arctic Directory of Open Access Journals: DOAJ Articles Arctic PLoS Neglected Tropical Diseases 3 11 e543 |
institution |
Open Polar |
collection |
Directory of Open Access Journals: DOAJ Articles |
op_collection_id |
ftdoajarticles |
language |
English |
topic |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
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Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 Jennifer M Fitzpatrick Emily Peak Samirah Perally Iain W Chalmers John Barrett Timothy P Yoshino Alasdair C Ivens Karl F Hoffmann Anti-schistosomal intervention targets identified by lifecycle transcriptomic analyses. |
topic_facet |
Arctic medicine. Tropical medicine RC955-962 Public aspects of medicine RA1-1270 |
description |
Novel methods to identify anthelmintic drug and vaccine targets are urgently needed, especially for those parasite species currently being controlled by singular, often limited strategies. A clearer understanding of the transcriptional components underpinning helminth development will enable identification of exploitable molecules essential for successful parasite/host interactions. Towards this end, we present a combinatorial, bioinformatics-led approach, employing both statistical and network analyses of transcriptomic data, for identifying new immunoprophylactic and therapeutic lead targets to combat schistosomiasis.Utilisation of a Schistosoma mansoni oligonucleotide DNA microarray consisting of 37,632 elements enabled gene expression profiling from 15 distinct parasite lifecycle stages, spanning three unique ecological niches. Statistical approaches of data analysis revealed differential expression of 973 gene products that minimally describe the three major characteristics of schistosome development: asexual processes within intermediate snail hosts, sexual maturation within definitive vertebrate hosts and sexual dimorphism amongst adult male and female worms. Furthermore, we identified a group of 338 constitutively expressed schistosome gene products (including 41 transcripts sharing no sequence similarity outside the Platyhelminthes), which are likely to be essential for schistosome lifecycle progression. While highly informative, statistics-led bioinformatics mining of the transcriptional dataset has limitations, including the inability to identify higher order relationships between differentially expressed transcripts and lifecycle stages. Network analysis, coupled to Gene Ontology enrichment investigations, facilitated a re-examination of the dataset and identified 387 clusters (containing 12,132 gene products) displaying novel examples of developmentally regulated classes (including 294 schistosomula and/or adult transcripts with no known sequence similarity outside the Platyhelminthes), which were ... |
format |
Article in Journal/Newspaper |
author |
Jennifer M Fitzpatrick Emily Peak Samirah Perally Iain W Chalmers John Barrett Timothy P Yoshino Alasdair C Ivens Karl F Hoffmann |
author_facet |
Jennifer M Fitzpatrick Emily Peak Samirah Perally Iain W Chalmers John Barrett Timothy P Yoshino Alasdair C Ivens Karl F Hoffmann |
author_sort |
Jennifer M Fitzpatrick |
title |
Anti-schistosomal intervention targets identified by lifecycle transcriptomic analyses. |
title_short |
Anti-schistosomal intervention targets identified by lifecycle transcriptomic analyses. |
title_full |
Anti-schistosomal intervention targets identified by lifecycle transcriptomic analyses. |
title_fullStr |
Anti-schistosomal intervention targets identified by lifecycle transcriptomic analyses. |
title_full_unstemmed |
Anti-schistosomal intervention targets identified by lifecycle transcriptomic analyses. |
title_sort |
anti-schistosomal intervention targets identified by lifecycle transcriptomic analyses. |
publisher |
Public Library of Science (PLoS) |
publishDate |
2009 |
url |
https://doi.org/10.1371/journal.pntd.0000543 https://doaj.org/article/a5ab8a53c17540caaf325d8da17762f9 |
geographic |
Arctic |
geographic_facet |
Arctic |
genre |
Arctic |
genre_facet |
Arctic |
op_source |
PLoS Neglected Tropical Diseases, Vol 3, Iss 11, p e543 (2009) |
op_relation |
http://europepmc.org/articles/PMC2764848?pdf=render https://doaj.org/toc/1935-2727 https://doaj.org/toc/1935-2735 1935-2727 1935-2735 doi:10.1371/journal.pntd.0000543 https://doaj.org/article/a5ab8a53c17540caaf325d8da17762f9 |
op_doi |
https://doi.org/10.1371/journal.pntd.0000543 |
container_title |
PLoS Neglected Tropical Diseases |
container_volume |
3 |
container_issue |
11 |
container_start_page |
e543 |
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1766341605207310336 |